scholarly journals Purification and Characterization of Δ1-Pyrroline-5-Carboxylate Reductase Isoenzymes, Indicating Differential Distribution in Spinach (Spinacia oleracea L.) Leaves

2001 ◽  
Vol 42 (7) ◽  
pp. 742-750 ◽  
Author(s):  
Minoru Murahama ◽  
Takayuki Yoshida ◽  
Fumio Hayashi ◽  
Takuya Ichino ◽  
Yukika Sanada ◽  
...  
Keyword(s):  
Spinacia Oleracea ◽  
Purification And Characterization ◽  
Differential Distribution ◽  
Spinacia Oleracea L ◽  
Carboxylate Reductase

Purification and characterization of a functionally active Mycobacterium tuberculosis pyrroline-5-carboxylate reductase

2006 ◽  
Vol 45 (1) ◽  
pp. 241-248 ◽  
Author(s):  
Yanping Yang ◽  
Shengfeng Xu ◽  
Min Zhang ◽  
Ruiliang Jin ◽  
Lu Zhang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Purification And Characterization ◽  
Carboxylate Reductase

Characterization of Multiple Acid Phosphatases in Salt-Stressed Spinach Leaves

1987 ◽  
Vol 14 (2) ◽  
pp. 117 ◽  
Author(s):  
SM Pan
Keyword(s):  
Molecular Weight ◽  
Salt Stress ◽  
Gel Electrophoresis ◽  
Spinacia Oleracea ◽  
Acid Phosphatases ◽  
Multiple Forms ◽  
Spinacia Oleracea L ◽  
Hydroponically Grown ◽  
Spinach Leaves

Incremental salt stress brought about a clear enhancement of the activity of acid phosphatases in hydroponically grown spinach (Spinacia oleracea L.) leaves. Sephacryl S-200 chromatography of the enzyme fraction revealed multiple forms of acid phosphatases of high (300 000), intermediate (100 000), and low (35 000) molecular weight in control and salt-stressed spinach leaves. A similar zymogram of acid phosphatases, showing at least six bands in polyacrylamide disc gel electrophoresis, was observed for control and stressed leaves. However, promotive effects of incremental salt stress on the activity of acid phosphatases were more pronounced in high molecular weight acid phosphatases. Substrate specificity and differential effects of some ions on the multiple acid phosphatases were also examined for control and salt-stressed leaves.

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