Kidney injury molecule-1 staining in renal allograft biopsies 10 days after transplantation is inversely correlated with functioning proximal tubular epithelial cells

Introduction: LincRNA-p21 is predicted to interact with miR-449a, which plays a protective role in cisplatin-induced acute kidney injury (CIA). Objective: This study aimed to analyze the involvement of lincRNA-p21 in breast cancer patients with CIA. Methods: Levels of lincRNA-p21 in plasma from CIA, triple negative breast cancer, and control groups were measured by performing RT-qPCR. The potential interaction between lincRNA-p21 and miR-449a was first predicted by RT-qPCR. The relationship between lincRNA-p21 and miR-449a was analyzed by overexpression experiment. Results: We found that lincRNA-p21 is downregulated in CIA. Dual luciferase activity assay showed that lincRNA-p21 and miR-449a can interact with each other, while overexpression of lincRNA-p21 and miR-449a failed to affect the expression of each other. In human renal proximal tubular epithelial cells (HRPTEpCs), cisplatin led to the upregulated miR-449a but downregulated lincRNA-p21. Interestingly, lincRNA-p21 overexpression led to reduced enhancing effects of miR-449a on the cisplatin-induced apoptosis of HRPTEpCs. Conclusion: Therefore, lincRNA-p21 is downregulated in CIA and may sponge miR-449a to inhibit cisplatin-induced apoptosis of HRPTEpCs.

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Proximal tubule-specific overexpression of netrin-1 suppresses acute kidney injury-induced interstitial fibrosis and glomerulosclerosis through suppression of IL-6/STAT3 signaling

AJP Renal Physiology ◽

10.1152/ajprenal.00650.2012 ◽

2013 ◽

Vol 304 (8) ◽

pp. F1054-F1065 ◽

Cited By ~ 22

Author(s):

Punithavathi Ranganathan ◽

Calpurnia Jayakumar ◽

Ganesan Ramesh

Keyword(s):

Acute Kidney Injury ◽

Epithelial Cells ◽

Interstitial Fibrosis ◽

Kidney Injury ◽

Transgenic Animals ◽

Acute Injury ◽

Tubular Epithelial Cells ◽

Wild Type ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular

Acute kidney injury-induced organ fibrosis is recognized as a major risk factor for the development of chronic kidney disease, which remains one of the leading causes of death in the developed world. However, knowledge on molecules that may suppress the fibrogenic response after injury is lacking. In ischemic models of acute kidney injury, we demonstrate a new function of netrin-1 in regulating interstitial fibrosis. Acute injury was promptly followed by a rise in serum creatinine in both wild-type and netrin-1 transgenic animals. However, the wild-type showed a slow recovery of kidney function compared with netrin-1 transgenic animals and reached baseline by 3 wk. Histological examination showed increased infiltration of interstitial macrophages, extensive fibrosis, reduction of capillary density, and glomerulosclerosis. Collagen IV and α-smooth muscle actin expression was absent in sham-operated kidneys; however, their expression was significantly increased at 2 wk and peaked at 3 wk after reperfusion. These changes were reduced in the transgenic mouse kidney, which overexpresses netrin-1 in proximal tubular epithelial cells. Fibrosis was associated with increased expression of IL-6 and extensive and chronic activation of STAT3. Administration of IL-6 exacerbated fibrosis in vivo in wild-type, but not in netrin-1 transgenic mice kidney and increased collagen I expression and STAT3 activation in vitro in renal epithelial cells subjected to hypoxia-reoxygenation, which was suppressed by netrin-1. Our data suggest that proximal tubular epithelial cells may play a prominent role in interstitial fibrosis and that netrin-1 could be a useful therapeutic agent for treating kidney fibrosis.

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TCR+CD4−CD8− (double negative) T cells protect from cisplatin-induced renal epithelial cell apoptosis and acute kidney injury

AJP Renal Physiology ◽

10.1152/ajprenal.00033.2020 ◽

2020 ◽

Vol 318 (6) ◽

pp. F1500-F1512

Author(s):

Jing Gong ◽

Sanjeev Noel ◽

Joshua Hsu ◽

Errol L. Bush ◽

Lois J. Arend ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Epithelial Cells ◽

Kidney Injury ◽

Double Negative ◽

Tubular Epithelial Cells ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular ◽

Induced Apoptosis

Acute kidney injury (AKI) due to cisplatin is a significant problem that limits its use as an effective chemotherapeutic agent. T cell receptor+CD4−CD8− double negative (DN) T cells constitute the major T cell population in the human and mouse kidney, express programmed cell death protein (PD)-1, and protect from ischemic AKI. However, the pathophysiological roles of DN T cells in cisplatin-induced AKI is unknown. In this study, wild-type mice were treated with cisplatin (30 mg/kg) or vehicle, and the effects on kidney DN T cell numbers and function were measured. In vitro experiments evaluated effects of kidney DN T cells on cisplatin-induced apoptosis and PD ligand 1 (PD-L1) in renal epithelial cells. Adoptive transfer experiments assessed the therapeutic potential of DN T cells during cisplatin-induced AKI. Our results show that kidney DN T cell population increased at 24 h and declined by 72 h after cisplatin treatment. Cisplatin treatment increased kidney DN T cell proliferation, apoptosis, CD69, and IL-10 expression, whereas CD62L, CD44, IL-17A, interferon-γ, and TNF-α were downregulated. Cisplatin treatment decreased both PD-1 and natural killer 1.1 subsets of kidney DN T cells with a pronounced effect on the PD-1 subset. In vitro kidney DN T cell coculture decreased cisplatin-induced apoptosis in kidney proximal tubular epithelial cells, increased Bcl-2, and decreased cleaved caspase 3 expression. Cisplatin-induced expression of PD ligand 1 was reduced in proximal tubular epithelial cells cocultured with DN T cells. Adoptive transfer of DN T cells attenuated kidney dysfunction and structural damage from cisplatin-induced AKI. These results demonstrate that kidney DN T cells respond rapidly and play a protective role during cisplatin-induced AKI.

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DONOR-SPECIFIC LYSIS OF HUMAN KIDNEY PROXIMAL TUBULAR EPITHELIAL CELLS BY RENAL ALLOGRAFT-INFILTRATING LYMPHOCYTES

Transplantation Journal ◽

10.1097/00007890-198908000-00022 ◽

1989 ◽

Vol 48 (2) ◽

pp. 296-301 ◽

Cited By ~ 53

Author(s):

A. M. M. MILTENBURG ◽

M. E. MEIJER-PAAPE ◽

M. R. DAHA ◽

J. H. VAN BOCKEL ◽

J. J. WEENING ◽

...

Keyword(s):

Epithelial Cells ◽

Renal Allograft ◽

Human Kidney ◽

Tubular Epithelial Cells ◽

Specific Lysis ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular ◽

Infiltrating Lymphocytes

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Edaravone-Loaded Mesoscale Nanoparticles Treat Cisplatin-Induced Acute Kidney Injury

10.1101/2020.01.24.919134 ◽

2020 ◽

Author(s):

Ryan M. Williams ◽

Janki Shah ◽

Elizabeth Mercer ◽

Helen S. Tian ◽

Justin M. Cheung ◽

...

Keyword(s):

Oxidative Stress ◽

Acute Kidney Injury ◽

Epithelial Cells ◽

Kidney Injury ◽

Tubular Epithelial Cells ◽

Co Morbidity ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular ◽

Tumor Accumulation ◽

Renal Proximal Tubular

AbstractCisplatin-induced acute kidney injury (CI-AKI) is a significant co-morbidity of chemotherapeutic regimens. While this condition is associated with substantially lower survival and increased economic burden, there is no pharmacological agent to effectively treat CI-AKI. The disease is hallmarked by acute tubular necrosis of the proximal tubular epithelial cells primarily due to increased oxidative stress. In our prior work, we developed a highly-selective kidney-targeted mesoscale nanoparticle (MNP) that accumulates primarily in the renal proximal tubular epithelial cells while exhibiting no toxicity. Here, we found that MNPs exhibit renal-selective targeting in multiple mouse models of tumor growth with virtually no tumor accumulation. We then evaluated the therapeutic efficacy of MNPs loaded with the reactive oxygen species scavenger edaravone in a mouse model of CI-AKI. We found a marked and significant therapeutic effect with this approach as compared to free drug or empty control MNPs, including improved renal function, histology, and diminution of oxidative stress. These results indicated that renal-selective MNP edaravone delivery holds substantial potential in the treatment of acute kidney injury among patients undergoing cisplatin-based chemotherapy.

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Lithium targeting of AMPK protects against cisplatin-induced acute kidney injury by enhancing autophagy in renal proximal tubular epithelial cells

The FASEB Journal ◽

10.1096/fj.201901712r ◽

2019 ◽

Vol 33 (12) ◽

pp. 14370-14381 ◽

Cited By ~ 6

Author(s):

Hui Bao ◽

Qianyun Zhang ◽

Xinying Liu ◽

Yaxiang Song ◽

Xinhua Li ◽

...

Keyword(s):

Acute Kidney Injury ◽

Epithelial Cells ◽

Kidney Injury ◽

Tubular Epithelial Cells ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular ◽

Renal Proximal Tubular

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Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Proximal Tubular Epithelial Cells through miR-545-3p–TNFSF10

Biomolecules ◽

10.3390/biom11071032 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1032

Author(s):

Mei-Chuan Kuo ◽

Wei-An Chang ◽

Ling-Yu Wu ◽

Yi-Chun Tsai ◽

Ya-Ling Hsu

Keyword(s):

Epithelial Cells ◽

Epithelial To Mesenchymal Transition ◽

Kidney Injury ◽

Tubular Epithelial Cells ◽

Kidney Fibrosis ◽

Mesenchymal Transition ◽

Proximal Tubular Epithelial Cells ◽

Proximal Tubular ◽

Critical Process ◽

Necrosis Factor

Hypoxia is regarded as one of the pathophysiologic mechanisms of kidney injury and further progression to kidney failure. Epithelial-to-mesenchymal transition (EMT) in kidney tubules is a critical process of kidney fibrosis. This study utilized transcriptome analysis to investigate hypoxia-induced EMT through microRNA (miRNA)-modulated EMT in proximal tubular epithelial cells (PTECs). RNA sequencing revealed eight miRNAs were upregulated and three miRNAs were downregulated in PTECs cultured under hypoxia compared with normoxia. Among the 11 miRNAs, miR-545-3p has the highest expression in PTECs exposed to hypoxia, and miR-545-3p suppressed tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/TNFSF10) expression. Hypoxia induced EMT in PTECs through miR-545-3p–TNFSF10 modulation, and TNFSF10-attenuated EMT resulted from hypoxia or miR-545-3p mimic transfection. These findings provided new perceptions of the unique regulation of the miR-545-3p–TNFSF10 interaction and their potential therapeutic effect in kidney injury induced by hypoxia.

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