scholarly journals Type II restriction endonuclease R.KpnI is a member of the HNH nuclease superfamily

2004 ◽  
Vol 32 (20) ◽  
pp. 6129-6135 ◽  
Author(s):  
Matheshwaran Saravanan ◽  
Janusz M. Bujnicki ◽  
Iwona A. Cymerman ◽  
Desirazu N. Rao ◽  
Valakunja Nagaraja
Keyword(s):  
Restriction Endonuclease ◽  
Type Ii

scholarly journals Fsel, a new type II restriction endonuclease that recognizes the octanucleotide sequence 5′ GGCCGGCC 3′

1990 ◽  
Vol 18 (8) ◽  
pp. 2061-2064 ◽  
Author(s):  
Janise Meyertons Nelson ◽  
Sheila M. Miceli ◽  
Mary P. Lechevalier ◽  
Richard J. Roberts
Keyword(s):  
Restriction Endonuclease ◽  
Type Ii ◽  
New Type

Isolation and characterization of a newly identified type II restriction endonuclease from a local streptomyces sp. in Taiwan

1998 ◽  
Vol 73 (2-3) ◽  
pp. 231-241
Author(s):  
Kuang-Yu Hu ◽  
Jia-An Wuu ◽  
Ming-Ching Kao ◽  
Yu-Tien Liu ◽  
Shou-Hsiung Pai
Keyword(s):  
Restriction Endonuclease ◽  
Type Ii ◽  
Streptomyces Sp ◽  
Isolation And Characterization

scholarly journals A unique type II restriction endonuclease fromAcinetobacter twoffiN

1987 ◽  
Vol 15 (17) ◽  
pp. 7201-7201 ◽  
Author(s):  
Richard D. Morgan ◽  
Michael Dalton ◽  
Robert Stote
Keyword(s):  
Restriction Endonuclease ◽  
Type Ii ◽  
Unique Type

Covalent joining of the subunits of a homodimeric type II restriction endonuclease: single-chain PvuII endonuclease

2001 ◽  
Vol 309 (1) ◽  
pp. 89-97 ◽  
Author(s):  
András Simoncsits ◽  
Marie-Louise Tjörnhammar ◽  
Tamás Raskó ◽  
Antal Kiss ◽  
Sándor Pongor
Keyword(s):  
Restriction Endonuclease ◽  
Type Ii ◽  
Single Chain

scholarly journals A novel restriction endonuclease UnbI, a neoschizomer of Sau96I from an unidentified psychrofilic bacterium from Antarctica is inhibited by phosphate ions.

1997 ◽  
Vol 44 (4) ◽  
pp. 849-852 ◽  
Author(s):  
M Kawalec ◽  
P Borsuk ◽  
S Piechula ◽  
P P Stepień
Keyword(s):  
Low Temperature ◽  
Restriction Endonuclease ◽  
Bacterial Strain ◽  
Inorganic Phosphate ◽  
Restriction Enzymes ◽  
Type Ii ◽  
Temperature Optimum ◽  
Phosphate Ions ◽  
Sticky Ends

A novel type II restriction endonuclease UnbI was isolated from an unidentified psychrofilic bacterial strain from Antarctica. UnbI recognizes and cleaves the sequence 5'-GGNCC-3', producing 5 nucleotide long sticky ends. In this respect it differs from its neoschizomer Sau96I and all other restriction enzymes recognizing this sequence. UnbI has a relatively low temperature optimum of 15 degrees C to 20 degrees C and its activity is completely inhibited by inorganic phosphate.

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