scholarly journals A genetic screen for components of the mammalian RNA interference pathway in Bloom-deficient mouse embryonic stem cells

2008 ◽  
Vol 37 (4) ◽  
pp. e34-e34 ◽  
Author(s):  
M. I. Trombly ◽  
H. Su ◽  
X. Wang
Keyword(s):  
Stem Cells ◽  
Rna Interference ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Genetic Screen ◽  
Deficient Mouse

Increased apoptosis in differentiating p27-deficient mouse embryonic stem cells

2004 ◽  
Vol 61 (11) ◽  
pp. 1384-1400 ◽  
Author(s):  
V. Bryja ◽  
J. Pachern�k ◽  
K. Soucek ◽  
V. Horvath ◽  
P. Dvor�k ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Deficient Mouse

scholarly journals Correction: Functional Analysis of Tcl1 Using Tcl1-Deficient Mouse Embryonic Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
Author(s):  
Tatsushi Miyazaki ◽  
Satsuki Miyazaki ◽  
Masafumi Ashida ◽  
Tomofumi Tanaka ◽  
Fumi Tashiro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Functional Analysis ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Deficient Mouse

scholarly journals Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells

2007 ◽  
Vol 12 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Kohta Ikegami ◽  
Misa Iwatani ◽  
Masako Suzuki ◽  
Makoto Tachibana ◽  
Yoichi Shinkai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Deficient Mouse ◽  
Dna Hypomethylation ◽  
Genome Wide

scholarly journals Normal DNA Methylation Dynamics in DICER1-Deficient Mouse Embryonic Stem Cells

PLoS Genetics ◽  
2012 ◽  
Vol 8 (9) ◽  
pp. e1002919 ◽  
Author(s):  
Jonathan Ip ◽  
Paul Canham ◽  
K. H. Andy Choo ◽  
Yoshimi Inaba ◽  
Shelley A. Jacobs ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Deficient Mouse

scholarly journals RTEL1 contributes to DNA replication and repair and telomere maintenance

2012 ◽  
Vol 23 (14) ◽  
pp. 2782-2792 ◽  
Author(s):  
Evert-Jan Uringa ◽  
Kathleen Lisaingo ◽  
Hilda A. Pickett ◽  
Julie Brind'Amour ◽  
Jan-Hendrik Rohde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dna Repair ◽  
Dna Replication ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Gene Replacement ◽  
Mouse Embryonic Stem Cells ◽  
Telomere Maintenance ◽  
Dominant Factor ◽  
Deficient Mouse

Telomere maintenance and DNA repair are important processes that protect the genome against instability. mRtel1, an essential helicase, is a dominant factor setting telomere length in mice. In addition, mRtel1 is involved in DNA double-strand break repair. The role of mRtel1 in telomere maintenance and genome stability is poorly understood. Therefore we used mRtel1-deficient mouse embryonic stem cells to examine the function of mRtel1 in replication, DNA repair, recombination, and telomere maintenance. mRtel1-deficient mouse embryonic stem cells showed sensitivity to a range of DNA-damaging agents, highlighting its role in replication and genome maintenance. Deletion of mRtel1 increased the frequency of sister chromatid exchange events and suppressed gene replacement, demonstrating the involvement of the protein in homologous recombination. mRtel1 localized transiently at telomeres and is needed for efficient telomere replication. Of interest, in the absence of mRtel1, telomeres in embryonic stem cells appeared relatively stable in length, suggesting that mRtel1 is required to allow extension by telomerase. We propose that mRtel1 is a key protein for DNA replication, recombination, and repair and efficient elongation of telomeres by telomerase.

Sign in / Sign up

Export Citation Format

Share Document