scholarly journals The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1

2004 ◽  
Vol 32 (3) ◽  
pp. 977-988 ◽  
Author(s):  
D. A. Rasko
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genome Sequence ◽  
Large Plasmid ◽  
Metabolic Adaptations

scholarly journals Complete Genome Sequence of an Environmental Bacillus cereus Isolate Belonging to the Bacillus anthracis Clade

2020 ◽  
Vol 9 (47) ◽  
Author(s):  
Leonid M. Irenge ◽  
Bertrand Bearzatto ◽  
Jérôme Ambroise ◽  
Jean-Luc Gala
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Soil Sample ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virulence Gene ◽  
Large Plasmid ◽  
Content Type

ABSTRACT We report here the complete genome sequence of a Bacillus cereus isolate identified in a soil sample from Namibia. This isolate is closely related to the B. anthracis clade. While the plasmids (500 and 12 kb) carry no detectable B. anthracis virulence gene, the large plasmid shares a 50-kb continuous region similar to plasmid pXO1.

scholarly journals Fine-scale differentiation between Bacillus anthracis and Bacillus cereus group signatures in metagenome shotgun data

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5515 ◽  
Author(s):  
Robert A. Petit III ◽  
James M. Hogan ◽  
Matthew N. Ezewudo ◽  
Sandeep J. Joseph ◽  
Timothy D. Read
Keyword(s):  
New York ◽  
New York City ◽  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genome Sequence ◽  
York City ◽  
False Positive ◽  
Lethal Factor ◽  
Analysis Tool ◽  
Sequencing Errors

Background It is possible to detect bacterial species in shotgun metagenome datasets through the presence of only a few sequence reads. However, false positive results can arise, as was the case in the initial findings of a recent New York City subway metagenome project. False positives are especially likely when two closely related are present in the same sample. Bacillus anthracis, the etiologic agent of anthrax, is a high-consequence pathogen that shares >99% average nucleotide identity with Bacillus cereus group (BCerG) genomes. Our goal was to create an analysis tool that used k-mers to detect B. anthracis, incorporating information about the coverage of BCerG in the metagenome sample. Methods Using public complete genome sequence datasets, we identified a set of 31-mer signatures that differentiated B. anthracis from other members of the B. cereus group (BCerG), and another set which differentiated BCerG genomes (including B. anthracis) from other Bacillus strains. We also created a set of 31-mers for detecting the lethal factor gene, the key genetic diagnostic of the presence of anthrax-causing bacteria. We created synthetic sequence datasets based on existing genomes to test the accuracy of a k-mer based detection model. Results We found 239,503 B. anthracis-specific 31-mers (the Ba31 set), 10,183 BCerG 31-mers (the BCerG31 set), and 2,617 lethal factor k-mers (the lef31 set). We showed that false positive B. anthracis k-mers—which arise from random sequencing errors—are observable at high genome coverages of B. cereus. We also showed that there is a “gray zone” below 0.184× coverage of the B. anthracis genome sequence, in which we cannot expect with high probability to identify lethal factor k-mers. We created a linear regression model to differentiate the presence of B. anthracis-like chromosomes from sequencing errors given the BCerG background coverage. We showed that while shotgun datasets from the New York City subway metagenome project had no matches to lef31 k-mers and hence were negative for B. anthracis, some samples showed evidence of strains very closely related to the pathogen. Discussion This work shows how extensive libraries of complete genomes can be used to create organism-specific signatures to help interpret metagenomes. We contrast “specialist” approaches to metagenome analysis such as this work to “generalist” software that seeks to classify all organisms present in the sample and note the more general utility of a k-mer filter approach when taxonomic boundaries lack clarity or high levels of precision are required.

scholarly journals Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis

Nature ◽  
2003 ◽  
Vol 423 (6935) ◽  
pp. 87-91 ◽  
Author(s):  
Natalia Ivanova ◽  
Alexei Sorokin ◽  
Iain Anderson ◽  
Nathalie Galleron ◽  
Benjamin Candelon ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genome Sequence

scholarly journals Draft Genome Sequence of Bacillus cereus LA2007, a Human-Pathogenic Isolate Harboring Anthrax-Like Plasmids

2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Angela Pena-Gonzalez ◽  
Chung K. Marston ◽  
Luis M. Rodriguez-R ◽  
Cari B. Kolton ◽  
Julia Garcia-Diaz ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Genome Sequence ◽  
Genome Analysis ◽  
Draft Genome ◽  
Anthrax Toxin ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Pneumonia Case ◽  
Pathogenic Isolate

ABSTRACT We present the genome sequence of Bacillus cereus LA2007, a strain isolated in 2007 from a fatal pneumonia case in Louisiana. Sequence-based genome analysis revealed that LA2007 carries a plasmid highly similar to Bacillus anthracis pXO1, including the genes responsible for the production and regulation of anthrax toxin.

Complete genome sequence of the cold-active bacteriophage VMY22 from Bacillus cereus

Virus Genes ◽  
2016 ◽  
Vol 52 (3) ◽  
pp. 432-435 ◽  
Author(s):  
Kunhao Qin ◽  
Benxu Cheng ◽  
Shengting Zhang ◽  
Nan Wang ◽  
Yuan Fang ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Cold Active

scholarly journals Genome Sequence of a Bacillus anthracis Outbreak Strain from Zambia, 2011

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
N. Ohnishi ◽  
F. Maruyama ◽  
H. Ogawa ◽  
H. Kachi ◽  
S. Yamada ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Genome Sequence ◽  
Outbreak Strain

scholarly journals Complete Genome Sequence of Bacillus cereus Sensu Stricto VKM B-370, Isolated from the Silkworm Bombyx mori

2021 ◽  
Vol 10 (20) ◽  
Author(s):  
Emma G. Piligrimova ◽  
Rustam M. Buzikov ◽  
Olesya A. Kazantseva ◽  
Andrey M. Shadrin
Keyword(s):  
Bacillus Cereus ◽  
Bombyx Mori ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Sensu Stricto ◽  
Host Strain ◽  
Gram Positive ◽  
Content Type ◽  
Silkworm Bombyx Mori

ABSTRACT Bacillus cereus is a Gram-positive rod-shaped spore-forming bacterium widespread in different environmental niches. Here, we report the complete genome sequence of Bacillus cereus VKM B-370 from the All-Russian Collection of Microorganisms, the host strain for bacteriophages vB_BtS_B83, vB_BcM_Sam46, vB_BcM_Sam112, and Izhevsk.

scholarly journals Bovine Bacillus anthracis in Cameroon

2011 ◽  
Vol 77 (16) ◽  
pp. 5818-5821 ◽  
Author(s):  
Paola Pilo ◽  
Alexandra Rossano ◽  
Hamadou Bamamga ◽  
Souley Abdoulkadiri ◽  
Vincent Perreten ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Anthracis ◽  
Content Type ◽  
Western Africa

ABSTRACTBovineBacillus anthracisisolates from Cameroon were genetically characterized. They showed a strong homogeneity, and they belong, together with strains from Chad, to cluster Aβ, which appears to be predominant in western Africa. However, one strain that belongs to a newly defined clade (D) and cluster (D1) is penicillin resistant and shows certain phenotypes typical ofBacillus cereus.

scholarly journals The Complete Genome Sequence of Bacillus anthracis Ames “Ancestor”

2008 ◽  
Vol 191 (1) ◽  
pp. 445-446 ◽  
Author(s):  
Jacques Ravel ◽  
Lingxia Jiang ◽  
Scott T. Stanley ◽  
Mark R. Wilson ◽  
R. Scott Decker ◽  
...  
Keyword(s):  
United States ◽  
Bacillus Anthracis ◽  
Genome Sequence ◽  
Cold Storage ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
The United States ◽  
Pathogenic Bacterium ◽  
The Subject ◽  
The Ideal

ABSTRACT The pathogenic bacterium Bacillus anthracis has become the subject of intense study as a result of its use in a bioterrorism attack in the United States in September and October 2001. Previous studies suggested that B. anthracis Ames Ancestor, the original Ames fully virulent plasmid-containing isolate, was the ideal reference. This study describes the complete genome sequence of that original isolate, derived from a sample kept in cold storage since 1981.

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