scholarly journals Conformational and thermodynamic hallmarks of DNA operator site specificity in the copper sensitive operon repressor from Streptomyces lividans

2013 ◽  
Vol 42 (2) ◽  
pp. 1326-1340 ◽  
Author(s):  
B. G. Tan ◽  
E. Vijgenboom ◽  
J. A. R. Worrall
Keyword(s):  
Streptomyces Lividans ◽  
Site Specificity ◽  
Operator Site

Phenotypic dissection of bone mineral density facilitates the identification of skeletal site specificity on the genetic regulation of bone

2013 ◽  
Author(s):  
P Kemp John ◽  
Medina-Gomez Carolina ◽  
Estrada Karol ◽  
H M Heppe Denise ◽  
M Zillikens Carola ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Genetic Regulation ◽  
Site Specificity ◽  
Mineral Density ◽  
Skeletal Site

Loom weights in Archaic South Italy and Sicily: Fice case studies

2015 ◽  
Vol 8 ◽  
pp. 123-155
Author(s):  
Hedvig Landenius Enegren
Keyword(s):  
Case Studies ◽  
Indigenous Peoples ◽  
Environmental Conditions ◽  
Experimental Archaeology ◽  
Archaeological Sites ◽  
Site Specificity ◽  
Archaeological Record ◽  
South Italy ◽  
Local Traditions ◽  
Tool Technology

Textiles are perishables in the archaeological record unless specific environmental conditions are met. Fortunately, the textile tools used in their manufacture can provide a wealth of information and via experimental archaeology make visible to an extent what has been lost. The article presents and discusses the results obtained in a research project focused on textile tool technologies and identities in the context of settler and indigenous peoples, at select archaeological sites in South Italy and Sicily in the Archaic and Early Classical periods, with an emphasis on loom weights. Despite a common functional tool technology, the examined loom weights reveal an intriguing inter-site specificity, which, it is argued, is the result of hybrid expressions embedded in local traditions. Experimental archaeology testing is applied in the interpretation of the functional qualities of this common artefact.

Characterization of a carboxylesterase with hyper-thermostability and alkali-stability from Streptomyces lividans TK24

Extremophiles ◽  
2021 ◽  
Author(s):  
Xin Chang ◽  
Shuang Wu ◽  
Jie Chen ◽  
Shengqi Xiong ◽  
Peng Wang ◽  
...  
Keyword(s):  
Streptomyces Lividans ◽  
Alkali Stability

scholarly journals The Phosin PptA Plays a Negative Role in the Regulation of Antibiotic Production in Streptomyces lividans

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 325
Author(s):  
Noriyasu Shikura ◽  
Emmanuelle Darbon ◽  
Catherine Esnault ◽  
Ariane Deniset-Besseau ◽  
Delin Xu ◽  
...  
Keyword(s):  
Antibiotic Production ◽  
Streptomyces Lividans ◽  
Positive Control ◽  
Phosphate Limitation ◽  
Antibiotic Biosynthesis ◽  
Nudix Hydrolase ◽  
Energetic Stress ◽  
Accessory Factor ◽  
Negative Role ◽  
Weak Expression

In Streptomyces, antibiotic biosynthesis is triggered in phosphate limitation that is usually correlated with energetic stress. Polyphosphates constitute an important reservoir of phosphate and energy and a better understanding of their role in the regulation of antibiotic biosynthesis is of crucial importance. We previously characterized a gene, SLI_4384/ppk, encoding a polyphosphate kinase, whose disruption greatly enhanced the weak antibiotic production of Streptomyces lividans. In the condition of energetic stress, Ppk utilizes polyP as phosphate and energy donor, to generate ATP from ADP. In this paper, we established that ppk is co-transcribed with its two downstream genes, SLI_4383, encoding a phosin called PptA possessing a CHAD domain constituting a polyphosphate binding module and SLI_4382 encoding a nudix hydrolase. The expression of the ppk/pptA/SLI_4382 operon was shown to be under the positive control of the two-component system PhoR/PhoP and thus mainly expressed in condition of phosphate limitation. However, pptA and SLI_4382 can also be transcribed alone from their own promoter. The deletion of pptA resulted into earlier and stronger actinorhodin production and lower lipid content than the disruption of ppk, whereas the deletion of SLI_4382 had no obvious phenotypical consequences. The disruption of ppk was shown to have a polar effect on the expression of pptA, suggesting that the phenotype of the ppk mutant might be linked, at least in part, to the weak expression of pptA in this strain. Interestingly, the expression of phoR/phoP and that of the genes of the pho regulon involved in phosphate supply or saving were strongly up-regulated in pptA and ppk mutants, revealing that both mutants suffer from phosphate stress. Considering the presence of a polyphosphate binding module in PptA, but absence of similarities between PptA and known exo-polyphosphatases, we proposed that PptA constitutes an accessory factor for exopolyphosphatases or general phosphatases involved in the degradation of polyphosphates into phosphate.

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