scholarly journals Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex

2009 ◽  
Vol 37 (17) ◽  
pp. 5803-5809 ◽  
Author(s):  
Joanna Andrecka ◽  
Barbara Treutlein ◽  
Maria Angeles Izquierdo Arcusa ◽  
Adam Muschielok ◽  
Robert Lewis ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Positioning System ◽  
Elongation Complex

Allosteric transcription stimulation by RNA polymerase II super elongation complex

2021 ◽  
Author(s):  
Ying Chen ◽  
Seychelle M. Vos ◽  
Christian Dienemann ◽  
Momchil Ninov ◽  
Henning Urlaub ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Elongation Complex ◽  
Super Elongation Complex

scholarly journals Contacts between mammalian RNA polymerase II and the template DNA in a ternary elongation complex

1993 ◽  
Vol 21 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Gretchen A. Rice ◽  
Michael J. Chamberlin ◽  
Caroline M. Kane
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Elongation Complex ◽  
Template Dna

Structure of the complete elongation complex of RNA polymerase II with basal factors

Science ◽  
2017 ◽  
Vol 357 (6354) ◽  
pp. 921-924 ◽  
Author(s):  
Haruhiko Ehara ◽  
Takeshi Yokoyama ◽  
Hideki Shigematsu ◽  
Shigeyuki Yokoyama ◽  
Mikako Shirouzu ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Elongation Complex

scholarly journals Role of the mammalian transcription factors IIF, IIS, and IIX during elongation by RNA polymerase II.

1991 ◽  
Vol 11 (3) ◽  
pp. 1195-1206 ◽  
Author(s):  
E Bengal ◽  
O Flores ◽  
A Krauskopf ◽  
D Reinberg ◽  
Y Aloni
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Gel Filtration ◽  
Late Promoter ◽  
Elongation Complex ◽  
Cell Extracts ◽  
Adenovirus Major Late Promoter ◽  
Major Late Promoter ◽  
Transcription Complexes

We have used a recently developed system that allows the isolation of complexes competent for RNA polymerase II elongation (E. Bengal, A. Goldring, and Y. Aloni, J. Biol. Chem. 264:18926-18932, 1989). Pulse-labeled transcription complexes were formed at the adenovirus major late promoter with use of HeLa cell extracts. Elongation-competent complexes were purified from most of the proteins present in the extract, as well as from loosely bound elongation factors, by high-salt gel filtration chromatography. We found that under these conditions the nascent RNA was displaced from the DNA during elongation. These column-purified complexes were used to analyze the activities of different transcription factors during elongation by RNA polymerase II. We found that transcription factor IIS (TFIIS), TFIIF, and TFIIX affected the efficiency of elongation through the adenovirus major late promoter attenuation site and a synthetic attenuation site composed of eight T residues. These factors have distinct activities that depend on whether they are added before RNA polymerase has reached the attenuation site or at the time when the polymerase is pausing at the attenuation site. TFIIS was found to have antiattenuation activity, while TFIIF and TFIIX stimulated the rate of elongation. In comparison with TFIIF, TFIIS is loosely bound to the elongation complex. We also found that the activities of the factors are dependent on the nature of the attenuator. These results indicate that at least three factors play a major role during elongation by RNA polymerase II.

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