scholarly journals A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons

2007 ◽  
Vol 35 (17) ◽  
pp. e114 ◽  
Author(s):  
Anne-Laure Chateigner-Boutin ◽  
Ian Small
Keyword(s):  
High Resolution ◽  
Rna Editing ◽  
High Throughput ◽  
High Resolution Melting ◽  
High Throughput Method ◽  
Detection And Quantification

scholarly journals High Resolution Melting (HRM) for High-Throughput Genotyping—Limitations and Caveats in Practical Case Studies

2017 ◽  
Vol 18 (11) ◽  
pp. 2316 ◽  
Author(s):  
Marcin Słomka ◽  
Marta Sobalska-Kwapis ◽  
Monika Wachulec ◽  
Grzegorz Bartosz ◽  
Dominik Strapagiel
Keyword(s):  
High Resolution ◽  
Case Studies ◽  
High Throughput ◽  
High Resolution Melting ◽  
Practical Case

Detection and quantification of cashew in commercial tea products using High Resolution Melting (HRM) analysis

2020 ◽  
Vol 85 (6) ◽  
pp. 1629-1634 ◽  
Author(s):  
Georgios Lagiotis ◽  
Evangelia Stavridou ◽  
Irini Bosmali ◽  
Maslin Osathanunkul ◽  
Nadia Haider ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Melting ◽  
Hrm Analysis ◽  
Detection And Quantification

scholarly journals Development and Validation of High-Resolution Melting Markers Derived from Rysto STS Markers for High-Throughput Marker-Assisted Selection of Potato Carrying Rysto

2016 ◽  
Vol 106 (11) ◽  
pp. 1366-1375 ◽  
Author(s):  
Xianzhou Nie ◽  
Darcy Sutherland ◽  
Virginia Dickison ◽  
Mathuresh Singh ◽  
Agnes M. Murphy ◽  
...  
Keyword(s):  
High Resolution ◽  
High Throughput ◽  
Marker Assisted Selection ◽  
High Resolution Melting ◽  
Chromosome Region ◽  
Sts Markers ◽  
Reverse Primer ◽  
Selection For ◽  
Development And Validation ◽  
Selection Of

Sequence analysis of the chromosome region harboring the sequence-tagged site (STS) markers YES3-3A and YES3-3B for Rysto, a gene responsible for extreme resistance to Potato virus Y (PVY) in potato, was performed in tetraploid potato ‘Barbara’ (Rrrr) and ‘AC Chaleur’ (rrrr) as well as their progeny selections. Three and two sequence variants were identified in Barbara resistant (R) selections and AC Chaleur susceptible (S) selections, respectively. Further analysis indicates that the variant with a 21-nucleotide (nt) deletion is likely the chromosome copy harboring the STS markers. Two primer pairs, one targeting the region containing a 20-nt deletion and the other targeting the region anchoring the YES3-3A reverse primer, were designed. As anticipated, pair one produced two visible fragments in Barbara-R bulk and one visible fragment in AC Chaleur-S bulk; pair two produced one visible fragment in all samples. When subjected to high-resolution melting (HRM) analysis, two distinct melting profiles for R and S samples were observed. Analysis of 147 progeny of Barbara × AC Chaleur revealed 72 and 75 progeny with R and S melting profiles, respectively, which was consistent with YES3-3A and YES3-3B assays and phenotyping analysis, thus demonstrating the potential of HRM profiles as novel molecular markers for Rysto. The efficacy of the newly developed HRM markers for high-throughput marker-assisted selection for Rysto-conferred resistance to PVY was validated further with three populations involving Barbara as the R parent.

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