scholarly journals Multisite Laboratory Evaluation of a Dual Human Immunodeficiency Virus (HIV)/Syphilis Point-of-Care Rapid Test for Simultaneous Detection of HIV and Syphilis Infection

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
Claire C. Bristow ◽  
Yaw Adu-Sarkodie ◽  
Raphael O. Ondondo ◽  
Elizabeth Anne Bukusi ◽  
Claver Anoumou Dagnra ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Sensitivity And Specificity ◽  
Solid Phase ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Laboratory Evaluation ◽  
Test Results ◽  
Immunodeficiency Virus ◽  
Test Component ◽  
Rapid Tests

Abstract Background.  Recently, test developers have created rapid point-of-care tests that can simultaneously detect multiple infections within the same specimen using a single device. The SD BIOLINE Duo HIV/Syphilis rapid point-of-care test uses a solid-phase immunochromatographic assay to detect immunoglobulin (Ig)G, IgM, and IgA antibodies to human immunodeficiency virus (HIV)-specific antigens (HIV-1 gp41, sub O, HIV-2 gp36) and recombinant Treponema pallidum antigen (17 kDa) in human serum. This study was a multisite laboratory-based evaluation of the performance of SD BIOLINE HIV/Syphilis Duo test using previously characterized sera in 6 countries. Methods.  Laboratories in Ghana, Mexico, Laos, Togo, Kenya, and Myanmar participated in the evaluation during 2012–2013. Each site characterized sera using T pallidum particle agglutination assay or T pallidum hemagglutination assay and HIV enzyme immunoassay, Western blot, and/or HIV antibody rapid tests. Those gold standard test results were compared with SD BIOLINE Duo test results. We calculated the sensitivity and specificity of test performance and used the exact binomial method to calculate 95% confidence intervals (CIs). Results.  The sensitivity and specificity for the HIV antibody test component (n = 2336) were estimated at 99.91% (95% CI, 99.51% and 100%) and 99.67% (95% CI, 99.16% and 99.91%), respectively. For the T pallidum test component (n = 2059), the sensitivity and specificity were estimated at 99.67% (95% CI, 98.82% and 99.96%) and 99.72% (95% CI, 99.29% and 99.92%), respectively. Conclusions.  The sensitivity and specificity of the SD BIOLINE HIV/Syphilis Duo test were consistently high across sera specimens from 6 countries around the world. Dual rapid tests should be considered for improved HIV and syphilis screening coverage.

scholarly journals Diagnosis of Human Immunodeficiency Virus Type 1 Infection with Different Subtypes Using Rapid Tests

2000 ◽  
Vol 7 (4) ◽  
pp. 698-699 ◽  
Author(s):  
Susan Phillips ◽  
Timothy C. Granade ◽  
Chou-Pong Pau ◽  
Debra Candal ◽  
Dale J. Hu ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Sensitivity And Specificity ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Local Population ◽  
Rapid Test ◽  
Immunodeficiency Virus ◽  
Rapid Tests ◽  
Hiv 1

ABSTRACT We evaluated six rapid tests for their sensitivity and specificity in diagnosing human immunodeficiency virus type 1 (HIV-1) infection using 241 specimens (172 HIV-1 positive, 69 HIV-1 negative) representing different HIV-1 subtypes (A [n = 40], B [n = 47], C [n = 28], E [n = 42], and F [n = 7]). HIVCHEK, Multispot, RTD and SeroStrip were 100% sensitive and specific. Capillus failed to identify two of eight subtype C specimens (overall sensitivity of 98.85%), while the SUDS test (the only test approved by the Food and Drug Administration) gave false-positive results for 5 of 69 seronegative specimens (specificity of 93.24%). Our results suggest that although rapid tests perform well in general, it may be prudent to evaluate a rapid test for sensitivity and specificity in a local population prior to its widespread use.

scholarly journals Evaluation of Rapid Prenatal Human Immunodeficiency Virus Testing in Rural Cameroon

2005 ◽  
Vol 12 (7) ◽  
pp. 855-860 ◽  
Author(s):  
Timothy C. Granade ◽  
Bharat S. Parekh ◽  
Pius M. Tih ◽  
Thomas Welty ◽  
Edith Welty ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Sensitivity And Specificity ◽  
Field Performance ◽  
Human Immunodeficiency Virus Testing ◽  
Dried Blood Spots ◽  
Testing Strategies ◽  
Immunodeficiency Virus ◽  
Rural Cameroon ◽  
Rapid Tests ◽  
Testing Algorithms

ABSTRACT Pregnant women (n = 859) in rural Cameroonian prenatal clinics were screened by two rapid human immunodeficiency virus (HIV) antibody tests (rapid tests [RT]) (Determine and Hema-Strip) using either whole blood or plasma. One additional RT (Capillus, HIV-CHEK, or Sero-Card) was used to resolve discordant results. RT results were compared with HIV-1 enzyme immunoassay (EIA) and Western blot (WB) results of matched dried blood spots (DBS) to assess the accuracy of HIV RTs. DBS EIA/WB identified 83 HIV antibody-reactive, 763 HIV antibody-nonreactive, and 13 indeterminate specimens. RT results were evaluated in serial (two consecutive tests) or parallel (two simultaneous tests) testing algorithms. A serial algorithm using Determine and Hema-Strip yielded sensitivity and specificity results of 97.6% and 99.7%, respectively, whereas a parallel RT algorithm using Determine plus a second RT produced a sensitivity and specificity of 100% and 99.7%, respectively. HIV RTs provide excellent alternatives for identifying HIV infection, and their field performance could be monitored using DBS testing strategies.

scholarly journals Current Status of Point-of-Care Testing for Human Immunodeficiency Virus Drug Resistance

2017 ◽  
Vol 216 (suppl_9) ◽  
pp. S824-S828 ◽  
Author(s):  
Horacio A Duarte ◽  
Nuttada Panpradist ◽  
Ingrid A Beck ◽  
Barry Lutz ◽  
James Lai ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Drug Resistance ◽  
Point Of Care ◽  
Current Status ◽  
Point Of Care Testing ◽  
Immunodeficiency Virus

scholarly journals Quantitation of Intracellular Triphosphate of Emtricitabine in Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Patients

1999 ◽  
Vol 43 (9) ◽  
pp. 2245-2250 ◽  
Author(s):  
Albert Darque ◽  
Gilles Valette ◽  
Frank Rousseau ◽  
Laurene H. Wang ◽  
Jean-Pierre Sommadossi ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Anion Exchange ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Solid Phase ◽  
Peripheral Blood Mononuclear ◽  
Limit Of Quantitation ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells

ABSTRACT An analytical methodology combining solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) was developed to quantitate the intracellular active 5′-triphosphate (TP) of β-l-2′,3′-dideoxy-5-fluoro-3′-thiacytidine (emtricitabine) (FTC) in human peripheral blood mononuclear cells (PBMCs). The FTC nucleotides, including 5′-mono-, di-, and triphosphates, were successively resolved on an anion-exchange SPE cartridge by applying a gradient of potassium chloride. The FTC-TP was subsequently digested to release the parent nucleoside that was finally analyzed by HPLC with UV detection (HPLC-UV). Validation of the methodology was performed by using PBMCs from healthy donors exposed to an isotopic solution of [3H]FTC with known specific activity, leading to the formation of intracellular FTC-TP that was quantitated by an anion-exchange HPLC method with radioactive detection. These levels of FTC-TP served as reference values and were used to validate the data obtained by HPLC-UV. The assay had a limit of quantitation of 4.0 pmol of FTC-TP (amount on column from approximately 107 cells). Intra-assay precision (coefficient of variation percentage of repeated measurement) and accuracy (percentage deviation of the nominal reference value), estimated by using quality control samples at 16.2, 60.7, and 121.5 pmol, ranged from 1.3 to 3.3% and −1.0 to 4.8%, respectively. Interassay precision and accuracy varied from 3.0 to 10.2% and from 2.5 to 6.7%, respectively. This methodology was successfully applied to the determination of FTC-TP in PBMCs of patients infected with human immunodeficiency virus after oral administration of various dosing regimens of FTC monotherapy.

scholarly journals Field Evaluation of the Merlin Immediate HIV-1 and -2 Test for Point-of-Care Detection of Human Immunodeficiency Virus Antibodies

2002 ◽  
Vol 34 (5) ◽  
pp. 658-661 ◽  
Author(s):  
S. D. Shafran ◽  
B. Conway ◽  
E. Prasad ◽  
J. Greer ◽  
J. Vincelette ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Point Of Care ◽  
Field Evaluation ◽  
Immunodeficiency Virus ◽  
Point Of Care Detection ◽  
Hiv 1

scholarly journals “SILVAMP TB LAM” Rapid Urine Tuberculosis Test Predicts Mortality in Patients Hospitalized With Human Immunodeficiency Virus in South Africa

2020 ◽  
Vol 71 (8) ◽  
pp. 1973-1976 ◽  
Author(s):  
Bianca Sossen ◽  
Tobias Broger ◽  
Andrew D Kerkhoff ◽  
Charlotte Schutz ◽  
Andre Trollip ◽  
...  
Keyword(s):  
South Africa ◽  
Human Immunodeficiency Virus ◽  
Point Of Care ◽  
Diagnostic Delay ◽  
Tuberculosis Patients ◽  
Immunodeficiency Virus ◽  
Urine Testing

Abstract Reducing diagnostic delay is key toward decreasing tuberculosis-associated deaths in people living with human immunodeficiency virus. In tuberculosis patients with retrospective urine testing, the point-of-care Fujifilm SILVAMP TB LAM (FujiLAM) could have rapidly diagnosed tuberculosis in up to 89% who died. In FujiLAM negative patients, the probability of 12-week survival was 86–97%.

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