scholarly journals Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene

2004 ◽  
Vol 32 (22) ◽  
pp. 6716-6716
Author(s):  
E. A. Worthey
Keyword(s):  
Leishmania Major ◽  
Trna Gene ◽  
Gene Clusters ◽  
Chromosome 3

scholarly journals Transcription Initiation and Termination on Leishmania major Chromosome 3

2004 ◽  
Vol 3 (2) ◽  
pp. 506-517 ◽  
Author(s):  
Santiago Martínez-Calvillo ◽  
Dan Nguyen ◽  
Kenneth Stuart ◽  
Peter J. Myler
Keyword(s):  
Rna Polymerase Ii ◽  
Gene Cluster ◽  
Transcription Initiation ◽  
Leishmania Major ◽  
Trna Gene ◽  
Gene Clusters ◽  
Chromosome 1 ◽  
Gene Region ◽  
Transcription Terminator ◽  
The Right

ABSTRACT Genome projects involving Leishmania and other trypanosomatids have revealed that most genes in these organisms are organized into large clusters of genes on the same DNA strand. We have previously shown that transcription of the entire Leishmania major Friedlin (LmjF) chromosome 1 (chr1) initiates bidirectionally between two divergent gene clusters. Here, we analyze transcription of LmjF chr3, which contains two convergent clusters of 67 and 30 genes, separated by a tRNA gene, with a single divergent protein-coding gene located close to the “left” telomere. Nuclear run-on analyses indicate that specific transcription of chr3 initiates bidirectionally between the single subtelomeric gene and the adjacent 67-gene cluster, close to the “right” telomere upstream of the 30-gene cluster, and upstream of the tRNA gene. Transcription on both strands terminates within the tRNA-gene region. Transient-transfection studies support the role of the tRNA-gene region as a transcription terminator for RNA polymerase II (Pol II) and Pol III, and also for Pol I.

scholarly journals Base J represses genes at the end of polycistronic gene clusters in Leishmania major by promoting RNAP II termination

2016 ◽  
Vol 101 (4) ◽  
pp. 559-574 ◽  
Author(s):  
David L. Reynolds ◽  
Brigitte T. Hofmeister ◽  
Laura Cliffe ◽  
T. Nicolai Siegel ◽  
Britta A. Anderson ◽  
...  
Keyword(s):  
Leishmania Major ◽  
Gene Clusters ◽  
Rnap Ii

Structure and in vitro transcription of tRNA gene clusters containing the primers of MuLV reverse transcriptase

1986 ◽  
Vol 158 (3) ◽  
pp. 437-442 ◽  
Author(s):  
Tommaso RUSSO ◽  
Francesco COSTANZO ◽  
Adriana OLIVA ◽  
Rosario AMMENDOLA ◽  
Angela DUILIO ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Trna Gene ◽  
Gene Clusters ◽  
In Vitro Transcription

scholarly journals Global In-Silico Scenario of tRNA Genes and Their Organization in Virus Genomes

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 180 ◽  
Author(s):  
Sergio Morgado ◽  
Ana Vicente
Keyword(s):  
In Silico ◽  
Large Scale ◽  
Trna Gene ◽  
Gene Clusters ◽  
Trna Genes ◽  
Genome Survey ◽  
In Silico Study ◽  
Domains Of Life ◽  
Dsdna Viruses ◽  
Virus Genomes

Viruses are known to be highly dependent on the host translation machinery for their protein synthesis. However, tRNA genes are occasionally identified in such organisms, and in addition, few of them harbor tRNA gene clusters comprising dozens of genes. Recently, tRNA gene clusters have been shown to occur among the three domains of life. In such a scenario, the viruses could play a role in the dispersion of such structures among these organisms. Thus, in order to reveal the prevalence of tRNA genes as well as tRNA gene clusters in viruses, we performed an unbiased large-scale genome survey. Interestingly, tRNA genes were predicted in ssDNA (single-stranded DNA) and ssRNA (single-stranded RNA) viruses as well in many other dsDNA viruses of families from Caudovirales order. In the latter group, tRNA gene clusters composed of 15 to 37 tRNA genes were characterized, mainly in bacteriophages, enlarging the occurrence of such structures within viruses. These bacteriophages were from hosts that encompass five phyla and 34 genera. This in-silico study presents the current global scenario of tRNA genes and their organization in virus genomes, contributing and opening questions to be explored in further studies concerning the role of the translation apparatus in these organisms.

scholarly journals Regulation of transcription termination by glucosylated hydroxymethyluracil, base J, in Leishmania major and Trypanosoma brucei

2014 ◽  
Vol 42 (15) ◽  
pp. 9717-9729 ◽  
Author(s):  
David Reynolds ◽  
Laura Cliffe ◽  
Konrad U. Förstner ◽  
Chung-Chau Hon ◽  
T. Nicolai Siegel ◽  
...  
Keyword(s):  
Cell Death ◽  
Trypanosoma Brucei ◽  
Leishmania Major ◽  
Nuclear Dna ◽  
Epigenetic Modification ◽  
Transcription Termination ◽  
Gene Clusters ◽  
Regulation Of Transcription ◽  
Genome Wide ◽  
Rnap Ii

Abstract Base J, β-d-glucosyl-hydroxymethyluracil, is an epigenetic modification of thymine in the nuclear DNA of flagellated protozoa of the order Kinetoplastida. J is enriched at sites involved in RNA polymerase (RNAP) II initiation and termination. Reduction of J in Leishmania tarentolae via growth in BrdU resulted in cell death and indicated a role of J in the regulation of RNAP II termination. To further explore J function in RNAP II termination among kinetoplastids and avoid indirect effects associated with BrdU toxicity and genetic deletions, we inhibited J synthesis in Leishmania major and Trypanosoma brucei using DMOG. Reduction of J in L. major resulted in genome-wide defects in transcription termination at the end of polycistronic gene clusters and the generation of antisense RNAs, without cell death. In contrast, loss of J in T. brucei did not lead to genome-wide termination defects; however, the loss of J at specific sites within polycistronic gene clusters led to altered transcription termination and increased expression of downstream genes. Thus, J regulation of RNAP II transcription termination genome-wide is restricted to Leishmania spp., while in T. brucei it regulates termination and gene expression at specific sites within polycistronic gene clusters.

scholarly journals Exploring tRNA gene cluster in archaea

2018 ◽  
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Gene Cluster ◽  
Trna Gene ◽  
Gene Clusters ◽  
Gene Organization ◽  
Trna Genes ◽  
Genomic Analyses ◽  
Living Organisms

AbstractShared traits between prokaryotes and eukaryotes are helpful in the understanding of the tree of life evolution. In bacteria and eukaryotes, it has been shown a particular organization of tRNA genes as clusters, but this trait has not been explored in archaea domain. Here, based on analyses of complete and draft archaeal genomes, we demonstrated the prevalence of tRNA gene clusters in archaea. tRNA gene cluster was identified at least in three Archaea class, Halobacteria, Methanobacteria and Methanomicrobia from Euryarchaeota supergroup. Genomic analyses also revealed evidence of tRNA gene cluster associated with mobile genetic elements and horizontal gene transfer inter/intra-domain. The presence of tRNA gene clusters in the three domain of life suggests a role of this type of tRNA gene organization in the biology of the living organisms.

scholarly journals Nucleotide sequences of two tRNA gene clusters fromMicrococcus luteus

1990 ◽  
Vol 18 (23) ◽  
pp. 7155-7155 ◽  
Author(s):  
Ryoji Ikeda ◽  
Takeshi Ohama ◽  
Akira Muto ◽  
Syozo Osawa
Keyword(s):  
Trna Gene ◽  
Gene Clusters ◽  
Nucleotide Sequences
Sign in / Sign up

Export Citation Format

Share Document