scholarly journals Novel DNA-binding properties of the RNA-binding protein TIAR

2005 ◽  
Vol 33 (14) ◽  
pp. 4507-4518 ◽  
Author(s):  
E. A. Suswam
Keyword(s):  
Dna Binding ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Binding Properties ◽  
Dna Binding Properties

scholarly journals C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation

2021 ◽  
Author(s):  
Yanzhou Zhang ◽  
Mahmoud Bassal ◽  
Daniel Friedrich ◽  
Simone Ummarino ◽  
Tom Verbiest ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Specific Gene ◽  
Macrophage Differentiation ◽  
Binding Domains ◽  
Rna Binding Domains ◽  
Neutrophil Differentiation

C/EBPα has known to be a transcription factor that involved in Neutrophil differentiation for decades. However, exploring the Chromatin RNA Immunoprecipitation Sequencing (RIP), we discover that C/EBPα is a RNA binding protein mainly interacts with RNA introns. Structure study and RNA electrophoretic mobility shift assay (REMSA) show that C/EBPα interacts with RNA through two novel RNA binding domains distinct from its DNA binding domain. Mouse bone marrow transplantation and in vitro cytokine assay reveal that C/EBPα RNA binding is critical for Macrophage differentiation but not Neutrophil differentiation. Mechanically, RNA binding domains control specific gene transcription. In particular, PU.1 intron 4 RNA interacts with C/EBPα and recruit C/EBPα to its enhancer site, which facilitate PU.1 expression. Taken together, C/EBPα is demonstrated to be a RNA binding protein with unique function distinct from its DNA binding activity. Our finding transforms our knowledge of transcriptional regulation by transcription factor.

DNA-binding properties of cloned TATA-binding protein from potato tubers

1992 ◽  
Vol 19 (3) ◽  
pp. 455-464 ◽  
Author(s):  
Michael J. Holdsworth ◽  
Claire Grierson ◽  
Wolfgang Schuch ◽  
Mike Bevan
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Tata Binding Protein ◽  
Potato Tubers ◽  
Binding Properties ◽  
Dna Binding Properties
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