scholarly journals ‘Modulation of the enzymatic activities of replicative helicase (DnaB) by interaction with Hp0897: a possible mechanism for helicase loading inHelicobacter pylori’

2016 ◽  
Vol 44 (7) ◽  
pp. 3288-3303 ◽  
Author(s):  
Vijay Verma ◽  
Ajay Kumar ◽  
Ram Gopal Nitharwal ◽  
Jawed Alam ◽  
Asish Kumar Mukhopadhyay ◽  
...  
Keyword(s):  
Enzymatic Activities ◽  
Replicative Helicase ◽  
Helicase Loading ◽  
Helicase Dnab

scholarly journals The molecular coupling between substrate recognition and ATP turnover in a AAA+ hexameric helicase loader

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Neha Puri ◽  
Amy J Fernandez ◽  
Valerie L O'Shea Murray ◽  
Sarah McMillan ◽  
James L Keck ◽  
...  
Keyword(s):  
Replication Fork ◽  
Substrate Recognition ◽  
Dna Unwinding ◽  
Replicative Helicase ◽  
E Coli ◽  
Helicase Loading ◽  
Dnab Helicase ◽  
Replication Restart ◽  
Clamp Loading ◽  
Aaa Atpases

In many bacteria and in eukaryotes, replication fork establishment requires the controlled loading of hexameric, ring-shaped helicases around DNA by AAA+ ATPases. How loading factors use ATP to control helicase deposition is poorly understood. Here, we dissect how specific ATPase elements of E. coli DnaC, an archetypal loader for the bacterial DnaB helicase, play distinct roles in helicase loading and the activation of DNA unwinding. We identify a new element, the arginine-coupler, which regulates the switch-like behavior of DnaC to prevent futile ATPase cycling and maintains loader responsiveness to replication restart systems. Our data help explain how the ATPase cycle of a AAA+-family helicase loader is channeled into productive action on its target; comparative studies indicate elements analogous to the Arg-coupler are present in related, switch-like AAA+ proteins that control replicative helicase loading in eukaryotes, as well as polymerase clamp loading and certain classes of DNA transposases.

Strand Specificity in the Interactions ofEscherichia coliPrimary Replicative Helicase DnaB Protein with a Replication Fork†

Biochemistry ◽  
1997 ◽  
Vol 36 (33) ◽  
pp. 10320-10326 ◽  
Author(s):  
Maria J. Jezewska ◽  
Surendran Rajendran ◽  
Wlodzimierz Bujalowski
Keyword(s):  
Replication Fork ◽  
Replicative Helicase ◽  
Helicase Dnab
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