scholarly journals Metagenomic Sequencing of an Echovirus 30 Genome From Cerebrospinal Fluid of a Patient With Aseptic Meningitis and Orchitis

2017 ◽  
Vol 4 (3) ◽  
Author(s):  
Anne Piantadosi ◽  
Shibani S Mukerji ◽  
Pooja Chitneni ◽  
Tracey A Cho ◽  
Lisa A Cosimi ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Massively Parallel Sequencing ◽  
Wide Spectrum ◽  
Massively Parallel ◽  
Metagenomic Sequencing ◽  
Full Genome ◽  
Echovirus 30 ◽  
Viral Sequencing ◽  
Complete Viral Genome

Abstract Enteroviruses cause a wide spectrum of clinical disease. In this study, we describe the case of a young man with orchitis and aseptic meningitis who was diagnosed with enterovirus infection. Using unbiased “metagenomic” massively parallel sequencing, we assembled a near-complete viral genome, the first use of this method for full-genome viral sequencing from cerebrospinal fluid. We found that the genome belonged to the subgroup echovirus 30, which is a common cause of aseptic meningitis but has not been previously reported to cause orchitis.

scholarly journals Genomic surveillance of enterovirus associated with aseptic meningitis cases in southern Spain, 2015–2018

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fabiana Gámbaro ◽  
Ana Belén Pérez ◽  
Eduardo Agüera ◽  
Matthieu Prot ◽  
Luis Martínez-Martínez ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Southern Spain ◽  
Recombinant Form ◽  
Health Concern ◽  
Metagenomic Sequencing ◽  
Neurologic Manifestations ◽  
Public Health Concern ◽  
Echovirus 30 ◽  
Evolutionary Relatedness ◽  
Complete Genomes

AbstractNew circulating Enterovirus (EV) strains often emerge through recombination. Upsurges of recombinant non-polio enteroviruses (NPEVs) associated with neurologic manifestations such as EVA71 or Echovirus 30 (E30) are a growing public health concern in Europe. Only a few complete genomes of EVs circulating in Spain are available in public databases, making it difficult to address the emergence of recombinant EVs, understand their evolutionary relatedness and the possible implication in human disease. We have used metagenomic (untargeted) NGS to generate full-length EV genomes from CSF samples of EV-positive aseptic meningitis cases in Southern Spain between 2015 and 2018. Our analyses reveal the co-circulation of multiple Enterovirus B (EV-B) types (E6, E11, E13 and E30), including a novel E13 recombinant form. We observed a genetic turnover where emergent lineages (C1 for E6 and I [tentatively proposed in this study] for E30) replaced previous lineages circulating in Spain, some concomitant with outbreaks in other parts of Europe. Metagenomic sequencing provides an effective approach for the analysis of EV genomes directly from PCR-positive CSF samples. The detection of a novel, disease-associated, recombinant form emphasizes the importance of genomic surveillance to monitor spread and evolution of EVs.

scholarly journals Detection by PCR of Enteroviruses in Cerebrospinal Fluid during a Summer Outbreak of Aseptic Meningitis in Switzerland

1998 ◽  
Vol 36 (9) ◽  
pp. 2408-2412 ◽  
Author(s):  
Meri Gorgievski-Hrisoho ◽  
Jean-Daniel Schumacher ◽  
Nevenka Vilimonovic ◽  
Daniel Germann ◽  
Lukas Matter
Keyword(s):  
Cell Culture ◽  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Diagnostic Techniques ◽  
Rt Pcr ◽  
Echovirus 30 ◽  
Reverse Transcription Pcr ◽  
Viral Culture ◽  
Test Kit ◽  
Virus Culture

Enteroviruses (EV) are among the most common causes of aseptic meningitis. Standard diagnostic techniques are often too slow and lack sensitivity to be of clinical relevance. EV RNA can be detected within 5 h by a commercially available reverse transcription-PCR (RT-PCR) test kit. Cerebrospinal fluid (CSF) samples from 68 patients presenting with aseptic meningitis during a summer outbreak in Switzerland were examined in parallel with cell culture and commercial RT-PCR. RT-PCR was positive in all 16 CSF specimens positive by cell culture (100%). In addition, 42 of 52 (80%) CSF samples negative by cell culture were PCR positive. In 26 of these 42 (62%) patients, viral culture from other sites (throat swab or stool) was also positive. The CSF virus culture took 3 to 7 days to become positive. Echovirus 30 was the type most often isolated in this outbreak. The sensitivity of CSF RT-PCR based on clinical diagnosis during this aseptic meningitis outbreak in patients with negative bacterial culture results was 85%, i.e., considerably higher than the sensitivity of CSF virus culture (24%). We conclude that this commercial RT-PCR assay allows a positive diagnosis with minimal delay and may thus influence clinical decisions.

scholarly journals Nearly Complete Genome Sequence of an Echovirus 30 Strain from a Cluster of Aseptic Meningitis Cases in California, September 2017

2019 ◽  
Vol 8 (44) ◽  
Author(s):  
Chao-Yang Pan ◽  
Thalia Huynh ◽  
Tasha Padilla ◽  
Alice Chen ◽  
Terry Fei Fan Ng ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Human Enterovirus ◽  
Echovirus 30

We report the nearly complete genome sequence of a human enterovirus, a strain of echovirus 30, obtained from a cerebrospinal fluid specimen from a teenaged patient with aseptic meningitis in September 2017.

scholarly journals Identification of the Bovine Arachnomelia Mutation by Massively Parallel Sequencing Implicates Sulfite Oxidase (SUOX) in Bone Development

PLoS Genetics ◽  
2010 ◽  
Vol 6 (8) ◽  
pp. e1001079 ◽  
Author(s):  
Cord Drögemüller ◽  
Jens Tetens ◽  
Snaevar Sigurdsson ◽  
Arcangelo Gentile ◽  
Stefania Testoni ◽  
...  
Keyword(s):  
Massively Parallel Sequencing ◽  
Bone Development ◽  
Sulfite Oxidase ◽  
Massively Parallel ◽  
Parallel Sequencing
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