scholarly journals Characterization of an Isoform of Rice Starch Branching Enzyme, RBE4, in Developing Seeds

2001 ◽  
Vol 42 (4) ◽  
pp. 349-357 ◽  
Author(s):  
K. Mizuno
Keyword(s):  
Rice Starch ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Developing Seeds

Production of High Amylose and Resistant Starch Rice Through Targeted Mutagenesis of Starch Branching Enzyme Iib by Crispr/cas9

2021 ◽  
Author(s):  
Hsi-Chao Wang ◽  
Yu-Chia Hsu ◽  
Yong-Pei Wu ◽  
Su-Ying Yeh ◽  
Maurice S. B. Ku
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Resistant Starch ◽  
Targeted Mutagenesis ◽  
Rice Starch ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Homozygous Mutant ◽  
Mutant Lines ◽  
High Amylose

Abstract Rice is the staple food for half of the world’s population. Starch accounts for 80-90% of the total mass of rice seeds, and rice starch is low in resistant starch (RS) with a high glycemic index (GI). RS has gained important since it is beneficial in preventing various diseases. Starch branching enzyme IIb (SBEIIb) plays a key role in the amylopectin synthesis pathway in the endosperm of cereals. Down-regulation of SBEIIb in several important crops has led to high amylose, high RS and low GI starch. In this study, we mutated OsSBEIIb in the japonica rice cultivar TNG82 through CRISPR/Cas9 and investigated the molecular and physicochemical modifications in OsSBEIIb mutant lines, e.g., gene expression, enzyme activity, apparent amylose content (AAC), RS and GI. As expected, the levels of modification in these starch related traits in heterozygous mutant lines were about half as those of homozygous mutant lines. Gene expression and enzyme activity of OsSBEIIb were down-regulated significantly while AAC and RS contents increased progressively from 17.4% and 0.5% in WT, respectively, to as high as 25.0% and 7.5% in heterozygous mutant lines and 36.0% and 12.0% in homozygous mutant lines. Consequently, with increased RS and decreased rate of reducing sugar production, GI progressively decreased in heterozygous and homozygous mutant rice endosperms by 11% and 28%, respectively. Our results demonstrate that it has huge potential for precise and efficient generation of high RS and low GI rice through CRISPR/Cas9 to provide a more suitable source of starch for type II diabetes.

scholarly journals Development and characterization of a new rice cultivar, ‘Chikushi-kona 85’, derived from a starch-branching enzyme IIb-deficient mutant line

2018 ◽  
Vol 68 (2) ◽  
pp. 278-283 ◽  
Author(s):  
Takuya Wada ◽  
Osamu Yamaguchi ◽  
Masayuki Miyazaki ◽  
Katsunori Miyahara ◽  
Masafumi Ishibashi ◽  
...  
Keyword(s):  
Rice Cultivar ◽  
Mutant Line ◽  
Deficient Mutant ◽  
Branching Enzyme ◽  
Starch Branching Enzyme

Molecular Characterization of Mungbean (Vigna radiataL.) Starch Branching Enzyme I

2010 ◽  
Vol 58 (19) ◽  
pp. 10437-10444 ◽  
Author(s):  
Jia-Wei Chang ◽  
Sing-Chung Li ◽  
Yun-Chi Shih ◽  
Reuben Wang ◽  
Pei-Shan Chung ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Enzyme I

scholarly journals Transcriptome wide identification and characterization of starch branching enzyme in finger millet

2017 ◽  
Vol 13 (06) ◽  
pp. 179-184 ◽  
Author(s):  
Rajhans Tyagi ◽  
◽  
Apoorv Tiwari ◽  
Vijay Kumar Garg ◽  
Sanjay Gupta ◽  
...  
Keyword(s):  
Finger Millet ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Identification And Characterization

scholarly journals Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri

2017 ◽  
Vol 618 ◽  
pp. 52-61 ◽  
Author(s):  
Nicolas Hedin ◽  
Julieta Barchiesi ◽  
Diego F. Gomez-Casati ◽  
Alberto A. Iglesias ◽  
Miguel A. Ballicora ◽  
...  
Keyword(s):  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Ostreococcus Tauri ◽  
Identification And Characterization ◽  
Novel Starch

Genomic isolation of genes encoding starch branching enzyme II (SBEII) in apple: toward characterization of evolutionary disparity in SbeII genes between monocots and eudicots

Planta ◽  
2007 ◽  
Vol 226 (5) ◽  
pp. 1265-1276 ◽  
Author(s):  
Yuepeng Han ◽  
Elise Bendik ◽  
Feng-Jie Sun ◽  
Ksenija Gasic ◽  
Schuyler S. Korban
Keyword(s):  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Genes Encoding
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