scholarly journals Isolation and Characterization of cDNAs for Differentially Accumulated Transcripts between Mesophyll Cells and Bundle Sheath Strands of Maize Leaves

2000 ◽  
Vol 41 (11) ◽  
pp. 1200-1209 ◽  
Author(s):  
Tsuyoshi Furumoto ◽  
Shingo Hata ◽  
Katsura Izui
Keyword(s):  
Bundle Sheath ◽  
Mesophyll Cells ◽  
Isolation And Characterization ◽  
Maize Leaves

Control of cellular differentiation in maize leaves

1995 ◽  
Vol 350 (1331) ◽  
pp. 53-57 ◽  
Keyword(s):  
Cellular Differentiation ◽  
Functional Characterization ◽  
Cell Types ◽  
Bundle Sheath ◽  
Gene Products ◽  
Mesophyll Cells ◽  
Bundle Sheath Cells ◽  
Maize Leaves ◽  
Single Cell Type

Mature maize leaves exhibit a series of parallel veins that are surrounded by concentric rings of bundle sheath and mesophyll cells. To identify genes that control cellular differentiation patterns in the leaf, we have isolated a group of mutations that specifically disrupt the differentiation of a single cell-type. In bundle sheath defective ( bsd ) mutant plants, bundle sheath cells fail to differentiate yet mesophyll and all other leaf cell-types develop normally. Morphological and functional characterization of specific bsd mutants ( bsd1, bsd2, bsd3, pg14 and g2 ) reveals that they differ in the degree to which bundle sheath cell differentiation is perturbed. Mutant analysis predicts roles for BSD gene products in normal development.

scholarly journals Cyst(e)ine Is the Transport Metabolite of Assimilated Sulfur from Bundle-Sheath to Mesophyll Cells in Maize Leaves

1998 ◽  
Vol 116 (4) ◽  
pp. 1315-1322 ◽  
Author(s):  
Marta Burgener ◽  
Marianne Suter ◽  
Stephanie Jones ◽  
Christian Brunold
Keyword(s):  
Bundle Sheath ◽  
Mesophyll Cells ◽  
Maize Leaves

scholarly journals Complementary DNA Cloning and Characterization of Ferredoxin Localized in Bundle-Sheath Cells of Maize Leaves

1999 ◽  
Vol 119 (2) ◽  
pp. 481-488 ◽  
Author(s):  
Tomohiro Matsumura ◽  
Yoko Kimata-Ariga ◽  
Hitoshi Sakakibara ◽  
Tatsuo Sugiyama ◽  
Hiroshi Murata ◽  
...  
Keyword(s):  
Bundle Sheath ◽  
Dna Cloning ◽  
Complementary Dna ◽  
Bundle Sheath Cells ◽  
Maize Leaves ◽  
Complementary Dna Cloning ◽  
Sheath Cells

scholarly journals Implication of the glutamine synthetase/glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves

FEBS Journal ◽  
2008 ◽  
Vol 275 (12) ◽  
pp. 3193-3206 ◽  
Author(s):  
Marie-Hélène Valadier ◽  
Ayako Yoshida ◽  
Olivier Grandjean ◽  
Halima Morin ◽  
Jocelyne Kronenberger ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glutamine Synthetase ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Glutamate Synthase ◽  
Bundle Sheath ◽  
Mesophyll Cells ◽  
Maize Leaves

scholarly journals Detection of phosphoenolpyruvate and ribulose 1,5-bisphosphate carboxylase transcripts in maize leaves by in situ hybridization with sulfonated cDNA probes.

1989 ◽  
Vol 37 (4) ◽  
pp. 423-428 ◽  
Author(s):  
C Perrot-Rechenmann ◽  
M Joannes ◽  
D Squalli ◽  
P Lebacq
Keyword(s):  
In Situ Hybridization ◽  
Cell Types ◽  
Small Subunit ◽  
Bundle Sheath ◽  
Differential Distribution ◽  
Mesophyll Cells ◽  
Bisphosphate Carboxylase ◽  
Maize Leaves ◽  
Specific Mrnas

This report outlines an efficient in situ hybridization method for locating specific mRNAs in tissue cryosections using sulfonated cDNA probes. The method involves chemical modification of DNA probes by insertion of a sulfone radical on cytosine residues, which generates a specific epitope. Sulfonated DNA is then detected by using indirect immunochemical procedure. Alternatively, antibodies conjugated to fluorescein or to alkaline phosphatase were used for mRNA detection. In situ hybridization was developed to study aspects of mesophyll and bundle sheath cell differentiation in maize leaves. Our results indicate that phosphoenolpyruvate carboxylase (PEP C) mRNA is restricted to mesophyll cells, and the nucleus-encoded mRNA of the small subunit (SSU) ribulose 1,5-bisphosphate carboxylase (RuBP C) is limited to the cytosol of bundle sheath cells. Thus, using in situ hybridization, we have demonstrated that the differential distribution of PEP C and RuBP C proteins in the two cell types also reflects the location of their mRNAs. These data imply either a tissue-specific transcriptional regulation or a selective mRNA degradation.

scholarly journals Ultrastructural Characterization of Infection and Colonization of Maize Leaves by Colletotrichum graminicola, and by a C. graminicola Pathogenicity Mutant

2002 ◽  
Vol 92 (7) ◽  
pp. 803-812 ◽  
Author(s):  
C. W. Mims ◽  
L. J. Vaillancourt
Keyword(s):  
Host Cells ◽  
Signal Peptidase ◽  
Colletotrichum Graminicola ◽  
Wild Type ◽  
Mesophyll Cells ◽  
Ultrastructural Characterization ◽  
Significant Difference ◽  
Maize Leaves ◽  
Short Time

Observations were made of the ultrastructure of infection and colonization of leaves of a susceptible maize inbred by Colletotrichum graminicola and by a C. graminicola pathogenicity mutant. The mutant causes no symptoms on either maize leaves or stalks. Prior evidence suggested that it is deficient in production of signal peptidase, responsible for cleavage of signal peptides from proteins destined for transport through the endoplasmic reticulum. There was no significant difference in the process of infection or colonization by the mutant and wild-type strains up to 48 h after inoculation. Both the mutant and the wild type produced globose, melanized appressoria within 24 h after inoculation on the host surface. By 36 h, both strains had penetrated the host epidermal cells directly. The host cells frequently formed papillae in response to appressoria, but these were not usually successful in preventing fungal ingress in either case. Penetration was followed by formation of irregularly shaped, swollen infection hyphae. Infection hyphae of both strains grew biotrophically for a relatively short time (less than 12 h). One or more hyphal branches was produced from each infection hypha, and these invaded adjacent mesophyll cells. Both strains of the fungus grew cell-to-cell, setting up new biotrophic interactions in each cell, between 36 and 48 h after inoculation. Papillae were frequently formed by the mesophyll cells, but these were not successful in preventing fungal ingress. The first noticeable difference between the mutant and the wild type was related to their interaction with mesophyll cells. Cells invaded by the wild type died relatively quickly, whereas those infected by the mutant appeared to survive longer. The most dramatic difference between the mutant and wild type occurred when the mutant completely failed to make a transition to necrotrophic growth, while the wild type made that switch at 48 to 72 h after inoculation. The mutant may be unable to secrete sufficient quantities of one or more proteins that are necessary to support the switch between biotrophy and necrotrophy.

bundle sheath defective, a mutation that disrupts cellular differentiation in maize leaves

Development ◽  
1994 ◽  
Vol 120 (3) ◽  
pp. 673-681 ◽  
Author(s):  
J. A. Langdale ◽  
C. A. Kidner
Keyword(s):  
Gene Action ◽  
Morphological Differentiation ◽  
Cell Types ◽  
Bundle Sheath ◽  
Sheath Cell ◽  
Mesophyll Cells ◽  
Bundle Sheath Cell ◽  
Bundle Sheath Cells ◽  
Maize Leaves ◽  
Sheath Cells

Post-primordial differentiation events in developing maize leaves produce two photosynthetic cell types (bundle sheath and mesophyll) that are morphologically and biochemically distinct. We have isolated a mutation that disrupts the differentiation of one of these cell types in light-grown leaves. bundle sheath defective 1-mutable 1 (bsd1-m1) is an unstable allele that was induced by transposon mutagenesis. In the bundle sheath cells of bsd1-m1 leaves, chloroplasts differentiate aberrantly and C4 photosynthetic enzymes are absent. The development of mesophyll cells is unaffected. In dark-grown bsd1-m1 seedlings, morphological differentiation of etioplasts is only disrupted in bundle sheath cells but photosynthetic enzyme accumulation patterns are altered in both cell types. These data suggest that, during normal development, the Bsd1 gene directs the morphological differentiation of chloroplasts in a light-independent and bundle sheath cell-specific fashion. In contrast, Bsd1 gene action on photosynthetic gene expression patterns is cell-type independent in the dark (C3 state) but bundle sheath cell-specific in the light (C4 state). Current models hypothesize that C4 photosynthetic differentiation is achieved through a light-induced interaction between bundle sheath and mesophyll cells (J. A. Langdale and T. Nelson (1991) Trends in Genetics 7, 191–196). Based on the data shown in this paper, we propose that induction of the C4 state restricts Bsd1 gene action to bundle sheath cells.

scholarly journals Pyruvate, Pi Dikinase in Bundle Sheath Strands as Well as in Mesophyll Cells in Maize Leaves

1985 ◽  
Vol 78 (3) ◽  
pp. 661-664 ◽  
Author(s):  
Kazuko Aoyagi ◽  
Hitoshi Nakamoto
Keyword(s):  
Bundle Sheath ◽  
Mesophyll Cells ◽  
Maize Leaves
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