scholarly journals Trace amounts of 8-oxo-dGTP in mitochondrial dNTP pools reduce DNA polymerase   replication fidelity

2008 ◽  
Vol 36 (7) ◽  
pp. 2174-2181 ◽  
Author(s):  
Z. F. Pursell ◽  
J. T. McDonald ◽  
C. K. Mathews ◽  
T. A. Kunkel
Keyword(s):  
Dna Polymerase ◽  
Replication Fidelity

scholarly journals MutS regulates access of the error-prone DNA polymerase Pol IV to replication sites: a novel mechanism for maintaining replication fidelity

2016 ◽  
Vol 44 (16) ◽  
pp. 7700-7713 ◽  
Author(s):  
Lucía M. Margara ◽  
Marisa M. Fernández ◽  
Emilio L. Malchiodi ◽  
Carlos E. Argaraña ◽  
Mariela R. Monti
Keyword(s):  
Dna Polymerase ◽  
Replication Fidelity ◽  
Pol Iv ◽  
Replication Sites

scholarly journals The Roles of Tyr391 and Tyr619 in RB69 DNA Polymerase Replication Fidelity

2007 ◽  
Vol 368 (1) ◽  
pp. 18-29 ◽  
Author(s):  
Agata Jacewicz ◽  
Karolina Makiela ◽  
Andrzej Kierzek ◽  
John W. Drake ◽  
Anna Bebenek
Keyword(s):  
Dna Polymerase ◽  
Replication Fidelity

scholarly journals The Enhanced DNA Replication Fidelity of a Mutant Herpes Simplex Virus Type 1 DNA Polymerase Is Mediated by an Improved Nucleotide Selectivity and Reduced Mismatch Extension Ability

2008 ◽  
Vol 82 (17) ◽  
pp. 8937-8941 ◽  
Author(s):  
Wang Tian ◽  
Ying T. Hwang ◽  
Charles B. C. Hwang
Keyword(s):  
Herpes Simplex Virus ◽  
Dna Replication ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Herpes Simplex Virus Type ◽  
Wild Type ◽  
Replication Fidelity ◽  
Simplex Virus ◽  
Dna Replication Fidelity

ABSTRACT We previously demonstrated that a recombinant herpes simplex virus containing a mutation within the finger domain of DNA polymerase replicated DNA with increased fidelity. In this study, we demonstrate that, compared with wild-type polymerase, the mutant enzyme exhibited improved nucleotide selectivity and a reduced ability to extend from mismatched primer termini, which would contribute to the increased DNA replication fidelity.

Mechanism of DNA replication fidelity for three mutants of DNA polymerase I: Klenow fragment KF(exo+), KF(polA5), and KF(exo-)

Biochemistry ◽  
1991 ◽  
Vol 30 (5) ◽  
pp. 1441-1448 ◽  
Author(s):  
Bryan T. Eger ◽  
Robert D. Kuchta ◽  
Steven S. Carroll ◽  
Patricia A. Benkovic ◽  
Michael E. Dahlberg ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Polymerase ◽  
Klenow Fragment ◽  
Dna Polymerase I ◽  
Replication Fidelity ◽  
Polymerase I ◽  
Dna Replication Fidelity

scholarly journals Palm Mutants in DNA Polymerases α and η Alter DNA Replication Fidelity and Translesion Activity

2004 ◽  
Vol 24 (7) ◽  
pp. 2734-2746 ◽  
Author(s):  
Atsuko Niimi ◽  
Siripan Limsirichaikul ◽  
Shonen Yoshida ◽  
Shigenori Iwai ◽  
Chikahide Masutani ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Pyrimidine Dimer ◽  
Replication Fidelity ◽  
Dna Polymerase Α ◽  
Replication Errors ◽  
Translesion Dna

ABSTRACT We isolated active mutants in Saccharomyces cerevisiae DNA polymerase α that were associated with a defect in error discrimination. Among them, L868F DNA polymerase α has a spontaneous error frequency of 3 in 100 nucleotides and 570-fold lower replication fidelity than wild-type (WT) polymerase α. In vivo, mutant DNA polymerases confer a mutator phenotype and are synergistic with msh2 or msh6, suggesting that DNA polymerase α-dependent replication errors are recognized and repaired by mismatch repair. In vitro, L868F DNA polymerase α catalyzes efficient bypass of a cis-syn cyclobutane pyrimidine dimer, extending the 3′ T 26,000-fold more efficiently than the WT. Phe34 is equivalent to residue Leu868 in translesion DNA polymerase η, and the F34L mutant of S. cerevisiae DNA polymerase η has reduced translesion DNA synthesis activity in vitro. These data suggest that high-fidelity DNA synthesis by DNA polymerase α is required for genomic stability in yeast. The data also suggest that the phenylalanine and leucine residues in translesion and replicative DNA polymerases, respectively, might have played a role in the functional evolution of these enzyme classes.

scholarly journals Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment

1997 ◽  
Vol 94 (15) ◽  
pp. 8042-8046 ◽  
Author(s):  
H. K. Dressman ◽  
C.-C. Wang ◽  
J. D. Karam ◽  
J. W. Drake
Keyword(s):  
Dna Polymerase ◽  
Replication Fidelity
Sign in / Sign up

Export Citation Format

Share Document