scholarly journals A simple DNA stretching method for fluorescence imaging of single DNA molecules

2006 ◽  
Vol 34 (17) ◽  
pp. e113-e113 ◽  
Author(s):  
Ting-Fung Chan ◽  
Connie Ha ◽  
Angie Phong ◽  
Dongmei Cai ◽  
Eunice Wan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Dna Molecules ◽  
Dna Stretching ◽  
Single Dna Molecules

scholarly journals Nanochannel-Confined TAMRA-Polypyrrole Stained DNA Stretching by Varying the Ionic Strength from Micromolar to Millimolar Concentrations

Polymers ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 15 ◽  
Author(s):  
Seonghyun Lee ◽  
Yelin Lee ◽  
Yongkyun Kim ◽  
Cong Wang ◽  
Jungyul Park ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Double Helix ◽  
Persistence Length ◽  
Polymer Physics ◽  
Structural Deformation ◽  
Contour Length ◽  
Dna Molecules ◽  
Dna Stretching ◽  
Single Dna Molecules ◽  
Length Changes

Large DNA molecules have been utilized as a model system to investigate polymer physics. However, DNA visualization via intercalating dyes has generated equivocal results due to dye-induced structural deformation, particularly unwanted unwinding of the double helix. Thus, the contour length increases and the persistence length changes so unpredictably that there has been a controversy. In this paper, we used TAMRA-polypyrrole to stain single DNA molecules. Since this staining did not change the contour length of B-form DNA, we utilized TAMRA-polypyrrole stained DNA as a tool to measure the persistence length by changing the ionic strength. Then, we investigated DNA stretching in nanochannels by varying the ionic strength from 0.06 mM to 47 mM to evaluate several polymer physics theories proposed by Odijk, de Gennes and recent papers to deal with these regimes.

Photobleaching of Asymmetric Cyanines Used for Fluorescence Imaging of Single DNA Molecules

2001 ◽  
Vol 123 (33) ◽  
pp. 7985-7995 ◽  
Author(s):  
Claire Kanony ◽  
Björn Åkerman ◽  
Eimer Tuite
Keyword(s):  
Fluorescence Imaging ◽  
Dna Molecules ◽  
Single Dna Molecules

scholarly journals Stretching DNA to twice the normal length with single-molecule hydrodynamic trapping

Lab on a Chip ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 1780-1791
Author(s):  
Yan Jiang ◽  
Theodore Feldman ◽  
Julia A. M. Bakx ◽  
Darren Yang ◽  
Wesley P. Wong
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
High Speed ◽  
Force Spectroscopy ◽  
Dna Molecules ◽  
Normal Length ◽  
Single Dna Molecules ◽  
Hydrodynamic Trapping

High-speed hydrodynamic trapping enables combined surface-free force spectroscopy and fluorescence imaging of single DNA molecules at extreme forces.

Thermoplastic Nanofluidic Devices for Identifying Abasic Sites in Single DNA Molecules

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Steven A Soper ◽  
Swarnagowri Vaidyanathan ◽  
Franklin Uba ◽  
Bo Hu ◽  
David Kaufman ◽  
...  
Keyword(s):  
Dna Damage ◽  
Therapeutic Efficacy ◽  
Double Strand Breaks ◽  
Dna Molecules ◽  
Strand Breaks ◽  
Single Dna Molecules ◽  
Abasic Sites ◽  
Nanofluidic Devices ◽  
Ap Sites

DNA damage can take many forms such as double-strand breaks and/or the formation of abasic (apurinic/apyrimidinic; AP) sites. The presence of AP sites can be used to determine therapeutic efficacy...

scholarly journals Active Delivery of Single DNA Molecules into a Plasmonic Nanopore for Label-Free Optical Sensing

Nano Letters ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 8003-8010 ◽  
Author(s):  
Xin Shi ◽  
Daniel V. Verschueren ◽  
Cees Dekker
Keyword(s):  
Optical Sensing ◽  
Label Free ◽  
Dna Molecules ◽  
Single Dna Molecules
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