scholarly journals Characterization of the Type III restriction endonuclease PstII from Providencia stuartii

2005 ◽  
Vol 33 (15) ◽  
pp. 4775-4787 ◽  
Author(s):  
A. Sears ◽  
L. J. Peakman ◽  
G. G. Wilson ◽  
M. D. Szczelkun
Keyword(s):  
Restriction Endonuclease ◽  
Type Iii ◽  
Providencia Stuartii

scholarly journals Characterization of the Type III restriction endonuclease PstII from Providencia stuartii

2019 ◽  
Vol 47 (21) ◽  
pp. 11477-11479
Author(s):  
Alice Sears ◽  
Luke J Peakman ◽  
Geoffrey G Wilson ◽  
Mark D Szczelkun
Keyword(s):  
Restriction Endonuclease ◽  
Type Iii ◽  
Providencia Stuartii

Characterization of Partial Gene Deletions in Type III von Willebrand Disease with Alloantibody Inhibitors

1994 ◽  
Vol 72 (02) ◽  
pp. 180-185 ◽  
Author(s):  
David J Mancuso ◽  
Elodee A Tuley ◽  
Ricardo Castillo ◽  
Norma de Bosch ◽  
Pler M Mannucci ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Von Willebrand Disease ◽  
Pcr Analysis ◽  
Gene Deletions ◽  
Factor Gene ◽  
Type Iii ◽  
Large Deletions ◽  
Von Willebrand ◽  
Willebrand Factor

Summaryvon Willebrand factor gene deletions were characterized in four patients with severe type III von Willebrand disease and alloantibodies to von Willebrand factor. A PCR-based strategy was used to characterize the boundaries of the deletions. Identical 30 kb von Willebrand factor gene deletions which include exons 33 through 38 were identified in two siblings of one family by this method. A small 5 base pair insertion (CCTGG) was sequenced at the deletion breakpoint. PCR analysis was used to detect the deletion in three generations of the family, including two family members who are heterozygous for the deletion. In a second family, two type III vWD patients, who are distant cousins, share an -56 kb deletion of exons 22 through 43. The identification and characterization of large vWF gene deletions in these type III vWD patients provides further support for the association between large deletions in both von Willebrand factor alleles and the development of inhibitory alloantibodies.

Establishment and characterization of hamster cell lines transformed by restriction endonuclease fragments of adenovirus 5.

1984 ◽  
Vol 49 (1) ◽  
pp. 162-170 ◽  
Author(s):  
D T Rowe ◽  
P E Branton ◽  
S P Yee ◽  
S Bacchetti ◽  
F L Graham
Keyword(s):  
Restriction Endonuclease ◽  
Cell Lines ◽  
Adenovirus 5

scholarly journals Characterization of Phospholipase Activity of the Pseudomonas aeruginosa Type III Cytotoxin, ExoU

2005 ◽  
Vol 187 (3) ◽  
pp. 1192-1195 ◽  
Author(s):  
Hiromi Sato ◽  
Jimmy B. Feix ◽  
Cecilia J. Hillard ◽  
Dara W. Frank
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Substrate Specificity ◽  
Yeast Extract ◽  
Enzyme Activation ◽  
Phospholipase Activity ◽  
Eukaryotic Protein ◽  
Type Iii

ABSTRACT Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.

scholarly journals Crystallization and preliminary X-ray characterization of a type III cohesin–dockerin complex from the cellulosome system ofRuminococcus flavefaciens

2012 ◽  
Vol 68 (9) ◽  
pp. 1116-1119 ◽  
Author(s):  
Orly Salama-Alber ◽  
Yair Gat ◽  
Raphael Lamed ◽  
Linda J. W. Shimon ◽  
Edward A. Bayer ◽  
...  
Keyword(s):  
X Ray ◽  
Type Iii

Structural Domains in the Type III Restriction Endonuclease EcoP15I: Characterization by Limited Proteolysis, Mass Spectrometry and Insertional Mutagenesis

2007 ◽  
Vol 366 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Katja Wagenführ ◽  
Stefan Pieper ◽  
Petra Mackeldanz ◽  
Michael Linscheid ◽  
Detlev H. Krüger ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Restriction Endonuclease ◽  
Insertional Mutagenesis ◽  
Limited Proteolysis ◽  
Structural Domains ◽  
Type Iii
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