scholarly journals Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis

2018 ◽  
Vol 46 (10) ◽  
pp. e63-e63
Author(s):  
Ayako Yamanishi ◽  
Atsushi Matsuba ◽  
Ryohei Kondo ◽  
Rie Akamatsu ◽  
Sachiyo Tanaka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Trap ◽  
Homozygous Mutant ◽  
Homozygous Mutant Mouse

scholarly journals Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system

2011 ◽  
Vol 40 (3) ◽  
pp. e21-e21 ◽  
Author(s):  
Yue Huang ◽  
Stephen J. Pettitt ◽  
Ge Guo ◽  
Guang Liu ◽  
Meng Amy Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Selection System ◽  
Homozygous Mutant ◽  
Homozygous Mutant Mouse

scholarly journals An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers

2017 ◽  
Vol 45 (21) ◽  
pp. e174-e174 ◽  
Author(s):  
Cynthia L. Fisher ◽  
Hendrik Marks ◽  
Lily Ting-yin Cho ◽  
Robert Andrews ◽  
Sam Wormald ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Efficient Method ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Null Mutant ◽  
Epigenetic Modifiers ◽  
Null Mutant Mouse

scholarly journals Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiaona Liu ◽  
Hailong Wang ◽  
Xueya Zhao ◽  
Qizhi Luo ◽  
Qingwen Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Arginine Methylation ◽  
Mouse Embryonic Stem Cells ◽  
Protein Methylation ◽  
Dynamic Regulation ◽  
Endoderm Differentiation

AbstractRNA N6-methyladenosine (m6A), the most abundant internal modification of mRNAs, plays key roles in human development and health. Post-translational methylation of proteins is often critical for the dynamic regulation of enzymatic activity. However, the role of methylation of the core methyltransferase METTL3/METTL14 in m6A regulation remains elusive. We find by mass spectrometry that METTL14 arginine 255 (R255) is methylated (R255me). Global mRNA m6A levels are greatly decreased in METTL14 R255K mutant mouse embryonic stem cells (mESCs). We further find that R255me greatly enhances the interaction of METTL3/METTL14 with WTAP and promotes the binding of the complex to substrate RNA. We show that protein arginine N-methyltransferases 1 (PRMT1) interacts with and methylates METTL14 at R255, and consistent with this, loss of PRMT1 reduces mRNA m6A modification globally. Lastly, we find that loss of R255me preferentially affects endoderm differentiation in mESCs. Collectively, our findings show that arginine methylation of METTL14 stabilizes the binding of the m6A methyltransferase complex to its substrate RNA, thereby promoting global m6A modification and mESC endoderm differentiation. This work highlights the crosstalk between protein methylation and RNA methylation in gene expression.

scholarly journals The phenotype of FancB-mutant mouse embryonic stem cells

2011 ◽  
Vol 712 (1-2) ◽  
pp. 20-27 ◽  
Author(s):  
Tae Moon Kim ◽  
Jun Ho Ko ◽  
Yong Jun Choi ◽  
Lingchuan Hu ◽  
Paul Hasty
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Functional Genomics Screening Utilizing Mutant Mouse Embryonic Stem Cells Identifies Novel Radiation-Response Genes

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0120534 ◽  
Author(s):  
Kimberly Loesch ◽  
Stacy Galaviz ◽  
Zaher Hamoui ◽  
Ryan Clanton ◽  
Gamal Akabani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Functional Genomics ◽  
Mutant Mouse ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Radiation Response
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