scholarly journals ECHO-liveFISH:in vivoRNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues

2015 ◽  
Vol 43 (19) ◽  
pp. e126-e126 ◽  
Author(s):  
Ikumi Oomoto ◽  
Asuka Suzuki-Hirano ◽  
Hiroki Umeshima ◽  
Yong-Woon Han ◽  
Hiroyuki Yanagisawa ◽  
...  
Keyword(s):  
Dynamic Regulation ◽  
Rna Foci ◽  
Nuclear Rna ◽  
Living Tissues

scholarly journals Dynamic changes of nuclear RNA foci in proliferating DM1 cells

2015 ◽  
Vol 143 (6) ◽  
pp. 557-564 ◽  
Author(s):  
Guangbin Xia ◽  
Tetsuo Ashizawa
Keyword(s):  
Dynamic Changes ◽  
Rna Foci ◽  
Nuclear Rna

scholarly journals Genome Editing of Expanded CTG Repeats within the Human DMPK Gene Reduces Nuclear RNA Foci in the Muscle of DM1 Mice

2019 ◽  
Vol 27 (8) ◽  
pp. 1372-1388 ◽  
Author(s):  
Mirella Lo Scrudato ◽  
Karine Poulard ◽  
Célia Sourd ◽  
Stéphanie Tomé ◽  
Arnaud F. Klein ◽  
...  
Keyword(s):  
Genome Editing ◽  
Ctg Repeats ◽  
Rna Foci ◽  
Nuclear Rna ◽  
Dmpk Gene

scholarly journals Antisense transcripts of the expanded C9ORF72 hexanucleotide repeat form nuclear RNA foci and undergo repeat-associated non-ATG translation in c9FTD/ALS

2013 ◽  
Vol 126 (6) ◽  
pp. 829-844 ◽  
Author(s):  
Tania F. Gendron ◽  
Kevin F. Bieniek ◽  
Yong-Jie Zhang ◽  
Karen Jansen-West ◽  
Peter E. A. Ash ◽  
...  
Keyword(s):  
Antisense Transcripts ◽  
Hexanucleotide Repeat ◽  
Rna Foci ◽  
Nuclear Rna

scholarly journals Nuclear RNA Foci in the Heart in Myotonic Dystrophy

2005 ◽  
Vol 97 (11) ◽  
pp. 1152-1155 ◽  
Author(s):  
Ami Mankodi ◽  
Xiaoyan Lin ◽  
Burns C. Blaxall ◽  
Maurice S. Swanson ◽  
Charles A. Thornton
Keyword(s):  
Myotonic Dystrophy ◽  
Rna Foci ◽  
Nuclear Rna

scholarly journals Nuclear RNA foci from C9ORF72 expansion mutation form paraspeckle-like bodies

2019 ◽  
Vol 132 (5) ◽  
pp. jcs224303 ◽  
Author(s):  
Ana Bajc Česnik ◽  
Simona Darovic ◽  
Sonja Prpar Mihevc ◽  
Maja Štalekar ◽  
Mirjana Malnar ◽  
...  
Keyword(s):  
C9orf72 Expansion ◽  
Rna Foci ◽  
Nuclear Rna ◽  
Expansion Mutation

scholarly journals TCF4Triplet Repeat Expansion and Nuclear RNA Foci in Fuchs' Endothelial Corneal Dystrophy

2015 ◽  
Vol 56 (3) ◽  
pp. 2003 ◽  
Author(s):  
V. Vinod Mootha ◽  
Imran Hussain ◽  
Khrishen Cunnusamy ◽  
Eric Graham ◽  
Xin Gong ◽  
...  
Keyword(s):  
Corneal Dystrophy ◽  
Repeat Expansion ◽  
Rna Foci ◽  
Nuclear Rna

scholarly journals Targeting Toxic Nuclear RNA Foci with CRISPR-Cas13 to Treat Myotonic Dystrophy

2019 ◽  
Author(s):  
Pornthida Poosala ◽  
Sean R. Lindley ◽  
Kelly M. Anderson ◽  
Douglas M. Anderson
Keyword(s):  
Myotonic Dystrophy ◽  
Rna Binding ◽  
Fluorescent Protein ◽  
Rna Binding Proteins ◽  
Live Cells ◽  
Nuclear Retention ◽  
Rna Foci ◽  
Nuclear Rna ◽  
Nuclear Foci ◽  
Dystrophia Myotonica Protein Kinase

Human monogenetic diseases can arise from the aberrant expansion of tandem nucleotide repeat sequences, which when transcribed into RNA, can misfold and aggregate into toxic nuclear foci1. Nuclear retention of repeat-containing RNAs can disrupt their normal expression and induce widespread splicing defects by sequestering essential RNA binding proteins. Among the most prevalent of these disorders is myotonic dystrophy type 1 (DM1), a disease occurring from the expression of a noncoding CTG repeat expansion in the 3’UTR of the human dystrophia myotonica protein kinase (DMPK) gene2,3. Here we show that RNA-binding CRISPR-Cas13, with a robust non-classical nuclear localization signal, can be efficiently targeted to toxic nuclear RNA foci for either visualization or cleavage, tools we named hilightR and eraseR, respectively. HilightR combines catalytically dead Cas13b (dCas13b) with a fluorescent protein to directly visualize CUG repeat RNA foci in the nucleus of live cells, allowing for quantification of foci number and observation of foci dynamics. EraseR utilizes the intrinsic endoribonuclease activity of Cas13b, targeted to nuclear CUG repeat RNA, to disrupt nuclear foci. These studies demonstrate the potential for targeting toxic nuclear RNA foci directly with CRISPR-Cas13 for either the identification or treatment of DM1. The efficient and sequence programmable nature of CRISPR-Cas13 systems will allow for rapid targeting and manipulation of other human nuclear RNA disorders, without the associated risks of genome editing.

O.32Genome editing of expanded CTG repeats within the human DMPK gene reduces nuclear RNA foci in muscle of DM1 mice

2019 ◽  
Vol 29 ◽  
pp. S125
Author(s):  
M. Lo Scrudato ◽  
K. Poulard ◽  
C. Sourd ◽  
S. Tomé ◽  
A. Klein ◽  
...  
Keyword(s):  
Ctg Repeats ◽  
Rna Foci ◽  
Nuclear Rna ◽  
Dmpk Gene

scholarly journals Differential Toxicity of Nuclear RNA Foci versus Dipeptide Repeat Proteins in a Drosophila Model of C9ORF72 FTD/ALS

Neuron ◽  
2015 ◽  
Vol 87 (6) ◽  
pp. 1207-1214 ◽  
Author(s):  
Helene Tran ◽  
Sandra Almeida ◽  
Jill Moore ◽  
Tania F. Gendron ◽  
UmaDevi Chalasani ◽  
...  
Keyword(s):  
Dipeptide Repeat Proteins ◽  
Repeat Proteins ◽  
Rna Foci ◽  
Drosophila Model ◽  
Differential Toxicity ◽  
Nuclear Rna ◽  
Dipeptide Repeat

Low-voltage field-emission SEM of polymeric membranes for glucose biosensors

1990 ◽  
Vol 48 (3) ◽  
pp. 860-861
Author(s):  
Lorna K. Mayo ◽  
Kenneth C. Moore ◽  
Mark A. Arnold
Keyword(s):  
Glucose Sensor ◽  
Low Voltage ◽  
Glucose Sensors ◽  
Polymeric Membranes ◽  
Artificial Endocrine Pancreas ◽  
Self Monitoring ◽  
Conducting Materials ◽  
Insulin Dependent ◽  
Living Tissues ◽  
Voltage Scanning

An implantable artificial endocrine pancreas consisting of a glucose sensor and a closed-loop insulin delivery system could potentially replace the need for glucose self-monitoring and regulation among insulin dependent diabetics. Achieving such a break through largely depends on the development of an appropriate, biocompatible membrane for the sensor. Biocompatibility is crucial since changes in the glucose sensors membrane resulting from attack by orinter action with living tissues can interfere with sensor reliability and accuracy. If such interactions can be understood, however, compensations can be made for their effects. Current polymer technology offers several possible membranes that meet the unique chemical dynamics required of a glucose sensor. Two of the most promising polymer membranes are polytetrafluoroethylene (PTFE) and silicone (Si). Low-voltage scanning electron microscopy, which is an excellent technique for characterizing a variety of polymeric and non-conducting materials, 27 was applied to the examination of experimental sensor membranes.

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