scholarly journals Misidentification of Burkholderia pseudomallei and Other Burkholderia Species From Pediatric Infections in Mexico

2019 ◽  
Vol 6 (2) ◽  
Author(s):  
Georgina Meza-Radilla ◽  
Ausel Mendez-Canarios ◽  
Juan Xicohtencatl-Cortes ◽  
Marcos R Escobedo-Guerra ◽  
Alfredo G Torres ◽  
...  
Keyword(s):  
New Species ◽  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Burkholderia Cepacia Complex ◽  
Burkholderia Species ◽  
Genotypic Analysis ◽  
Pediatric Infections

Abstract Burkholderia pseudomallei and Burkholderia cepacia complex are poorly studied in Mexico. The genotypic analysis of 38 strains isolated from children with pneumonia were identified and showed that both Burkholderia groups were present in patients. From our results, it is plausible to suggest that new species are among the analyzed strains.

scholarly journals Conservation of resistance nodulation cell division efflux pump mediated antibiotic resistance in Burkholderia cepacia complex and Burkholderia pseudomallei complex species

2021 ◽  
Author(s):  
Nawarat Somprasong ◽  
Jinhee Yi ◽  
Carina M. Hall ◽  
Jessica R. Webb ◽  
Jason W. Sahl ◽  
...  
Keyword(s):  
Cell Division ◽  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Efflux Pump ◽  
Multidrug Resistant ◽  
Resistance Mechanisms ◽  
Burkholderia Cepacia Complex ◽  
Complex Species ◽  
Functional Studies ◽  
Rnd Efflux Pump

Burkholderia cepacia complex (Bcc) and Burkholderia pseudomallei complex (Bpc) species include pathogens that are typically multidrug resistant. Dominant intrinsic and acquired multidrug resistance mechanisms are efflux mediated by pumps of the resistance nodulation cell division (RND) family. From comparative bioinformatic and, in many instances, functional studies we infer that RND pump-based resistance mechanisms are conserved in Burkholderia . We propose to use these findings as a foundation for adoption of a uniform RND efflux pump nomenclature.

scholarly journals Identification of Burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody

2010 ◽  
Vol 59 (1) ◽  
pp. 41-47 ◽  
Author(s):  
David P. AuCoin ◽  
Reva B. Crump ◽  
Peter Thorkildson ◽  
Dana E. Nuti ◽  
John J. LiPuma ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Proteinase K ◽  
Burkholderia Cepacia Complex ◽  
Gram Negative Bacteria ◽  
Specific Monoclonal Antibody ◽  
Separate Species ◽  
Gram Negative ◽  
Ladder Pattern ◽  
Human Infections

The genus Burkholderia includes many bacteria that cause serious human infections. As is the case with other Gram-negative bacteria, Burkholderia species produce LPS, which is an abundant component of the bacterial cell surface. Burkholderia cepacia complex (Bcc) bacteria (which include at least 17 separate species) produce LPS structures that are quite different. In an attempt to determine the degree of LPS epitope variation among Bcc species, a mAb was produced, designated 5D8, specific for the LPS of B. cepacia. Western blot analysis determined that mAb 5D8 was able to produce the classic ‘ladder pattern’ when used to probe B. cepacia and Burkholderia anthina lysates, although 5D8 did not produce this pattern with the other seven Bcc species tested. mAb 5D8 reacted with varying intensity to most but not all of the additional B. cepacia and B. anthina strains tested. Therefore, there seems to be significant epitope variation among Bcc LPS both between and within species. Additionally, mAb 5D8 reacted with a proteinase-K-sensitive 22 kDa antigen in all Bcc strains and also in a strain of Burkholderia pseudomallei.

scholarly journals Current Advances in Burkholderia Vaccines Development

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2671
Author(s):  
Guanbo Wang ◽  
Paulina Zarodkiewicz ◽  
Miguel A. Valvano
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Vaccine Development ◽  
Respiratory Infections ◽  
Bacterial Species ◽  
Antimicrobial Treatment ◽  
Burkholderia Cepacia Complex ◽  
Attractive Alternative ◽  
Wide Range ◽  
Substantial Progress

The genus Burkholderia includes a wide range of Gram-negative bacterial species some of which are pathogenic to humans and other vertebrates. The most pathogenic species are Burkholderia mallei, Burkholderia pseudomallei, and the members of the Burkholderia cepacia complex (Bcc). B. mallei and B. pseudomallei, the cause of glanders and melioidosis, respectively, are considered potential bioweapons. The Bcc comprises a subset of Burkholderia species associated with respiratory infections in people with chronic granulomatous disease and cystic fibrosis. Antimicrobial treatment of Burkholderia infections is difficult due to the intrinsic multidrug antibiotic resistance of these bacteria; prophylactic vaccines provide an attractive alternative to counteract these infections. Although commercial vaccines against Burkholderia infections are still unavailable, substantial progress has been made over recent years in the development of vaccines against B. pseudomallei and B. mallei. This review critically discusses the current advances in vaccine development against B. mallei, B. pseudomallei, and the Bcc.

152 Delineation of five new species within the Burkholderia cepacia complex

2006 ◽  
Vol 5 ◽  
pp. S35 ◽  
Author(s):  
E. Vanlaere ◽  
A. Baldwin ◽  
E. Mahenthiralingam ◽  
C.G. Dowson ◽  
G.W. Payne ◽  
...  
Keyword(s):  
New Species ◽  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex

scholarly journals Evaluation of Three Culture Media for Isolation of Burkholderia cepacia Complex from Respiratory Samples of Patients with Cystic Fibrosis

2021 ◽  
Vol 9 (12) ◽  
pp. 2604
Author(s):  
Emma C.L. Marrs ◽  
Audrey Perry ◽  
John D. Perry
Keyword(s):  
Cystic Fibrosis ◽  
Respiratory Disease ◽  
Burkholderia Cepacia ◽  
Culture Media ◽  
The Other ◽  
Burkholderia Cepacia Complex ◽  
Becton Dickinson ◽  
Selective Media ◽  
Burkholderia Species ◽  
Selective Agar

Burkholderia cepacia complex (BCC) is a significant pathogen causing respiratory disease in individuals with cystic fibrosis (CF). Diagnosis is typically achieved by isolation of BCC on selective culture media following culture of sputum or other respiratory samples. The aim of this study was to compare the efficacy of three commercially available selective media for the isolation of BCC. The three media comprised Burkholderia cepacia selective agar (BCSA; bioMérieux), BD Cepacia medium (BD: Becton–Dickinson) and MAST Cepacia medium (MAST laboratories). Each medium was challenged with 270 respiratory samples from individuals with CF as well as an international collection of BCC (n = 26) and 14 other isolates of Burkholderia species at a range of inocula. The international collection was also used to artificially “spike” 26 respiratory samples. From a total of 34 respiratory samples containing BCC, 97% were recovered on BD and 94% were detected on MAST and BCSA. All three media were effective for isolation of BCC. BCSA was much more selective than the other two media (p < 0.0001) meaning that fewer isolates required processing to exclude the presence of BCC.

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