scholarly journals DNA G-quadruplex formation in response to remote downstream transcription activity: long-range sensing and signal transducing in DNA double helix

2013 ◽  
Vol 41 (14) ◽  
pp. 7144-7152 ◽  
Author(s):  
C. Zhang ◽  
H.-h. Liu ◽  
K.-w. Zheng ◽  
Y.-h. Hao ◽  
Z. Tan
Keyword(s):  
Long Range ◽  
Double Helix ◽  
Range Sensing ◽  
Transcription Activity ◽  
G Quadruplex ◽  
Quadruplex Formation ◽  
Dna Double Helix

scholarly journals Novel Indolocarbazole Derivative 12-(α-L-arabinopyranosyl)indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7-dione Is a Preferred c-MycGuanine Quadruplex Ligand

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Dmitry N. Kaluzhny ◽  
Anna K. Shchyolkina ◽  
Nikolay S. Ilyinsky ◽  
Olga F. Borisova ◽  
Alexander A. Shtil
Keyword(s):  
Double Helix ◽  
Human Tumor ◽  
State Level ◽  
Transplanted Tumors ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
G Quadruplex ◽  
Preferential Binding ◽  
Quadruplex Formation ◽  
Dna Double Helix

The indolocarbazole derivative 12-(α-L-arabinopyranosyl)indolo[2,3-a]pyrrolo[3,4-c]carbazole-5,7-dione (AIC) has demonstrated a high potency (at nanomolar to submicromolar concentrations) towards the NCI panel of human tumor cell lines and transplanted tumors. Intercalation into the DNA double helix has been identified as an important prerequisite for AIC cytotoxicity. In this study, we provide evidence for preferential binding to the G-quadruplex derived from the c-Myconcogene promoter (Pu18 d(AG3TG4)2; G-c-Myc). The association constant for AIC:G-c-Myccomplex was ~100 times and 10 times greater than the respective values for the complexes AIC:c-Mycduplex and AIC:telomeric d(TTAGGG)4G-quadruplex. The concentrations at which AIC formed complexes with G-c-Mycwere close to those that attenuated the steady-state level of the c-MycmRNA in the human HCT116 colon carcinoma cell line. We suggest that preferential binding of AIC to G-c-Mycrather than to the c-Mycduplex might favor the quadruplex formation in the cells, thereby contributing to downregulation of the c-Mycexpression by AIC.

DNA double-helix-mediated long-range electron transfer

1996 ◽  
Vol 60 (8) ◽  
pp. 1789-1795 ◽  
Author(s):  
Satyam Priyadarshy ◽  
David N. Beratan ◽  
Steven M. Risser
Keyword(s):  
Electron Transfer ◽  
Long Range ◽  
Double Helix ◽  
Dna Double Helix

scholarly journals NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes

2019 ◽  
Vol 47 (15) ◽  
pp. 8272-8281 ◽  
Author(s):  
Fernaldo Richtia Winnerdy ◽  
Blaž Bakalar ◽  
Arijit Maity ◽  
J Jeya Vandana ◽  
Yves Mechulam ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Double Helix ◽  
Quadruple Helix ◽  
Single Nucleotide ◽  
Quadruplex Dna ◽  
Dna Structures ◽  
X Ray ◽  
Left Handed ◽  
G Quadruplex ◽  
Quadruplex Formation

AbstractAnalogous to the B- and Z-DNA structures in double-helix DNA, there exist both right- and left-handed quadruple-helix (G-quadruplex) DNA. Numerous conformations of right-handed and a few left-handed G-quadruplexes were previously observed, yet they were always identified separately. Here, we present the NMR solution and X-ray crystal structures of a right- and left-handed hybrid G-quadruplex. The structure reveals a stacking interaction between two G-quadruplex blocks with different helical orientations and displays features of both right- and left-handed G-quadruplexes. An analysis of loop mutations suggests that single-nucleotide loops are preferred or even required for the left-handed G-quadruplex formation. The discovery of a right- and left-handed hybrid G-quadruplex further expands the polymorphism of G-quadruplexes and is potentially useful in designing a left-to-right junction in G-quadruplex engineering.

Protamine-DNA interaction

1978 ◽  
Vol 36 (2) ◽  
pp. 200-201
Author(s):  
D.P. Bazett-Jones ◽  
F.P. Ottensmeyer
Keyword(s):  
Small Volume ◽  
Double Helix ◽  
Dna Interaction ◽  
Dark Field ◽  
Sperm Head ◽  
Nuclear Proteins ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dna Double Helix ◽  
Positively Charged

Dark field electron microscopy has been used for the study of the structure of individual macromolecules with a resolution to at least the 5Å level. The use of this technique has been extended to the investigation of structure of interacting molecules, particularly the interaction between DNA and fish protamine, a class of basic nuclear proteins of molecular weight 4,000 daltons.Protamine, which is synthesized during spermatogenesis, binds to chromatin, displaces the somatic histones and wraps up the DNA to fit into the small volume of the sperm head. It has been proposed that protamine, existing as an extended polypeptide, winds around the minor groove of the DNA double helix, with protamine's positively-charged arginines lining up with the negatively-charged phosphates of DNA. However, viewing protamine as an extended protein is inconsistent with the results obtained in our laboratory.

scholarly journals DNA polymerase δ stalls on telomeric lagging strand templates independently from G-quadruplex formation

2013 ◽  
Vol 41 (22) ◽  
pp. 10323-10333 ◽  
Author(s):  
Justin D. Lormand ◽  
Noah Buncher ◽  
Connor T. Murphy ◽  
Parminder Kaur ◽  
Marietta Y. Lee ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase Δ ◽  
G Quadruplex ◽  
Quadruplex Formation ◽  
Lagging Strand

scholarly journals TGF‐β‐induced fibrotic stress increases G‐quadruplex formation in human fibroblasts

FEBS Letters ◽  
2019 ◽  
Vol 593 (22) ◽  
pp. 3149-3161 ◽  
Author(s):  
Priyanka Toshniwal ◽  
Michelle Nguyen ◽  
Aurore Guédin ◽  
Helena Viola ◽  
Diwei Ho ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
G Quadruplex ◽  
Quadruplex Formation
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