scholarly journals Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections

2018 ◽  
Vol 5 (6) ◽  
Author(s):  
James B Wood ◽  
Cheryl Sesler ◽  
Donald Stalons ◽  
Elena Grigorenko ◽  
Jonathan G Schoenecker ◽  
...  
Keyword(s):  
Multiplex Polymerase Chain Reaction ◽  
Pathogen Detection ◽  
Susceptibility Testing ◽  
Antimicrobial Use ◽  
Antibiotic Susceptibility Testing ◽  
Disease Course ◽  
Bacterial Identification ◽  
Pathogen Identification ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Improved diagnostics are needed for children with musculoskeletal infections (MSKIs). We assessed the performance of target-enriched multiplex polymerase chain reaction (TEM-PCR) in children with MSKI. TEM-PCR was concordant with culture in pathogen identification and antibiotic susceptibility testing, while increasing the overall yield of pathogen detection. This technology has the potential to inform judicious antimicrobial use early in the disease course.

scholarly journals Impact of Multiplex Polymerase Chain Reaction Testing for Respiratory Pathogen Detection in Pediatric Patients

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S503-S503
Author(s):  
Courtney C Sutton ◽  
Patti J Walton ◽  
Montgomery F Williams ◽  
Tracey L Bastian ◽  
Michael Wright ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Pathogen Detection ◽  
Pediatric Patients ◽  
Respiratory Pathogen ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain

scholarly journals Randomized Trial of Rapid Multiplex Polymerase Chain Reaction–Based Blood Culture Identification and Susceptibility Testing

2015 ◽  
Vol 61 (7) ◽  
pp. 1071-1080 ◽  
Author(s):  
Ritu Banerjee ◽  
Christine B. Teng ◽  
Scott A. Cunningham ◽  
Sherry M. Ihde ◽  
James M. Steckelberg ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Randomized Trial ◽  
Multiplex Polymerase Chain Reaction ◽  
Susceptibility Testing ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Culture Identification

scholarly journals Evaluation of a multiplex polymerase chain reaction for early diagnosis of ventriculostomy-related infections

2015 ◽  
Vol 123 (6) ◽  
pp. 1586-1592 ◽  
Author(s):  
Claire L. Gordon ◽  
Rafal Tokarz ◽  
Thomas Briese ◽  
W. Ian Lipkin ◽  
Komal Jain ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Pathogen Detection ◽  
High Sensitivity ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Clinical Value ◽  
Parallel Testing ◽  
Diagnostic Approaches ◽  
Polymerase Chain

OBJECT Diagnosis of ventriculostomy-related infections (VRIs) is challenging due to the lack of rapid, sensitive assays for pathogen detection. The authors report the development of a multiplex polymerase chain reaction (PCR) assay for differential diagnosis of common VRI pathogens. METHODS MassTag PCR was used to develop a multiplex assay for detection of 11 VRI pathogens. The assay was established and optimized using cloned template standards and spiked samples and was then evaluated on CSF specimens from ventricular drains. Subjects were grouped into definite VRI, possible VRI, or no VRI based on conventional microbiology, CSF evaluation, and clinical parameters. RESULTS CSF specimens were obtained from 45 subjects (median age 49 years, interquartile range 32–63 years; 51% were male). The assay detected 10–100 genome copies. It detected a pathogen in 100% (6 of 6) of definite VRI cases in which a pathogen targeted by the assay was present; these represented 67% of all definite VRIs (6 of 9). Among subjects with a possible VRI, the assay detected a pathogen in 29% (5 of 17). In subjects without overt infection the presence of a pathogen was detected in 32% of subjects (6 of 19), albeit with lower signal compared with the VRI group. CONCLUSIONS MassTag PCR enabled parallel testing of CSF specimens for 11 pathogens of VRI. The high sensitivity of PCR combined with possible device colonization, specimen contamination, and concurrent antibiotic treatments limit the clinical value of the assay, similar to other current diagnostic approaches. With further optimization, multiplex PCR may provide timely identification of multiple possible VRI pathogens and guide management, complementing classic culture approaches.

scholarly journals Multiplex polymerase chain reaction pathogen detection in patients with suspected septicemia after trauma, emergency, and burn surgery

Surgery ◽  
2012 ◽  
Vol 151 (3) ◽  
pp. 456-463 ◽  
Author(s):  
Nam K. Tran ◽  
David H. Wisner ◽  
Timothy E. Albertson ◽  
Stuart Cohen ◽  
David Greenhalgh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Pathogen Detection ◽  
Chain Reaction ◽  
Burn Surgery ◽  
Polymerase Chain

An integrated slidable and valveless microdevice with solid phase extraction, polymerase chain reaction, and immunochromatographic strip parts for multiplex colorimetric pathogen detection

Lab on a Chip ◽  
2015 ◽  
Vol 15 (21) ◽  
pp. 4148-4155 ◽  
Author(s):  
Yong Tae Kim ◽  
Dohwan Lee ◽  
Hyun Young Heo ◽  
Do Hyun Kim ◽  
Tae Seok Seo
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Phase Extraction ◽  
Pathogen Detection ◽  
Solid Phase ◽  
Phase Extraction ◽  
Pathogen Identification ◽  
Chain Reaction ◽  
Immunochromatographic Strip ◽  
Polymerase Chain ◽  
Genetic Analyzer

We developed an integrated slidable microdevice which can perform solid phase extraction, μPCR, immunochromatographic strip detection for multiplex pathogen identification on a portable genetic analyzer.

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