scholarly journals Selection and cloning of poly(rC)-binding protein 2 and Raf kinase inhibitor protein RNA activators of 2′,5′-oligoadenylate synthetase from prostate cancer cells

2006 ◽  
Vol 34 (22) ◽  
pp. 6684-6695 ◽  
Author(s):  
Ross J. Molinaro ◽  
Babal Kant Jha ◽  
Krishnamurthy Malathi ◽  
Sooryanarayana Varambally ◽  
Arul M. Chinnaiyan ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Kinase Inhibitor ◽  
Binding Protein ◽  
Inhibitor Protein ◽  
Prostate Cancer Cells ◽  
Oligoadenylate Synthetase ◽  
Raf Kinase ◽  
Raf Kinase Inhibitor Protein

scholarly journals Phosphatidylethanolamine binding protein 1 enhances sensitivity of gastric cancer cell to 5-fluorouracil via inhibition of cell proliferation, migration and invasion

2021 ◽  
Vol 19 (12) ◽  
pp. 2583-2590
Author(s):  
Mengxin Lin ◽  
Xiaoyan Lin ◽  
Xiaobing Huang ◽  
Qing Liu ◽  
Riping Wu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Gastric Cancer Cell ◽  
Kinase Inhibitor ◽  
Binding Protein ◽  
Inhibitor Protein ◽  
Raf Kinase ◽  
Raf Kinase Inhibitor Protein ◽  
Phosphatidylethanolamine Binding Protein

Purpose: To determine the association between phosphatidylethanolamine binding protein 1, which is an Raf kinase inhibitor protein (RKIP), and 5-fluorouracil (5-FU) via analysis of the association between RKIP and clinical responses in individuals treated using fluorouracil-based chemotherapy.Methods: Human gastric cancer cell lines MGC-803 and SGC-7901 were used in this study. Cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis and migration were determined with flow cytometry and Transwell chamber assays, respectively. The mRNA and protein expressions of apoptosis-related factors were assayed using realtime polymerase chain reaction (RT-PCR) and Western blotting, respectively, while the expression of RKIP was determined by immunohistochemical staining.Results: Chemotherapeutic drug (5-FU) treatment induced low RKIP expression levels in tumorigenic GC cells, thereby sensitizing the cells to apoptosis (8.57 vs 1.25 %, p < 0.01). The highest RKIP level correlated well with initiation of apoptosis (4.20 vs 1.25 %, p < 0.01). Following in vitro downregulation of RKIP, there was increase in the viability and proliferation of RKIP-inhibited cells over time, and these changes were linked to alterations in cell cycle phases and increased optical density in MTT proliferation assay (1.55 vs 1.18, p < 0.01). In vitro Transwell assay measurement revealed an association between RKIP downregulation and enhancement of cell migration potential (652 vs 436, p < 0.01). Ectopic RKIP expression restored the apoptotic sensitivity of resistant cells (14.30 vs 1.36 %, p <0.01). This sensitization was annulled by upregulation of survival routes. Reduction of RKIP by expression of antisense and siRNA conferred resistance on cancer cells sensitive to 5-FU-mediated apoptosis (6.88 vs 2.13 %, p < 0.01).Conclusion: Thus, RKIP is a promising therapeutic strategy for improving the efficacy of clinically relevant chemotherapeutic drugs for GC. Keywords: Gastric cancer, Raf kinase inhibitor protein, Cell proliferation, Invasion, Apoptosis, Chemotherapy,  Phosphatidylethanolamine binding protein 1

scholarly journals Metastasis suppressor gene Raf kinase inhibitor protein (RKIP) is a novel prognostic marker in prostate cancer

The Prostate ◽  
2006 ◽  
Vol 66 (3) ◽  
pp. 248-256 ◽  
Author(s):  
Zheng Fu ◽  
Yasuhide Kitagawa ◽  
Ronglai Shen ◽  
Rajal Shah ◽  
Rohit Mehra ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prognostic Marker ◽  
Kinase Inhibitor ◽  
Suppressor Gene ◽  
Metastasis Suppressor ◽  
Metastasis Suppressor Gene ◽  
Inhibitor Protein ◽  
Raf Kinase ◽  
Raf Kinase Inhibitor Protein

scholarly journals Raf kinase inhibitor protein (RKIP) deficiency decreases latency of tumorigenesis and increases metastasis in a murine genetic model of prostate cancer

The Prostate ◽  
2014 ◽  
Vol 75 (3) ◽  
pp. 292-302 ◽  
Author(s):  
June Escara-Wilke ◽  
Jill M. Keller ◽  
Kathleen M. Woods Ignatoski ◽  
Jinlu Dai ◽  
Gregory Shelley ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Kinase Inhibitor ◽  
Genetic Model ◽  
Inhibitor Protein ◽  
Raf Kinase ◽  
Raf Kinase Inhibitor Protein
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