scholarly journals CellAtlasSearch: a scalable search engine for single cells

2018 ◽  
Vol 46 (W1) ◽  
pp. W141-W147 ◽  
Author(s):  
Divyanshu Srivastava ◽  
Arvind Iyer ◽  
Vibhor Kumar ◽  
Debarka Sengupta
Keyword(s):  
Search Engine ◽  
Single Cells ◽  
Scalable Search

scholarly journals An efficient and scalable search engine for models

2021 ◽  
Author(s):  
José Antonio Hernández López ◽  
Jesús Sánchez Cuadrado
Keyword(s):  
Search Engine ◽  
Search Engines ◽  
Response Times ◽  
Fast Response ◽  
Text Search ◽  
New Developments ◽  
Data Analyses ◽  
Search Precision ◽  
Scalable Search ◽  
Uml Models

AbstractSearch engines extract data from relevant sources and make them available to users via queries. A search engine typically crawls the web to gather data, analyses and indexes it and provides some query mechanism to obtain ranked results. There exist search engines for websites, images, code, etc., but the specific properties required to build a search engine for models have not been explored much. In the previous work, we presented MAR, a search engine for models which has been designed to support a query-by-example mechanism with fast response times and improved precision over simple text search engines. The goal of MAR is to assist developers in the task of finding relevant models. In this paper, we report new developments of MAR which are aimed at making it a useful and stable resource for the community. We present the crawling and analysis architecture with which we have processed about 600,000 models. The indexing process is now incremental and a new index for keyword-based search has been added. We have also added a web user interface intended to facilitate writing queries and exploring the results. Finally, we have evaluated the indexing times, the response time and search precision using different configurations. MAR has currently indexed over 500,000 valid models of different kinds, including Ecore meta-models, BPMN diagrams, UML models and Petri nets. MAR is available at http://mar-search.org.

scholarly journals A scalable search engine for mass storage smart objects

2015 ◽  
Vol 8 (9) ◽  
pp. 910-921 ◽  
Author(s):  
Nicolas Anciaux ◽  
Saliha Lallali ◽  
Iulian Sandu Popa ◽  
Philippe Pucheral
Keyword(s):  
Search Engine ◽  
Mass Storage ◽  
Smart Objects ◽  
Scalable Search

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

Selective Staining of Acid Mucopolysaccharides By Ruthenium Red

1968 ◽  
Vol 26 ◽  
pp. 38-39
Author(s):  
J. H. Luft
Keyword(s):  
Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Single Cells ◽  
Ruthenium Red ◽  
Collagen Fibers ◽  
Selective Staining ◽  
Pectic Substances ◽  
Solid Tissues

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

Who owns a personal home page?

2003 ◽  
Vol 62 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Astrid Schütz ◽  
Franz Machilek
Keyword(s):  
Search Engine ◽  
Home Page ◽  
Sampling Strategies ◽  
Home Pages ◽  
Personal Home Pages ◽  
Age And Sex

Research on personal home pages is still rare. Many studies to date are exploratory, and the problem of drawing a sample that reflects the variety of existing home pages has not yet been solved. The present paper discusses sampling strategies and suggests a strategy based on the results retrieved by a search engine. This approach is used to draw a sample of 229 personal home pages that portray private identities. Findings on age and sex of the owners and elements characterizing the sites are reported.

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