scholarly journals Direct transcriptional repression of the genes encoding the zinc-finger proteins Gfi1b and Gfi1 by Gfi1b

2005 ◽  
Vol 33 (3) ◽  
pp. 987-998 ◽  
Author(s):  
L. Vassen
Keyword(s):  
Zinc Finger ◽  
Transcriptional Repression ◽  
Zinc Finger Proteins ◽  
Genes Encoding

scholarly journals KAP-1 Corepressor Protein Interacts and Colocalizes with Heterochromatic and Euchromatic HP1 Proteins: a Potential Role for Krüppel-Associated Box–Zinc Finger Proteins in Heterochromatin-Mediated Gene Silencing

1999 ◽  
Vol 19 (6) ◽  
pp. 4366-4378 ◽  
Author(s):  
Robert F. Ryan ◽  
David C. Schultz ◽  
Kasirajan Ayyanathan ◽  
Prim B. Singh ◽  
Josh R. Friedman ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Zinc Finger ◽  
Transcriptional Repression ◽  
Zinc Finger Proteins ◽  
Centromeric Heterochromatin ◽  
Interphase Nuclei ◽  
Epigenetic Gene Silencing ◽  
Associated Proteins

ABSTRACT Krüppel-associated box (KRAB) domains are present in approximately one-third of all human zinc finger proteins (ZFPs) and are potent transcriptional repression modules. We have previously cloned a corepressor for the KRAB domain, KAP-1, which is required for KRAB-mediated repression in vivo. To characterize the repression mechanism utilized by KAP-1, we have analyzed the ability of KAP-1 to interact with murine (M31 and M32) and human (HP1α and HP1γ) homologues of the HP1 protein family, a class of nonhistone heterochromatin-associated proteins with a well-established epigenetic gene silencing function in Drosophila. In vitro studies confirmed that KAP-1 is capable of directly interacting with M31 and hHP1α, which are normally found in centromeric heterochromatin, as well as M32 and hHP1γ, both of which are found in euchromatin. Mapping of the region in KAP-1 required for HP1 interaction showed that amino acid substitutions which abolish HP1 binding in vitro reduce KAP-1 mediated repression in vivo. We observed colocalization of KAP-1 with M31 and M32 in interphase nuclei, lending support to the biochemical evidence that M31 and M32 directly interact with KAP-1. The colocalization of KAP-1 with M31 is sometimes found in subnuclear territories of potential pericentromeric heterochromatin, whereas colocalization of KAP-1 and M32 occurs in punctate euchromatic domains throughout the nucleus. This work suggests a mechanism for the recruitment of HP1-like gene products by the KRAB-ZFP–KAP-1 complex to specific loci within the genome through formation of heterochromatin-like complexes that silence gene activity. We speculate that gene-specific repression may be a consequence of the formation of such complexes, ultimately leading to silenced genes in newly formed heterochromatic chromosomal environments.

scholarly journals Identification of genes encoding zinc finger proteins, non-histone chromosomal HMG protein homologue, and a putative GTP phosphohydrolase in the genome of Chilo iridescent virus

1994 ◽  
Vol 22 (2) ◽  
pp. 158-166 ◽  
Author(s):  
Paul Schnitzler ◽  
Michael Hug ◽  
Michaela Handermann ◽  
Waltraud Janssen ◽  
Eugene V. Koonin ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Chilo Iridescent Virus ◽  
Iridescent Virus ◽  
Genes Encoding ◽  
Hmg Protein

scholarly journals C2H2-Type Zinc Finger Proteins (DkZF1/2) Synergistically Control Persimmon Fruit Deastringency

2019 ◽  
Vol 20 (22) ◽  
pp. 5611
Author(s):  
Wajeeha Jamil ◽  
Wei Wu ◽  
Hui Gong ◽  
Jing-Wen Huang ◽  
Mudassar Ahmad ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Zinc Finger Proteins ◽  
Bimolecular Fluorescence Complementation ◽  
Luciferase Assay ◽  
Response Factors ◽  
High Co2 ◽  
Persimmon Fruit ◽  
Genes Encoding

Hypoxic environments are generally undesirable for most plants, but for astringent persimmon, high CO2 treatment (CO2 > 90%), also termed artificial high-CO2 atmosphere (AHCA), causes acetaldehyde accumulation and precipitation of soluble tannins and could remove astringency. The multiple transcriptional regulatory linkages involved in persimmon fruit deastringency have been advanced significantly by characterizing the ethylene response factors (ERFs), WRKY and MYB; however, the involvement of zinc finger proteins for deastringency has not been investigated. In this study, five genes encoding C2H2-type zinc finger proteins were isolated and designed as DkZF1-5. Phylogenetic and sequence analyses suggested the five DkZFs could be clustered into two different subgroups. qPCR analysis indicated that transcript abundances of DkZF1/4 were significantly upregulated during AHCA treatment (1% O2 and 95% CO2) at day 1, DkZF2/5 at both day 1 and 2, while DkZF3 at day 2. Dual-luciferase assay indicated DkZF1 and DkZF2 as the activators of deastringency-related structural genes (DkPDC2 and DkADH1) and transcription factors (DkERF9/10). Moreover, combinative effects between various transcription factors were investigated, indicating that DkZF1 and DkZF2 synergistically showed significantly stronger activations on the DkPDC2 promoter. Further, both bimolecular fluorescence complementation (BiFC) and yeast two hybrid (Y2H) assays confirmed that DkZF2 had protein–protein interactions with DkZF1. Thus, these findings illustrate the regulatory mechanisms of zinc finger proteins for persimmon fruit deastringency under AHCA.

Three Genes Encoding Zinc Finger Proteins on Human Chromosome 6p21.3: Members of a New Subclass of the Krüppel Gene Family Containing the Conserved SCAN Box Domain

Genomics ◽  
1997 ◽  
Vol 43 (2) ◽  
pp. 191-201 ◽  
Author(s):  
Pauline L. Lee ◽  
Terri Gelbart ◽  
Carol West ◽  
Michele Adams ◽  
Ryan Blackstone ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Gene Family ◽  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Genes Encoding ◽  
Chromosome 6P21.3

scholarly journals KRAB–Zinc Finger Proteins and KAP1 Can Mediate Long-Range Transcriptional Repression through Heterochromatin Spreading

PLoS Genetics ◽  
2010 ◽  
Vol 6 (3) ◽  
pp. e1000869 ◽  
Author(s):  
Anna C. Groner ◽  
Sylvain Meylan ◽  
Angela Ciuffi ◽  
Nadine Zangger ◽  
Giovanna Ambrosini ◽  
...  
Keyword(s):  
Long Range ◽  
Zinc Finger ◽  
Transcriptional Repression ◽  
Zinc Finger Proteins

scholarly journals The Kruppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression.

1994 ◽  
Vol 91 (10) ◽  
pp. 4514-4518 ◽  
Author(s):  
R. Witzgall ◽  
E. O'Leary ◽  
A. Leaf ◽  
D. Onaldi ◽  
J. V. Bonventre
Keyword(s):  
Zinc Finger ◽  
Transcriptional Repression ◽  
Zinc Finger Proteins

KAP1-independent transcriptional repression of SCAN-KRAB-containing zinc finger proteins

2009 ◽  
Vol 388 (4) ◽  
pp. 689-694 ◽  
Author(s):  
Yasuhide Itokawa ◽  
Toshihide Yanagawa ◽  
Hisashi Yamakawa ◽  
Naoko Watanabe ◽  
Hisashi Koga ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Transcriptional Repression ◽  
Zinc Finger Proteins

Cloning and characterization of two yeast genes encoding members of the CCCH class of zinc finger proteins: zinc finger-mediated impairment of cell growth

Gene ◽  
1996 ◽  
Vol 174 (2) ◽  
pp. 225-233 ◽  
Author(s):  
Michael J. Thompson ◽  
Wi S. Lai ◽  
Gregory A. Taylor ◽  
Perry J. Blackshear
Keyword(s):  
Cell Growth ◽  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Genes Encoding ◽  
Yeast Genes
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