scholarly journals Regulation of the large ( 1000 kb) imprinted murine Ube3a antisense transcript by alternative exons upstream of Snurf/Snrpn

2004 ◽  
Vol 32 (11) ◽  
pp. 3480-3492 ◽  
Author(s):  
M. Landers
Keyword(s):  
Antisense Transcript ◽  
Ube3a Antisense

scholarly journals CRISPR/Cas9 directed to the Ube3a antisense transcript improves Angelman syndrome phenotype in mice

2021 ◽  
Author(s):  
Ralf S. Schmid ◽  
Xuefeng Deng ◽  
Priyalakshmi Panikker ◽  
Msema Msackyi ◽  
Camilo Breton ◽  
...  
Keyword(s):  
Angelman Syndrome ◽  
Antisense Transcript ◽  
Ube3a Antisense

scholarly journals A bipartite boundary element restrictsUBE3Aimprinting to mature neurons

2019 ◽  
Vol 116 (6) ◽  
pp. 2181-2186 ◽  
Author(s):  
Jack S. Hsiao ◽  
Noelle D. Germain ◽  
Andrea Wilderman ◽  
Christopher Stoddard ◽  
Luke A. Wojenski ◽  
...  
Keyword(s):  
Boundary Element ◽  
Antisense Transcript ◽  
Neurodevelopmental Disorder ◽  
Paternal Allele ◽  
Loss Of Function ◽  
Gene Encoding ◽  
Specific Expression ◽  
Human Neurons ◽  
Mature Neurons ◽  
Ube3a Antisense

Angelman syndrome (AS) is a severe neurodevelopmental disorder caused by the loss of function from the maternal allele ofUBE3A, a gene encoding an E3 ubiquitin ligase.UBE3Ais only expressed from the maternally inherited allele in mature human neurons due to tissue-specific genomic imprinting. Imprinted expression ofUBE3Ais restricted to neurons by expression ofUBE3A antisense transcript(UBE3A-ATS) from the paternally inherited allele, which silences the paternal allele ofUBE3Aincis. However, the mechanism restrictingUBE3A-ATSexpression andUBE3Aimprinting to neurons is not understood. We used CRISPR/Cas9-mediated genome editing to functionally define a bipartite boundary element critical for neuron-specific expression ofUBE3A-ATSin humans. Removal of this element led to up-regulation ofUBE3A-ATSwithout repressing paternalUBE3A. However, increasing expression ofUBE3A-ATSin the absence of the boundary element resulted in full repression of paternalUBE3A, demonstrating thatUBE3Aimprinting requires both the loss of function from the boundary element as well as the up-regulation ofUBE3A-ATS. These results suggest that manipulation of the competition betweenUBE3A-ATSandUBE3Amay provide a potential therapeutic approach for AS.

scholarly journals Natural Antisense Transcript PEBP1P3 Regulates the RNA Expression, DNA Methylation and Histone Modification of CD45 Gene

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 759
Author(s):  
Zhongjing Su ◽  
Guangyu Liu ◽  
Bin Zhang ◽  
Ze Lin ◽  
Dongyang Huang
Keyword(s):  
Dna Methylation ◽  
Alternative Splicing ◽  
Histone Modification ◽  
Antisense Rna ◽  
Noncoding Rna ◽  
Antisense Transcript ◽  
Natural Antisense Transcript ◽  
Regulation Of Expression ◽  
Splicing Isoforms ◽  
Intron 2

The leukocyte common antigen CD45 is a transmembrane phosphatase expressed on all nucleated hemopoietic cells, and the expression levels of its splicing isoforms are closely related to the development and function of lymphocytes. PEBP1P3 is a natural antisense transcript from the opposite strand of CD45 intron 2 and is predicted to be a noncoding RNA. The genotype-tissue expression and quantitative PCR data suggested that PEBP1P3 might be involved in the regulation of expression of CD45 splicing isoforms. To explore the regulatory mechanism of PEBP1P3 in CD45 expression, DNA methylation and histone modification were detected by bisulfate sequencing PCR and chromatin immunoprecipitation assays, respectively. The results showed that after the antisense RNA PEBP1P3 was knocked down by RNA interference, the DNA methylation of CD45 intron 2 was decreased and histone H3K9 and H3K36 trimethylation at the alternative splicing exons of CD45 DNA was increased. Knockdown of PEBP1P3 also increased the binding levels of chromatin conformation organizer CTCF at intron 2 and the alternative splicing exons of CD45. The present results indicate that the natural antisense RNA PEBP1P3 regulated the alternative splicing of CD45 RNA, and that might be correlated with the regulation of histone modification and DNA methylation.

Long noncoding RNA FGFR3-AS1 promotes osteosarcoma growth through regulating its natural antisense transcript FGFR3

2016 ◽  
Vol 43 (5) ◽  
pp. 427-436 ◽  
Author(s):  
Jiabing Sun ◽  
Xuming Wang ◽  
Chunjiang Fu ◽  
Xiaoyu Wang ◽  
Jilong Zou ◽  
...  
Keyword(s):  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Antisense Transcript ◽  
Natural Antisense Transcript

P4-264: A GENE THERAPY APPROACH FOR REDUCTION OF TAU BY TAU LONG NON-CODING RNA GENE (MAPT-AS1 ) NATURAL ANTISENSE TRANSCRIPT

2006 ◽  
Vol 14 (7S_Part_29) ◽  
pp. P1547-P1548
Author(s):  
Rohan de Silva ◽  
Justyna Zareba-Paslawska ◽  
Filipa Almeida ◽  
Per Svenningsson ◽  
Tom Warner ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Antisense Transcript ◽  
Natural Antisense Transcript ◽  
Therapy Approach ◽  
Gene Therapy Approach ◽  
Non Coding Rna ◽  
Long Non Coding Rna
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