scholarly journals Development of a species-specific RNA polymerase I-based shRNA expression vector

2006 ◽  
Vol 35 (2) ◽  
pp. e10-e10 ◽  
Author(s):  
M. S. Brenz Verca ◽  
P. Weber ◽  
C. Mayer ◽  
C. Graf ◽  
D. Refojo ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Expression Vector ◽  
Rna Polymerase I ◽  
Shrna Expression ◽  
Shrna Expression Vector ◽  
Species Specific ◽  
Polymerase I

scholarly journals Species-specific rDNA transcription is due to promoter-specific binding factors.

1984 ◽  
Vol 4 (2) ◽  
pp. 221-227 ◽  
Author(s):  
R Miesfeld ◽  
N Arnheim
Keyword(s):  
Rna Polymerase ◽  
Transcriptional Control ◽  
Specific Binding ◽  
Rna Polymerase I ◽  
Rdna Transcription ◽  
Nucleolar Dominance ◽  
Cell Extracts ◽  
Species Specific ◽  
Polymerase I

RNA polymerase I transcription factors were purified from HeLa and mouse L cell extracts by phosphocellulose chromatography. Three fractions from each species were found to be required for transcription. One of these fractions, virtually devoid of RNA polymerase I activity, was found to form a stable preinitiation complex with small DNA fragments containing promoter sequences from the homologous but not the heterologous species. These species-specific DNA-binding factors can explain nucleolar dominance in vivo in mouse-human hybrid somatic cells and species specificity in cell-free, RNA polymerase I-dependent transcription systems. The evolution of species-specific transcriptional control signals may be the natural outcome of a special relationship that exists between the RNA polymerase I transcription machinery and the multigene family coding for rRNA.

In vitro transcription of plant RNA polymerase I-dependent rRNA genes is species-specific

1995 ◽  
Vol 8 (2) ◽  
pp. 295-298 ◽  
Author(s):  
Hao Fan ◽  
Kimitaka Yakura ◽  
Masako Miyanishi ◽  
Mamoru Sugita ◽  
Masahiro Sugiura
Keyword(s):  
Rna Polymerase ◽  
In Vitro Transcription ◽  
Rna Polymerase I ◽  
Rrna Genes ◽  
Species Specific ◽  
Polymerase I

Species-specific rDNA transcription is due to promoter-specific binding factors

1984 ◽  
Vol 4 (2) ◽  
pp. 221-227
Author(s):  
R Miesfeld ◽  
N Arnheim
Keyword(s):  
Rna Polymerase ◽  
Transcriptional Control ◽  
Specific Binding ◽  
Rna Polymerase I ◽  
Rdna Transcription ◽  
Nucleolar Dominance ◽  
Cell Extracts ◽  
Species Specific ◽  
Polymerase I

RNA polymerase I transcription factors were purified from HeLa and mouse L cell extracts by phosphocellulose chromatography. Three fractions from each species were found to be required for transcription. One of these fractions, virtually devoid of RNA polymerase I activity, was found to form a stable preinitiation complex with small DNA fragments containing promoter sequences from the homologous but not the heterologous species. These species-specific DNA-binding factors can explain nucleolar dominance in vivo in mouse-human hybrid somatic cells and species specificity in cell-free, RNA polymerase I-dependent transcription systems. The evolution of species-specific transcriptional control signals may be the natural outcome of a special relationship that exists between the RNA polymerase I transcription machinery and the multigene family coding for rRNA.

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