scholarly journals Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes

2011 ◽  
Vol 40 (1) ◽  
pp. 148-158 ◽  
Author(s):  
Michal Mokry ◽  
Pantelis Hatzis ◽  
Jurian Schuijers ◽  
Nico Lansu ◽  
Frans-Paul Ruzius ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Steady State ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Functional Gene ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Transcription Factor Occupancy ◽  
Rna Levels

scholarly journals Genome‐wide analysis of FOXO3 mediated transcription regulation through RNA polymerase II profiling

2013 ◽  
Vol 9 (1) ◽  
pp. 638 ◽  
Author(s):  
Astrid Eijkelenboom ◽  
Michal Mokry ◽  
Elzo de Wit ◽  
Lydia M Smits ◽  
Paulien E Polderman ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcription Regulation ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II

Genomics Data ◽  
2016 ◽  
Vol 7 ◽  
pp. 250-255 ◽  
Author(s):  
Ryan P. McNamara ◽  
Carlos Guzman ◽  
Jonathan E. Reeder ◽  
Iván D'Orso
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Genome-wide analysis in Plasmodium falciparum reveals early and late phases of RNA polymerase II occupancy during the infectious cycle

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 959 ◽  
Author(s):  
Ragini Rai ◽  
Lei Zhu ◽  
Haifen Chen ◽  
Archana Gupta ◽  
Siu Sze ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Infectious Cycle

scholarly journals Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes

2017 ◽  
Vol 66 (1) ◽  
pp. 38-49.e6 ◽  
Author(s):  
Carlo Baejen ◽  
Jessica Andreani ◽  
Phillipp Torkler ◽  
Sofia Battaglia ◽  
Bjoern Schwalb ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Protein Coding ◽  
Genome Wide Analysis ◽  
Protein Coding Genes ◽  
Genome Wide

scholarly journals Underproduction of the Largest Subunit of RNA Polymerase II Causes Temperature Sensitivity, Slow Growth, and Inositol Auxotrophy in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 737-747 ◽  
Author(s):  
Jacques Archambault ◽  
David B Jansma ◽  
James D Friesen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Steady State ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Reporter Gene ◽  
Growth Medium ◽  
Temperature Sensitivity ◽  
Slow Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Genes Encoding

Abstract In the yeast Saccharomyces cerevisiae, mutations in genes encoding subunits of RNA polymerase II (RNAPII) often give rise to a set of pleiotropic phenotypes that includes temperature sensitivity, slow growth and inositol auxotrophy. In this study, we show that these phenotypes can be brought about by a reduction in the intracellular concentration of RNAPII. Underproduction of RNAPII was achieved by expressing the gene (RPO21), encoding the largest subunit of the enzyme, from the LEU2 promoter or a weaker derivative of it, two promoters that can be repressed by the addition of leucine to the growth medium. We found that cells that underproduced RPO21 were unable to derepress fully the expression of a reporter gene under the control of the INO1 UAS. Our results indicate that temperature sensitivity, slow growth and inositol auxotrophy is a set of phenotypes that can be caused by lowering the steady-state amount of RNAPII; these results also lead to the prediction that some of the previously identified RNAPII mutations that confer this same set of phenotypes affect the assembly/stability of the enzyme. We propose a model to explain the hypersensitivity of INO1 transcription to mutations that affect components of the RNAPII transcriptional machinery.

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