scholarly journals A new strategy for gene targeting and functional proteomics using the DT40 cell line

2013 ◽  
Vol 41 (17) ◽  
pp. e167-e167 ◽  
Author(s):  
Kinga P. Orlowska ◽  
Kamila Klosowska ◽  
Roman J. Szczesny ◽  
Dominik Cysewski ◽  
Pawel S. Krawczyk ◽  
...  
Keyword(s):  
Cell Line ◽  
Gene Targeting ◽  
Functional Proteomics ◽  
Dt40 Cell ◽  
New Strategy

scholarly journals A homozygous Keap1-knockout human embryonic stem cell line generated using CRISPR/Cas9 mediates gene targeting

2017 ◽  
Vol 19 ◽  
pp. 52-54 ◽  
Author(s):  
So-Jung Kim ◽  
Omer Habib ◽  
Jin-Soo Kim ◽  
Hyo-Won Han ◽  
Soo Kyung Koo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Line ◽  
Gene Targeting ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Stem Cell Line

scholarly journals Validation of the Reference Genes for the Gene Expression Studies in Chicken DT40 Cell Line

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 372
Author(s):  
Aleksandra Dunislawska ◽  
Anna Slawinska ◽  
Maria Siwek
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Reference Gene ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Relative Gene Expression ◽  
Dt40 Cell ◽  
Relative Gene ◽  
Chicken Dt40 Cell ◽  
Selection Of

The selection of a suitable reference gene assures a reliable gene expression analysis when using the qPCR method. Normalization of the reaction is based on the basic metabolism genes. These genes show a constant, unregulated expression in all cells and function throughout their lifetime. In the current study, seven reference gene candidates were screened using RT-qPCR, to determine the best-matched pair of reference genes in the chicken DT40 cell line. The DT40 was derived from bursal lymphoma cells that were subjected to RAV-1 bird retroviral infection. It is a simplified in vitro model that allows tracking the direct interaction of stimulants on the lymphoid population and profiling of the hepatocellular B cell transcriptome. The reference gene analysis was carried out using statistical tools integrating four independent methods—geNorm, Best Keeper, NormFinder, delta Ct and RefFinder. Based on the selected reference genes, the relative gene expression analysis was done using the ddCt method. Complete relative gene expression study on a panel of the target genes revealed that proper selection of reference genes depending on the tissue eliminate decreases in data quality. The SDHA and RPL4 genes constitute stable internal controls as reference genes when analyzing gene expression in the DT40 cell line.

scholarly journals Combinatorial gene targeting hTERT and BI-1 in CNE-2 nasopharyngeal carcinoma cell line

2012 ◽  
Vol 1 (2) ◽  
pp. 285-293 ◽  
Author(s):  
YAN LIANG ◽  
XIANGYONG LI ◽  
RONGWEN LIN ◽  
XIN ZHANG ◽  
HUIMIN WANG ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Line ◽  
Gene Targeting ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Nasopharyngeal Carcinoma Cell

scholarly journals Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction

2013 ◽  
Vol 77 (4) ◽  
pp. 228-233 ◽  
Author(s):  
Eiko N. Minakawa ◽  
Hodaka Yamakado ◽  
Atsushi Tanaka ◽  
Kengo Uemura ◽  
Shunichi Takeda ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Mitochondrial Dysfunction ◽  
Cell Line ◽  
Dt40 Cell ◽  
Familial Parkinson’S Disease ◽  
Chicken Dt40 Cell
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