scholarly journals Regulating amyloid precursor protein synthesis through an internal ribosomal entry site

2008 ◽  
Vol 36 (21) ◽  
pp. 6835-6847 ◽  
Author(s):  
Monique E. Beaudoin ◽  
Vincent-Joseph Poirel ◽  
Leslie A. Krushel
Keyword(s):  
Protein Synthesis ◽  
Amyloid Precursor Protein ◽  
Internal Ribosomal Entry Site ◽  
Precursor Protein ◽  
Entry Site ◽  
Ribosomal Entry ◽  
Amyloid Precursor Protein Synthesis

scholarly journals A High-Throughput Drug Screen Targeted to the 5'Untranslated Region of Alzheimer Amyloid Precursor Protein mRNA

2006 ◽  
Vol 11 (5) ◽  
pp. 469-480 ◽  
Author(s):  
Sanghamitra Bandyopadhyay ◽  
Jake Ni ◽  
Amy Ruggiero ◽  
Karen Walshe ◽  
Mark S. Rogers ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
High Throughput ◽  
Translational Control ◽  
Untranslated Region ◽  
Fluorescent Protein ◽  
Internal Ribosomal Entry Site ◽  
Precursor Protein ◽  
Luciferase Reporter ◽  
Entry Site ◽  
Novel Approach

The authors employed a novel approach to identify therapeutics effective in Alzheimer disease (AD). The 5'untranslated region (5'UTR) of the mRNA of AD amyloid precursor protein (APP) is a significant regulator of the levels of the APP holoprotein and amyloid beta (Aβ) peptide in the central nervous system. The authors generated stable neuroblastoma SH-SY5Y transfectants that express luciferase under the translational control of the 146-nucleotide APP mRNA 5'UTR and green fluorescent protein (GFP) driven by a viral internal ribosomal entry site. Using a high-throughput screen (HTS), they screened for the effect of 110,000 compounds obtained from the library of the Laboratory for Drug Discovery on Neurodegeneration (LDDN) on the APP mRNA 5'UTR-controlled translation of the luciferase reporter. This screening yielded several nontoxic specific inhibitors of APP mRNA 5'UTR-driven luciferase that had no effect on the GFP expression in the stable SH-SY5Y transfectants. Moreover, these compounds either did not inhibit or inhibited to a much lower extent the expression of the luciferase reporter regulated by a prion protein (PrP) mRNA 5'UTR, used as an alternative mRNA structure to counterscreen APP mRNA 5'UTR in stably transfected SH-SY5Y cell lines. The hits obtained from this robust, specific, and highly quantitative HTS will be characterized to identify agents that may be developed into useful future therapeutic agents to limit APP translation and Aβ production for AD.

scholarly journals CRISPR-Cas13a mediated targeting of hepatitis C virus internal-ribosomal entry site (IRES) as an effective antiviral strategy

2021 ◽  
Vol 136 ◽  
pp. 111239
Author(s):  
Muhammad Usman Ashraf ◽  
Hafiz Muhammad Salman ◽  
Muhammad Farhan Khalid ◽  
Muhammad Haider Farooq Khan ◽  
Saima Anwar ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Internal Ribosomal Entry Site ◽  
Entry Site ◽  
Ribosomal Entry

scholarly journals Riboproteomics of the Hepatitis C Virus Internal Ribosomal Entry Site

2004 ◽  
Vol 3 (5) ◽  
pp. 949-957 ◽  
Author(s):  
Henry Lu ◽  
Weiqun Li ◽  
William Stafford Noble ◽  
Donald Payan ◽  
D. C. Anderson
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Internal Ribosomal Entry Site ◽  
Entry Site ◽  
Ribosomal Entry

scholarly journals An internal ribosomal entry site mediates redox-sensitive translation of Nrf2

2009 ◽  
Vol 38 (3) ◽  
pp. 778-788 ◽  
Author(s):  
Wenge Li ◽  
Nehal Thakor ◽  
Eugenia Y. Xu ◽  
Ying Huang ◽  
Chi Chen ◽  
...  
Keyword(s):  
Internal Ribosomal Entry Site ◽  
Entry Site ◽  
Ribosomal Entry
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