scholarly journals The post-transcriptional gene silencing machinery functions independently of DNA methylation to repress a LINE1-like retrotransposon in Neurospora crassa

2005 ◽  
Vol 33 (5) ◽  
pp. 1564-1573 ◽  
Author(s):  
T. Nolan
Keyword(s):  
Dna Methylation ◽  
Gene Silencing ◽  
Neurospora Crassa ◽  
Transcriptional Gene Silencing ◽  
Post Transcriptional Gene Silencing

scholarly journals Viroids as a Tool to Study RNA-Directed DNA Methylation in Plants

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1187
Author(s):  
Michael Wassenegger ◽  
Athanasios Dalakouras
Keyword(s):  
Dna Methylation ◽  
Rna Interference ◽  
Gene Silencing ◽  
Plant Cells ◽  
Transcriptional Gene Silencing ◽  
Rnai Machinery ◽  
Double Stranded Rna ◽  
Post Transcriptional Gene Silencing ◽  
Cumulative Amount

Viroids are plant pathogenic, circular, non-coding, single-stranded RNAs (ssRNAs). Members of the Pospiviroidae family replicate in the nucleus of plant cells through double-stranded RNA (dsRNA) intermediates, thus triggering the host’s RNA interference (RNAi) machinery. In plants, the two RNAi pillars are Post-Transcriptional Gene Silencing (PTGS) and RNA-directed DNA Methylation (RdDM), and the latter has the potential to trigger Transcriptional Gene Silencing (TGS). Over the last three decades, the employment of viroid-based systems has immensely contributed to our understanding of both of these RNAi facets. In this review, we highlight the role of Pospiviroidae in the discovery of RdDM, expound the gradual elucidation through the years of the diverse array of RdDM’s mechanistic details and propose a revised RdDM model based on the cumulative amount of evidence from viroid and non-viroid systems.

scholarly journals Post-transcriptional gene silencing triggers dispensable DNA methylation in gene body in Arabidopsis

2019 ◽  
Vol 47 (17) ◽  
pp. 9104-9114 ◽  
Author(s):  
Christelle Taochy ◽  
Agnès Yu ◽  
Nicolas Bouché ◽  
Nathalie Bouteiller ◽  
Taline Elmayan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Silencing ◽  
De Novo ◽  
Transcriptional Silencing ◽  
Transcriptional Gene Silencing ◽  
Gene Body ◽  
Coding Sequence ◽  
Post Transcriptional Gene Silencing ◽  
Methylation Signal ◽  
Rule Out

Abstract Spontaneous post-transcriptional silencing of sense transgenes (S-PTGS) is established in each generation and is accompanied by DNA methylation, but the pathway of PTGS-dependent DNA methylation is unknown and so is its role. Here we show that CHH and CHG methylation coincides spatially and temporally with RDR6-dependent products derived from the central and 3′ regions of the coding sequence, and requires the components of the RNA-directed DNA methylation (RdDM) pathway NRPE1, DRD1 and DRM2, but not CLSY1, NRPD1, RDR2 or DCL3, suggesting that RDR6-dependent products, namely long dsRNAs and/or siRNAs, trigger PTGS-dependent DNA methylation. Nevertheless, none of these RdDM components are required to establish S-PTGS or produce a systemic silencing signal. Moreover, preventing de novo DNA methylation in non-silenced transgenic tissues grafted onto homologous silenced tissues does not inhibit the triggering of PTGS. Overall, these data indicate that gene body DNA methylation is a consequence, not a cause, of PTGS, and rule out the hypothesis that a PTGS-associated DNA methylation signal is transmitted independent of a PTGS signal.

RNA-DNA Interactions and DNA Methylation in Post-Transcriptional Gene Silencing

1999 ◽  
Vol 11 (12) ◽  
pp. 2291 ◽  
Author(s):  
Louise Jones ◽  
Andrew J. Hamilton ◽  
Olivier Voinnet ◽  
Carole L. Thomas ◽  
Andrew J. Maule ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Silencing ◽  
Transcriptional Gene Silencing ◽  
Dna Interactions ◽  
Post Transcriptional Gene Silencing

scholarly journals RNA–DNA Interactions and DNA Methylation in Post-Transcriptional Gene Silencing

1999 ◽  
Vol 11 (12) ◽  
pp. 2291-2301 ◽  
Author(s):  
Louise Jones ◽  
Andrew J. Hamilton ◽  
Olivier Voinnet ◽  
Carole L. Thomas ◽  
Andrew J. Maule ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Gene Silencing ◽  
Transcriptional Gene Silencing ◽  
Dna Interactions ◽  
Post Transcriptional Gene Silencing
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