scholarly journals Effects of nucleic acid local structure and magnesium ions on minus-strand transfer mediated by the nucleic acid chaperone activity of HIV-1 nucleocapsid protein

2007 ◽  
Vol 35 (12) ◽  
pp. 3974-3987 ◽  
Author(s):  
T. Wu ◽  
S. L. Heilman-Miller ◽  
J. G. Levin
Keyword(s):  
Nucleic Acid ◽  
Local Structure ◽  
Nucleocapsid Protein ◽  
Chaperone Activity ◽  
Strand Transfer ◽  
Minus Strand ◽  
Magnesium Ions ◽  
Nucleic Acid Chaperone ◽  
Hiv 1

Rationally Designed Peptides as Efficient Inhibitors of Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein

Biochemistry ◽  
2018 ◽  
Vol 57 (30) ◽  
pp. 4562-4573 ◽  
Author(s):  
Volodymyr Shvadchak ◽  
Sarwat Zgheib ◽  
Beata Basta ◽  
Nicolas Humbert ◽  
Johannes Langedijk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleocapsid Protein ◽  
Chaperone Activity ◽  
Nucleic Acid Chaperone ◽  
Hiv 1

scholarly journals Actinomycin D Inhibits Human Immunodeficiency Virus Type 1 Minus-Strand Transfer in In Vitro and Endogenous Reverse Transcriptase Assays

1998 ◽  
Vol 72 (8) ◽  
pp. 6716-6724 ◽  
Author(s):  
Jianhui Guo ◽  
Tiyun Wu ◽  
Julian Bess ◽  
Louis E. Henderson ◽  
Judith G. Levin
Keyword(s):  
Human Immunodeficiency Virus ◽  
Nucleic Acid ◽  
Actinomycin D ◽  
Strand Transfer ◽  
Minus Strand ◽  
Nucleic Acid Chaperone ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT In this report we demonstrate that human immunodeficiency virus type 1 (HIV-1) minus-strand transfer, assayed in vitro and in endogenous reactions, is greatly inhibited by actinomycin D. Previously we showed that HIV-1 nucleocapsid (NC) protein (a nucleic acid chaperone catalyzing nucleic acid rearrangements which lead to more thermodynamically stable conformations) dramatically stimulates HIV-1 minus-strand transfer by preventing TAR-dependent self-priming from minus-strand strong-stop DNA [(−) SSDNA]. Despite this potent activity, the addition of NC to in vitro reactions with actinomycin D results in only a modest increase in the 50% inhibitory concentration (IC50) for the drug. PCR analysis of HIV-1 endogenous reactions indicates that minus-strand transfer is inhibited by the drug with an IC50 similar to that observed when NC is present in the in vitro system. Taken together, these results demonstrate that NC cannot overcome the inhibitory effect of actinomycin D on minus-strand transfer. Other experiments reveal that at actinomycin D concentrations which severely curtail minus-strand transfer, neither the synthesis of (−) SSDNA nor RNase H degradation of donor RNA is affected; however, the annealing of (−) SSDNA to acceptor RNA is significantly reduced. Thus, inhibition of the annealing reaction is responsible for actinomycin D-mediated inhibition of strand transfer. Since NC (but not reverse transcriptase) is required for efficient annealing, we conclude that actinomycin D inhibits minus-strand transfer by blocking the nucleic acid chaperone activity of NC. Our findings also suggest that actinomycin D, already approved for treatment of certain tumors, might be useful in combination therapy for AIDS.

scholarly journals Fidelity of plus-strand priming requires the nucleic acid chaperone activity of HIV-1 nucleocapsid protein

2009 ◽  
Vol 37 (6) ◽  
pp. 1755-1766 ◽  
Author(s):  
Klara Post ◽  
Besik Kankia ◽  
Swathi Gopalakrishnan ◽  
Victoria Yang ◽  
Elizabeth Cramer ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleocapsid Protein ◽  
Chaperone Activity ◽  
Nucleic Acid Chaperone ◽  
Hiv 1

scholarly journals Replacement of a Single Aromatic Residue in HIV-1 Nucleocapsid Protein Strongly Alters its Nucleic Acid Chaperone Activity

2010 ◽  
Vol 98 (3) ◽  
pp. 269a
Author(s):  
Hao Wu ◽  
Micah J. McCauley ◽  
Robert J. Gorelick ◽  
Ioulia Rouzina ◽  
Karin Musier-Forsyth ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleocapsid Protein ◽  
Chaperone Activity ◽  
Aromatic Residue ◽  
Nucleic Acid Chaperone ◽  
Hiv 1

Nucleic Acid Conformational Changes Essential for HIV-1 Nucleocapsid Protein-mediated Inhibition of Self-priming in Minus-strand Transfer

2003 ◽  
Vol 325 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Minh K Hong ◽  
Elizabeth J Harbron ◽  
Donald B O'Connor ◽  
Jianhui Guo ◽  
Paul F Barbara ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Conformational Changes ◽  
Nucleocapsid Protein ◽  
Strand Transfer ◽  
Minus Strand ◽  
Hiv 1

scholarly journals Mechanism for nucleic acid chaperone activity of HIV-1 nucleocapsid protein revealed by single molecule stretching

2001 ◽  
Vol 98 (11) ◽  
pp. 6121-6126 ◽  
Author(s):  
M. C. Williams ◽  
I. Rouzina ◽  
J. R. Wenner ◽  
R. J. Gorelick ◽  
K. Musier-Forsyth ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Single Molecule ◽  
Nucleocapsid Protein ◽  
Chaperone Activity ◽  
Nucleic Acid Chaperone ◽  
Hiv 1
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