Analysis of the function of the alfalfa MsLEA-D34 gene in abiotic stress responses and flowering time

2020 ◽  
Author(s):  
Aimin Lv ◽  
Liantai Su ◽  
Wuwu Wen ◽  
Nana Fan ◽  
Peng Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Flowering Time ◽  
Hairy Roots ◽  
Stress Responses ◽  
Intrinsically Disordered Protein ◽  
Plant Tolerance ◽  
Abiotic Stress Response ◽  
Regulatory Pathways ◽  
Intrinsically Disordered

Abstract A novel late embryogenesis abundant (LEA) gene, MsLEA-D34, was cloned from alfalfa (Medicago sativa L.). Its function and gene regulatory pathways were studied via overexpression and RNAi of the gene in Arabidopsis and hairy roots of alfalfa, as well as via analyzing key genes related to MsLEA-D34 during developmental phases of alfalfa. The results showed that MsLEA-D34 was a typical intrinsically disordered protein with high ability of protein protection. Overexpression of MsLEA-D34 increased plant tolerance to osmotic and salt stresses, and caused Arabidopsis early flowering under drought and well water conditions. Overexpressing MsLEA-D34 (OE-MsLEA-D34) induced up-regulation of FLOWERING LOCUS T (FT) and GIGANTEA (GI) at flowering phase of Arabidopsis was clearly observed in MsLEA-D34-OE hairy roots of alfalfa, but only FT downregulated in MsLEA-D34-RNAi lines. A positive effect of MsLEA-D34 on FT accumulation was demonstrated in alfalfa hairy roots. Abscisic acid (ABA)-responsive element (ABRE)-binding transcription factor (MsABF2), a novel transcription factor cloned from alfalfa, directly bound to RY element in MsLEA-D34 promoter and activated MsLEA-D34 expression. The above results indicate that MsLEA-D34 can regulate abiotic stress response in plants and influence flowering time of Arabidopsis.

Picea wilsonii transcription factor NAC2 enhanced plant tolerance to abiotic stress and participated in RFCP1-regulated flowering time

2018 ◽  
Vol 98 (6) ◽  
pp. 471-493 ◽  
Author(s):  
Hehua Zhang ◽  
Xiaoyue Cui ◽  
Yuxiao Guo ◽  
Chaobing Luo ◽  
Lingyun Zhang
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Flowering Time ◽  
Plant Tolerance ◽  
Picea Wilsonii

scholarly journals The bZIP Transcription Factor GmbZIP15 Negatively Regulates Salt- and Drought-Stress Responses in Soybean

2020 ◽  
Vol 21 (20) ◽  
pp. 7778
Author(s):  
Man Zhang ◽  
Yanhui Liu ◽  
Hanyang Cai ◽  
Mingliang Guo ◽  
Mengnan Chai ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Responses ◽  
Negative Regulator ◽  
Bzip Transcription Factor ◽  
Antioxidant Enzyme Activities ◽  
Oilseed Crop ◽  
Abiotic Stress Response ◽  
Salt And Drought Stress

Soybean (Glycine max), as an important oilseed crop, is constantly threatened by abiotic stress, including that caused by salinity and drought. bZIP transcription factors (TFs) are one of the largest TF families and have been shown to be associated with various environmental-stress tolerances among species; however, their function in abiotic-stress response in soybean remains poorly understood. Here, we characterized the roles of soybean transcription factor GmbZIP15 in response to abiotic stresses. The transcript level of GmbZIP15 was suppressed under salt- and drought-stress conditions. Overexpression of GmbZIP15 in soybean resulted in hypersensitivity to abiotic stress compared with wild-type (WT) plants, which was associated with lower transcript levels of stress-responsive genes involved in both abscisic acid (ABA)-dependent and ABA-independent pathways, defective stomatal aperture regulation, and reduced antioxidant enzyme activities. Furthermore, plants expressing a functional repressor form of GmbZIP15 exhibited drought-stress resistance similar to WT. RNA-seq and qRT-PCR analyses revealed that GmbZIP15 positively regulates GmSAHH1 expression and negatively regulates GmWRKY12 and GmABF1 expression in response to abiotic stress. Overall, these data indicate that GmbZIP15 functions as a negative regulator in response to salt and drought stresses.

scholarly journals Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance

2020 ◽  
Vol 21 (6) ◽  
pp. 2177 ◽  
Author(s):  
Bo Li ◽  
Jia-Cheng Zheng ◽  
Ting-Ting Wang ◽  
Dong-Hong Min ◽  
Wen-Liang Wei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Hairy Roots ◽  
Yeast Cells ◽  
Salt Stress Tolerance ◽  
Drought And Salt Stress

Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (GmVOZs) have been reported to exist in the soybean (Glycine max) genome. In spite of this, little information is currently available regarding GmVOZs. In this study, GmVOZs were cloned and characterized. GmVOZ genes encode proteins possessing transcriptional activation activity in yeast cells. GmVOZ1E, GmVOZ2B, and GmVOZ2D gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. GmVOZs displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, GmVOZ1G showed a significantly induced expression in response to all stress treatments. Overexpression of GmVOZ1G in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing GmVOZ1G were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, GmVOZ1G positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.

scholarly journals The Construction of Plant Expression Vector harbouring Carica Papaya L. WRKY Gene in Escherichia coli

2020 ◽  
Vol 28 (S2) ◽  
Author(s):  
Fauziah Abu Bakar ◽  
Pavitra Paramalingam ◽  
Kamariah Hasan
Keyword(s):  
Abiotic Stress ◽  
Stress Responses ◽  
Expression Vector ◽  
Carica Papaya ◽  
Sequencing Analysis ◽  
Wrky Gene ◽  
Limited Information ◽  
Abiotic Stress Response ◽  
Plant Expression ◽  
Plant Expression Vector

Carica papaya is a well-liked and economically important fruit with outstanding nutritional and medicinal values. Its susceptibility to abiotic stress which affects the growth and harvest, causes significant yield loss to farmers. In recent years, significant progress has been made to understand the genes that play critical roles in abiotic stress response, especially some transcription factor (TF) encoding genes. Among all TFs, WRKY TF gene family is one of the best-studied TFs involved in various stress responses. To date, only limited information on functionally characterised WRKY TFs is available for C. papaya. The aim of this study was to produce a recombinant construct harbouring WRKY gene in pGEM®-T Easy cloning vector. The presence of a DNA band of the expected size of 465 bp on agarose gel electrophoresis indicated that WRKY gene was successfully amplified from all treated samples. DNA sequencing analysis revealed that the amplified sequence isolated from the treated samples were closely related to Carica papaya species with 97% similarity. Following transformation, 4 out of 5 colonies that were randomly selected showed the WRKY gene had been successfully inserted into pGEM®-T Easy vector and transformed into E. coli. In future, the WRKY gene from pGEMT-WRKY recombinant construct will be cloned into the plant expression vector pCAMBIA 1304 prior to transformation in the plant. The success of demonstrating the WRKY gene towards the response in abiotic stress will enable us to produce stress tolerant transgenic crops under unfavourable conditions via genetic engineering for sustained growth.

An intrinsically disordered protein, CP12: jack of all trades and master of the Calvin cycle

2012 ◽  
Vol 40 (5) ◽  
pp. 995-999 ◽  
Author(s):  
Brigitte Gontero ◽  
Stephen C. Maberly
Keyword(s):  
Amino Acids ◽  
Stress Responses ◽  
Intrinsically Disordered Proteins ◽  
Calvin Cycle ◽  
Intrinsically Disordered Protein ◽  
Disulfide Bridge ◽  
Co2 Assimilation ◽  
Disordered Proteins ◽  
Dimensional Structure ◽  
Intrinsically Disordered

Many proteins contain disordered regions under physiological conditions and lack specific three-dimensional structure. These are referred to as IDPs (intrinsically disordered proteins). CP12 is a chloroplast protein of approximately 80 amino acids and has a molecular mass of approximately 8.2–8.5 kDa. It is enriched in charged amino acids and has a small number of hydrophobic residues. It has a high proportion of disorder-promoting residues, but has at least two (often four) cysteine residues forming one (or two) disulfide bridge(s) under oxidizing conditions that confers some order. However, CP12 behaves like an IDP. It appears to be universally distributed in oxygenic photosynthetic organisms and has recently been detected in a cyanophage. The best studied role of CP12 is its regulation of the Calvin cycle responsible for CO2 assimilation. Oxidized CP12 forms a supramolecular complex with two key Calvin cycle enzymes, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and PRK (phosphoribulokinase), down-regulating their activity. Association–dissociation of this complex, induced by the redox state of CP12, allows the Calvin cycle to be inactive in the dark and active in the light. CP12 is promiscuous and interacts with other enzymes such as aldolase and malate dehydrogenase. It also plays other roles in plant metabolism such as protecting GAPDH from inactivation and scavenging metal ions such as copper and nickel, and it is also linked to stress responses. Thus CP12 seems to be involved in many functions in photosynthetic cells and behaves like a jack of all trades as well as being a master of the Calvin cycle.

scholarly journals Genome-wide study of pineapple (Ananas comosus L.) bHLH transcription factors indicates that cryptochrome-interacting bHLH2 (AcCIB2) participates in flowering time regulation and abiotic stress response

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohammad Aslam ◽  
Bello Hassan Jakada ◽  
Beenish Fakher ◽  
Joseph G. Greaves ◽  
Xiaoping Niu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Stress Response ◽  
Flowering Time ◽  
Current Knowledge ◽  
Ectopic Expression ◽  
Ananas Comosus ◽  
Biological Processes ◽  
Abiotic Stress Response ◽  
Bhlh Genes

Abstract Background Transcription factors (TFs) are essential regulators of growth and development in eukaryotes. Basic-helix-loop-helix (bHLHs) is one of the most significant TFs families involved in several critical regulatory functions. Cryptochrome-interacting bHLH (CIB) and cryptochromes form an extensive regulatory network to mediate a plethora of pathways. Although bHLHs regulate critical biological processes in plants, the information about pineapple bHLHs remains unexplored. Results Here, we identified a total of 121 bHLH proteins in the pineapple genome. The identified genes were renamed based on the ascending order of their gene ID and classified into 18 subgroups by phylogenetic analysis. We found that bHLH genes are expressed in different organs and stages of pineapple development. Furthermore, by the ectopic expression of AcCIB2 in Arabidopsis and complementation of Atcib2 mutant, we verified the involvement of AcCIB2 in photomorphogenesis and abiotic stress response. Conclusions Our findings revealed that AcCIB2 plays an essential role in flowering time regulation and abiotic stress response. The present study provides additional insights into the current knowledge of bHLH genes and suggests their potential role in various biological processes during pineapple development.

scholarly journals The transcription factor YY 2 has less momentous properties of an intrinsically disordered protein than its paralog YY 1

FEBS Letters ◽  
2019 ◽  
Vol 593 (14) ◽  
pp. 1787-1798 ◽  
Author(s):  
Małgorzata Figiel ◽  
Julia Łakomska ◽  
Piotr Miłek ◽  
Marta Dziedzicka‐Wasylewska ◽  
Andrzej Górecki
Keyword(s):  
Transcription Factor ◽  
Intrinsically Disordered Protein ◽  
Intrinsically Disordered ◽  
Disordered Protein

scholarly journals Genome-wide characterization of NtHD-ZIP IV: different roles in abiotic stress response and glandular Trichome induction

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongying Zhang ◽  
Xudong Ma ◽  
Wenjiao Li ◽  
Dexin Niu ◽  
Zhaojun Wang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Stress Responses ◽  
Leucine Zipper ◽  
Regulatory Gene ◽  
Glandular Trichomes ◽  
Glandular Trichome ◽  
Tissue Expression ◽  
Abiotic Stress Response ◽  
Epidermal Development

Abstract Background The plant-specific homeodomain-leucine zipper class IV (HD-ZIP IV) gene family has been involved in the regulation of epidermal development. Results Fifteen genes coding for HD-ZIP IV proteins were identified (NtHD-ZIP-IV-1 to NtHD-ZIP-IV-15) based on the genome of N. tabacum. Four major domains (HD, ZIP, SAD and START) were present in these proteins. Tissue expression pattern analysis indicated that NtHD-ZIP-IV-1, − 2, − 3, − 10, and − 12 may be associated with trichome development; NtHD-ZIP-IV-8 was expressed only in cotyledons; NtHD-ZIP-IV-9 only in the leaf and stem epidermis; NtHD-ZIP-IV-11 only in leaves; and NtHD-ZIP-IV-15 only in the root and stem epidermis. We found that jasmonates may induce the generation of glandular trichomes, and that NtHD-ZIP-IV-1, − 2, − 5, and − 7 were response to MeJA treatment. Dynamic expression under abiotic stress and after application of phytohormones indicated that most NtHD-ZIP IV genes were induced by heat, cold, salt and drought. Furthermore, most of these genes were induced by gibberellic acid, 6-benzylaminopurine, and salicylic acid, but were inhibited by abscisic acid. NtHD-ZIP IV genes were sensitive to heat, but insensitive to osmotic stress. Conclusion NtHD-ZIP IV genes are implicated in a complex regulatory gene network controlling epidermal development and abiotic stress responses. The present study provides evidence to elucidate the gene functions of NtHD-ZIP IVs during epidermal development and stress response.

scholarly journals Evolutionary conservation of the intrinsic disorder-based Radical-Induced Cell Death1 hub interactome

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lise Friis Christensen ◽  
Lasse Staby ◽  
Katrine Bugge ◽  
Charlotte O’Shea ◽  
Birthe B. Kragelund ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Plant Stress ◽  
Three Dimensional ◽  
Intrinsic Disorder ◽  
Dimensional Structure ◽  
Linear Motif ◽  
Intrinsically Disordered ◽  
Helix Formation ◽  
Α Helix

AbstractRadical-Induced Cell Death1 (RCD1) functions as a cellular hub interacting with intrinsically disordered transcription factor regions, which lack a well-defined three-dimensional structure, to regulate plant stress. Here, we address the molecular evolution of the RCD1-interactome. Using bioinformatics, its history was traced back more than 480 million years to the emergence of land plants with the RCD1-binding short linear motif (SLiM) identified from mosses to flowering plants. SLiM variants were biophysically verified to be functional and to depend on the same RCD1 residues as the DREB2A transcription factor. Based on this, numerous additional members may be assigned to the RCD1-interactome. Conservation was further strengthened by similar intrinsic disorder profiles of the transcription factor homologs. The unique structural plasticity of the RCD1-interactome, with RCD1-binding induced α-helix formation in DREB2A, but not detectable in ANAC046 or ANAC013, is apparently conserved. Thermodynamic analysis also indicated conservation with interchangeability between Arabidopsis and soybean RCD1 and DREB2A, although with fine-tuned co-evolved binding interfaces. Interruption of conservation was observed, as moss DREB2 lacked the SLiM, likely reflecting differences in plant stress responses. This whole-interactome study uncovers principles of the evolution of SLiM:hub-interactions, such as conservation of α-helix propensities, which may be paradigmatic for disorder-based interactomes in eukaryotes.

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