scholarly journals Sequence determinants of breakpoint location during HIV-1 intersubtype recombination

2006 ◽  
Vol 34 (18) ◽  
pp. 5203-5216 ◽  
Author(s):  
Heather A. Baird ◽  
Román Galetto ◽  
Yong Gao ◽  
Etienne Simon-Loriere ◽  
Measho Abreha ◽  
...  
Keyword(s):  
Intersubtype Recombination ◽  
Breakpoint Location ◽  
Hiv 1

scholarly journals An in vitro Model to Mimic Selection of Replication-Competent HIV-1 Intersubtype Recombination in Dual or Superinfected Patients

2017 ◽  
Vol 429 (14) ◽  
pp. 2246-2264 ◽  
Author(s):  
Bernard S. Bagaya ◽  
Meijuan Tian ◽  
Gabrielle C. Nickel ◽  
José F. Vega ◽  
Yuejin Li ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Intersubtype Recombination ◽  
Vitro Model ◽  
Hiv 1 ◽  
Selection Of

Determination of Gag and Env subtypes of HIV-1 detected among injecting drug users (IDUs) in Manipur, India: Evidence for intersubtype recombination

2005 ◽  
Vol 114 (1-2) ◽  
pp. 149-153 ◽  
Author(s):  
Payel Bhanja ◽  
Satarupa Sengupta ◽  
N. Yaima Singh ◽  
Kamalesh Sarkar ◽  
Sujit Kumar Bhattacharya ◽  
...  
Keyword(s):  
Drug Users ◽  
Injecting Drug Users ◽  
Intersubtype Recombination ◽  
Hiv 1

scholarly journals Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein

2010 ◽  
Vol 7 (1) ◽  
pp. 259 ◽  
Author(s):  
Cristian De Candia ◽  
Constanza Espada ◽  
Gabriel Duette ◽  
Yanina Ghiglione ◽  
Gabriela Turk ◽  
...  
Keyword(s):  
Viral Replication ◽  
Intersubtype Recombination ◽  
Hiv 1

scholarly journals Estimating the Rate of Intersubtype Recombination in Early HIV-1 Group M Strains

2012 ◽  
Vol 87 (4) ◽  
pp. 1967-1973 ◽  
Author(s):  
M. J. Ward ◽  
S. J. Lycett ◽  
M. L. Kalish ◽  
A. Rambaut ◽  
A. J. Leigh Brown
Keyword(s):  
Intersubtype Recombination ◽  
Hiv 1

scholarly journals Identification of a major restriction in HIV-1 intersubtype recombination

2005 ◽  
Vol 102 (25) ◽  
pp. 9002-9007 ◽  
Author(s):  
M. P. S. Chin ◽  
T. D. Rhodes ◽  
J. Chen ◽  
W. Fu ◽  
W.-S. Hu
Keyword(s):  
Intersubtype Recombination ◽  
Hiv 1

scholarly journals In Vitro Intersubtype Recombinants of Human Immunodeficiency Virus Type 1: Comparison to Recent and Circulating In Vivo Recombinant Forms

2002 ◽  
Vol 76 (19) ◽  
pp. 9600-9613 ◽  
Author(s):  
Miguel E. Quiñones-Mateu ◽  
Yong Gao ◽  
Sarah C. Ball ◽  
Andre J. Marozsan ◽  
Awet Abraha ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Intersubtype Recombination ◽  
Immunodeficiency Virus ◽  
The World ◽  
Hiv 1 ◽  
Dual Infections

ABSTRACT The increased prevalence of human immunodeficiency virus type 1 (HIV-1) intersubtype recombinants (ISRs) is shaping HIV-1 evolution throughout the world and will have an impact on both therapeutic and vaccine strategies. This study was designed to generate and compare in vitro ISRs to those isolated from HIV-infected individuals throughout the world. Human peripheral blood mononuclear cells were dually infected with seven pairs of HIV-1 isolates from different subtypes (i.e., A to F). Recombinant crossover sites were mapped to specific regions in the envelope (env) gene by using a cloning-hybridization technique and subtype-specific probes. In vitro intersubtype recombination was at least twofold more frequent in the V1-to-V3 region than in any other env fragment, i.e., C1 to V1, V3 to V5, or V5 to gp41. Sequence and recombination site analyses suggested the C2 env domain as a “hot region” for recombination and selection of replication-competent ISRs during the 15-day incubation. In addition to these regional preferences for env recombination, homopolymeric nucleotide tracts, i.e., sequences known to pause reverse transcriptase and promote template switching, were found in most in vitro crossover sites. ISRs, originating from recent dual infections and limited transmission events, partly retained this in vitro regional or sequence preference for recombination sites. However, a shift to crossover sites flanking the gp120-coding sequence was evident in the stable circulating recombinant forms of HIV-1. Based on these findings, HIV-1 recombinants generated from these dual infections may be used as a model for in vivo intersubtype recombination and for the design of various diagnostic assays and vaccine constructs.

scholarly journals Molecular determinants of HIV-1 intersubtype recombination potential

Virology ◽  
2007 ◽  
Vol 363 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Mario P.S. Chin ◽  
Jianbo Chen ◽  
Olga A. Nikolaitchik ◽  
Wei-Shau Hu
Keyword(s):  
Intersubtype Recombination ◽  
Molecular Determinants ◽  
Hiv 1

Ultrastructural observations of human monocytes infected with HIV-1

1991 ◽  
Vol 49 ◽  
pp. 108-109
Author(s):  
James K. Koehler ◽  
Steven G. Reed ◽  
Joao S. Silva
Keyword(s):  
Gradient Centrifugation ◽  
Virus Production ◽  
Human Monocyte ◽  
Red Cross ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Hiv Replication ◽  
Electron Microscopic ◽  
Ultrastructural Studies ◽  
Hiv 1

As part of a larger study involving the co-infection of human monocyte cultures with HIV and protozoan parasites, electron microscopic observations were made on the course of HIV replication and infection in these cells. Although several ultrastructural studies of the cytopathology associated with HIV infection have appeared, few studies have shown the details of virus production in “normal,” human monocytes/macrophages, one of the natural targets of the virus, and suspected of being a locus of quiescent virus during its long latent period. In this report, we detail some of the interactions of developing virons with the membranes and organelles of the monocyte host.Peripheral blood monocytes were prepared from buffy coats (Portland Red Cross) by Percoll gradient centrifugation, followed by adherence to cover slips. 90-95% pure monocytes were cultured in RPMI with 5% non-activated human AB serum for four days and infected with 100 TCID50/ml of HIV-1 for four hours, washed and incubated in fresh medium for 14 days.

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