scholarly journals Inducible model for  -six-mediated site-specific recombination in mammalian cells

2006 ◽  
Vol 34 (1) ◽  
pp. e1-e1 ◽  
Author(s):  
P. Servert
Keyword(s):  
Mammalian Cells ◽  
Site Specific ◽  
Site Specific Recombination

A diversity of serine phage integrases mediate site-specific recombination in mammalian cells

2006 ◽  
Vol 276 (2) ◽  
Author(s):  
Annahita Keravala ◽  
Amy C. Groth ◽  
Sohail Jarrahian ◽  
Bhaskar Thyagarajan ◽  
Jason J. Hoyt ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Phage Integrases

scholarly journals The Prokaryotic β-Recombinase Catalyzes Site-specific Recombination in Mammalian Cells

1999 ◽  
Vol 274 (10) ◽  
pp. 6634-6640 ◽  
Author(s):  
Vicente Dı́az ◽  
Fernando Rojo ◽  
Carlos Martínez-A ◽  
Juan C. Alonso ◽  
Antonio Bernad
Keyword(s):  
Mammalian Cells ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells

BioTechniques ◽  
2006 ◽  
Vol 40 (4) ◽  
pp. 460-464 ◽  
Author(s):  
John P. Russell ◽  
David W. Chang ◽  
Anna Tretiakova ◽  
Malla Padidam
Keyword(s):  
Mammalian Cells ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Highly Efficient

scholarly journals Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1

2007 ◽  
Vol 36 (1) ◽  
pp. e9-e9 ◽  
Author(s):  
Zhengyao Xu ◽  
Nicholas C. O. Lee ◽  
Felix Dafhnis-Calas ◽  
Sunir Malla ◽  
Margaret C. M. Smith ◽  
...  
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Mammalian Cells ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells

PLoS ONE ◽  
2007 ◽  
Vol 2 (1) ◽  
pp. e162 ◽  
Author(s):  
Christopher S. Raymond ◽  
Philippe Soriano
Keyword(s):  
Mammalian Cells ◽  
High Efficiency ◽  
Site Specific ◽  
Site Specific Recombination

scholarly journals Positive selection of FLP-mediated unequal sister chromatid exchange products in mammalian cells.

1997 ◽  
Vol 17 (2) ◽  
pp. 857-861 ◽  
Author(s):  
M I Aladjem ◽  
L L Brody ◽  
S O'Gorman ◽  
G M Wahl
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Mammalian Cells ◽  
Gene Dosage ◽  
Direct Selection ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Flp Recombinase ◽  
Screening Strategies ◽  
Target Sites

Site-specific recombination provides a powerful tool for studying gene function at predetermined chromosomal sites. Here we describe the use of a blasticidin resistance system to select for recombination in mammalian cells using the yeast enzyme FLP. The vector is designed so that site-specific recombination reconstructs the antibiotic resistance marker within the sequences flanked by the FLP target sites. This approach allows the detection of DNA excised by FLP-mediated recombination and facilitates the recovery of recombination products that would not be detected by available screening strategies. We used this system to show that the molecules excised by intrachromosomal recombination between tandem FLP recombinase target sites do not reintegrate into the host genome at detectable frequencies. We further applied the direct selection approach to recover a rare FLP-mediated recombination event displaying the characteristics of an unequal sister chromatid exchange between FLP target sites. Implications of this approach for the generation of duplications to assess their effect on gene dosage and chromosome stability are discussed.

Sign in / Sign up

Export Citation Format

Share Document