scholarly journals Tye7 regulates yeast Ty1 retrotransposon sense and antisense transcription in response to adenylic nucleotides stress

2012 ◽  
Vol 40 (12) ◽  
pp. 5271-5282 ◽  
Author(s):  
Géraldine Servant ◽  
Benoit Pinson ◽  
Aurélie Tchalikian-Cosson ◽  
Fanny Coulpier ◽  
Sophie Lemoine ◽  
...  
Keyword(s):  
Antisense Transcription ◽  
Ty1 Retrotransposon

Primate-Specific Endogenous Cis-Antisense Transcription in the Human 5q31 Protocadherin Gene Cluster

2005 ◽  
Vol 62 (1) ◽  
pp. 73-88 ◽  
Author(s):  
Leonard Lipovich ◽  
Ravi Raj Vanisri ◽  
Say Li Kong ◽  
Chin-Yo Lin ◽  
Edison T. Liu
Keyword(s):  
Gene Cluster ◽  
Antisense Transcription

scholarly journals Episomal Expression of Specific Sense and Antisense mRNAs in Leishmania amazonensis: Modulation of gp63 Level in Promastigotes and Their Infection of Macrophages In Vitro

2000 ◽  
Vol 68 (1) ◽  
pp. 80-86 ◽  
Author(s):  
De-Qiao Chen ◽  
Bala Krishna Kolli ◽  
Nagendra Yadava ◽  
Hong Gang Lu ◽  
Alice Gilman-Sachs ◽  
...  
Keyword(s):  
Kinetic Studies ◽  
Single Copy ◽  
Antisense Transcription ◽  
Leishmania Amazonensis ◽  
Surface Glycoprotein ◽  
Major Surface Glycoprotein ◽  
Wild Type Clone ◽  
Major Surface ◽  
Specific Sense

ABSTRACT The major surface glycoprotein (gp63) of Leishmania amazonensis is a metalloprotease implicated in the infection of mammalian macrophages. The expression of gp63 and its participation in this infection were further examined by modulating the level of this molecule in a virulent gp63-abundant wild-type clone. Promastigotes were transfected with gp63 genes cloned into aLeishmania-specific vector in two different orientations, leading to the expression of gp63 sense and antisense RNAs. With increasing selective pressure, cell surface gp63 was increasingly augmented in the transfectants with sense transcripts and suppressed to a very low level in those with antisense transcripts. Thus, the expression of gp63 from chromosomal, repetitive genes is not stringently regulated at the protein level and can be substantially reduced by episomal antisense transcription of a single copy. The transfectants differed significantly only in the level of gp63, thereby allowing specific evaluation of this molecule in leishmanial infection of macrophages in vitro. Kinetic studies of infection in vitro indicate that gp63 plays a role not only in the binding of this parasite to these macrophages but also in its intramacrophage survival and replication.

An antisense promoter of the murine c-myc gene is localized within intron 2

1992 ◽  
Vol 12 (3) ◽  
pp. 1324-1329
Author(s):  
D B Spicer ◽  
G E Sonenshein
Keyword(s):  
Antisense Transcription ◽  
In Vitro Transcription ◽  
Region Gene ◽  
S1 Nuclease ◽  
Gene Translocation ◽  
Antisense Orientation ◽  
Nuclear Extracts ◽  
Myc Gene ◽  
Intron 2

Previously we have demonstrated the existence of stable transcripts from the noncoding strand of a rearranged c-myc gene in murine plasmacytomas in which the oncogene has translocated to an immunoglobulin constant-region gene element (M. Dean, R. B. Kent, and G. E. Sonenshein, Nature [London] 305:443-446, 1983). The resulting RNAs are chimeric, containing c-myc antisense and immunoglobulin sense sequences. A normal unrearranged murine c-myc gene is transcribed in the antisense orientation throughout much of the gene; however, stable transcripts have not been detected. In this study, using Northern (RNA) blot, S1 nuclease, and primer extension analyses, we have mapped the 5' end of the stable chimeric transcripts to a site 175 bp from the start of exon 3, within intron 2 of the c-myc gene. In vitro transcription assays with constructs containing this site and 400 bp upstream, in the antisense orientation, and nuclear extracts from plasmacytoma cells, as well as a number of cell lines with normal unrearranged c-myc genes, indicated that this promoter was functional. This finding was confirmed in transient transfection assays using the antisense promoter linked to the chloramphenicol acetyltransferase reporter gene. These results suggest that a normal promoter of antisense transcription is used following c-myc gene translocation.

scholarly journals Antisense Transcription in Gammaretroviruses as a Mechanism of Insertional Activation of Host Genes

2010 ◽  
Vol 84 (8) ◽  
pp. 3780-3788 ◽  
Author(s):  
Mads Heilskov Rasmussen ◽  
Borja Ballarín-González ◽  
Jinghua Liu ◽  
Louise Berkhoudt Lassen ◽  
Annette Füchtbauer ◽  
...  
Keyword(s):  
B Cell ◽  
Leukemia Virus ◽  
Negative Polarity ◽  
Antisense Transcription ◽  
B Cell Lymphomas ◽  
Eukaryotic Transcription ◽  
R Region ◽  
Rapid Amplification ◽  
Host Genes

ABSTRACT Transcription of retroviruses is initiated at the U3-R region boundary in the integrated provirus and continues unidirectionally to produce genomic and mRNA products of positive polarity. Several studies have recently demonstrated the existence of naturally occurring protein-encoding transcripts of negative polarity in complex retroviruses. We report here on the identification of transcripts of negative polarity in simple murine leukemia virus (MLV). In T-cell and B-cell lymphomas induced by SL3-3 and Akv MLV, antisense transcripts initiated in the U3 region of the proviral 5′ long terminal repeat (LTR) and continued into the cellular proto-oncogenes Jdp2 and Bach2 to create chimeric transcripts consisting of viral and host sequence. The phenomenon was validated in vivo using a knock-in mouse model homozygous for a single LTR at a position known to activate Nras in B-cell lymphomas. A 5′ rapid amplification of cDNA ends (RACE) analysis indicated a broad spectrum of initiation sites within the U3 region of the 5′ LTR. Our data show for the first time transcriptional activity of negative polarity initiating in the U3 region of simple retroviruses and suggest a novel mechanism of insertional activation of host genes. Elucidation of the nature and potential regulatory role of 5′ LTR antisense transcription will be relevant to the design of therapeutic vectors and may contribute to the increasing recognition of pervasive eukaryotic transcription.

scholarly journals A pre-initiation complex at the 3′-end of genes drives antisense transcription independent of divergent sense transcription

2011 ◽  
Vol 40 (6) ◽  
pp. 2432-2444 ◽  
Author(s):  
Struan C. Murray ◽  
Ana Serra Barros ◽  
David A. Brown ◽  
Peter Dudek ◽  
Jonathan Ayling ◽  
...  
Keyword(s):  
Antisense Transcription ◽  
Initiation Complex

scholarly journals BisMapR: a strand-specific, nuclease-based method for genome-wide R-loop detection

2021 ◽  
Author(s):  
Phillip Wulfridge ◽  
Kavitha Sarma
Keyword(s):  
Genome Stability ◽  
Antisense Transcription ◽  
Gene Promoters ◽  
Normal Cells ◽  
Disease States ◽  
Detection Strategy ◽  
Genome Wide ◽  
Loop Detection ◽  
Nucleic Acid Structures ◽  
Nuclear Processes

AbstractR-loops are three stranded nucleic acid structures with essential roles in many nuclear processes. However, their unchecked accumulation as seen in some neurodevelopmental diseases and cancers and is associated with compromised genome stability. Genome-wide profiling of R-loops in normal cells and their comparison in disease states can help identify precise locations of pathogenic R-loops and advance efforts to attenuate deviant R-loops while preserving biologically important ones. Toward this, we have developed an antibody-independent R-loop detection strategy, BisMapR, that combines nuclease-based R-loop isolation with non-denaturing bisulfite chemistry to produce high-resolution, genome-wide R-loop profiles that retain strand information. Furthermore, BisMapR achieves greater resolution and is faster than existing strand-specific R-loop profiling strategies. We applied BisMapR to reveal discrete R-loop behavior at gene promoters and enhancers. We show that gene promoters exhibiting antisense transcription form R-loops in both directions. and uncover a subset of active enhancers that, despite being bidirectionally transcribed, form R-loops exclusively on one strand. Thus, BisMapR reveals a previously unnoticed feature of active enhancers and provides a tool to systematically examine their mechanisms in gene expression.

scholarly journals Gene Isoform Specificity through Enhancer-Associated Antisense Transcription

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43511 ◽  
Author(s):  
Courtney S. Onodera ◽  
Jason G. Underwood ◽  
Sol Katzman ◽  
Frank Jacobs ◽  
David Greenberg ◽  
...  
Keyword(s):  
Antisense Transcription ◽  
Isoform Specificity ◽  
Gene Isoform

scholarly journals Antisense transcription at the TRPM2 locus as a novel prognostic marker and therapeutic target in prostate cancer

Oncogene ◽  
2014 ◽  
Vol 34 (16) ◽  
pp. 2094-2102 ◽  
Author(s):  
U Orfanelli ◽  
E Jachetti ◽  
F Chiacchiera ◽  
M Grioni ◽  
P Brambilla ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prognostic Marker ◽  
Therapeutic Target ◽  
Antisense Transcription
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