scholarly journals Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

2013 ◽  
Vol 41 (14) ◽  
pp. 7176-7183 ◽  
Author(s):  
Kei Fujiwara ◽  
Tsutomu Katayama ◽  
Shin-ichiro M. Nomura
Keyword(s):  
Protein Expression ◽  
Expression System ◽  
Chromosome Replication ◽  
Bacterial Chromosome ◽  
Replication Proteins ◽  
Protein Expression System ◽  
Reconstituted Protein

Optimization of a Fusion Protein Expression System using Human Cell Lines to Create a Practical Immunoassay Reagent

2015 ◽  
Vol 41 (1) ◽  
pp. 38-42 ◽  
Author(s):  
Kounosuke Hayashi ◽  
Yusuke Tomozoe ◽  
Kenji Nagai ◽  
Kyoichi Matsuba ◽  
Masayuki Mitsumori ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Protein Expression ◽  
Cell Lines ◽  
Human Cell ◽  
Expression System ◽  
Human Cell Lines ◽  
Fusion Protein Expression ◽  
Protein Expression System

scholarly journals Expression of Recombinant Fusion Protein of Newcastle Disease Virus from Escherichia coli Plasmid Clone C-2a by In-vitro Cell-free Protein Expression System

2020 ◽  
Vol 20 ◽  
pp. 04004
Author(s):  
Ahmad Pandu Satria Wiratama ◽  
Aris Haryanto
Keyword(s):  
Protein Expression ◽  
Newcastle Disease ◽  
Recombinant Plasmid ◽  
Expression System ◽  
Free Protein ◽  
F Protein ◽  
E Coli ◽  
Protein Expression System ◽  
Sds Page

Newcastle Disease Virus (NDV) is an infectious disease that infect many kinds of wild and domesticated birds. Infection of NDV become a massive problem for poultry industry around the world especially in Indonesia. Vaccination is an effort to prevent the infection of NDV in poultry. NDV vaccine that used in Indonesia is a conventional life vaccine from LaSota and B1 strains. These type of vaccine is 21%-23% genetically distinct with the virus that spread in the environment. The antibody protection provided by the vaccine is not effective. Therefore, vaccination with new local NDV strain is needed to prevent the NDV infection in Indonesia. The previously study research reported that the local isolate of NDV from Kulon Progo, Indonesia has been isolated. Fusion (F) protein encoding gene that has been inserted into pBT7-N-His expression p lasmid which isolated from clone C-2a of E. coli, then it was expressed by the Cell-free protein expression system. The aim of this study was to confirm whether clone C-2a of E.coli carrying a recombinant plasmid pBT7-N-His-Fusion NDV and to express a recombinant F protein of NDV in-vitro from expression plasmid by cell-free protein expression system. This work started by detection of recombinant plasmid pBT7-N-His-Fusion NDV by DNA plasmid extraction followed by agarose gel electrophoresis. The recombinant F protein was in-vitro expressed by cell-free protein expression kit. The expressed F protein of NDV then was visualized by SDS-PAGE and Westernblott to analyse the expression of NDV recombinant F protein. It confirmed that clone C-2a of E. coli contained plasmid pBT7-N-His (4.001 bp) inserted by recombinant F protein of NDV gene (642 bp). The visualisation of expressed recombinant F protein by SDS-PAGE and Westernblott showed the NDV recombinant F protein was a specific protein fragment with molecular weight of 25,6 kDa..

scholarly journals The establishment of new protein expression system using N starvation inducible promoters in Chlorella

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun-Hye Shin ◽  
Juyoung Choi ◽  
Jeongmin Jeon ◽  
Manu Kumar ◽  
Juhyeon Lee ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression System ◽  
Inducible Promoters ◽  
Protein Expression System ◽  
N Starvation ◽  
New Protein

A Multifunctional Prokaryotic Protein Expression System: Overproduction, Affinity Purification, and Selective Detection

1993 ◽  
Vol 12 (5) ◽  
pp. 441-453 ◽  
Author(s):  
DAVID J. KROLL ◽  
HANY ABDEL-MALEK ABDEL-HAFIZ ◽  
TERE MARCELL ◽  
SEAN SIMPSON ◽  
CHANG-YOU CHEN ◽  
...  
Keyword(s):  
Protein Expression ◽  
Affinity Purification ◽  
Expression System ◽  
Selective Detection ◽  
Protein Expression System ◽  
Prokaryotic Protein

scholarly journals A self-inducible heterologous protein expression system in Escherichia coli

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
L. Briand ◽  
G. Marcion ◽  
A. Kriznik ◽  
J. M. Heydel ◽  
Y. Artur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
Heterologous Protein ◽  
Expression System ◽  
Heterologous Protein Expression ◽  
Protein Expression System

A T7 promoter-specific, inducible protein expression system for

1996 ◽  
Vol 250 (2) ◽  
pp. 230 ◽  
Author(s):  
Birgit Conrad ◽  
R. S. Savchenko ◽  
Roland Breves ◽  
J. Hofemeister
Keyword(s):  
Protein Expression ◽  
Expression System ◽  
T7 Promoter ◽  
Protein Expression System ◽  
Inducible Protein
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