scholarly journals Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica

2004 ◽  
Vol 32 (1) ◽  
pp. 287-297 ◽  
Author(s):  
O. Fisher
Keyword(s):  
Entamoeba Histolytica ◽  
Dna Methyltransferase ◽  
Protozoan Parasite

scholarly journals Purification and molecular characterization of the NAD+-dependent acetaldehyde/alcohol dehydrogenase from Entamoeba histolytica

1994 ◽  
Vol 303 (3) ◽  
pp. 743-748 ◽  
Author(s):  
I Bruchhaus ◽  
E Tannich
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Dna Sequences ◽  
Microscopic Analysis ◽  
Kinetic Studies ◽  
Protozoan Parasite ◽  
Striking Similarity ◽  
Electron Microscopic ◽  
Catalytic Functions

A bifunctional 95 kDa polypeptide (EhADH2) harbouring acetaldehyde dehydrogenase and alcohol dehydrogenase activities was purified to homogeneity from trophozoite extracts of the protozoan parasite Entamoeba histolytica. Kinetic studies revealed that the enzyme utilizes NAD+ rather than NADP+ as cofactor. Km values for acetyl-CoA, acetaldehyde and ethanol were found to be 0.015, 0.15 and 80 mM respectively in the presence of 0.2 mM NAD+. The primary structure of EhADH2 as deduced from respective amoebic DNA sequences showed striking similarity to the trifunctional AdhE protein of Escherichia coli and the bifunctional AAD protein of Clostridium acetobutylicum. Alignment with a number of aldehyde dehydrogenases and alcohol dehydrogenases from various species suggested that the two catalytic functions of EhADH2 are located on separate parts of the molecule. By cross-linking experiments and electron-microscopic analysis, native EhADH2 was found to be organized in a homopolymeric fashion consisting of more than 20 associated promoters which form rods about 50-120 nm in length.

scholarly journals Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica

2005 ◽  
Vol 4 (4) ◽  
pp. 827-831 ◽  
Author(s):  
Mami Okada ◽  
Christopher D. Huston ◽  
Barbara J. Mann ◽  
William A. Petri ◽  
Kiyoshi Kita ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Proteomic Analysis ◽  
Protozoan Parasite ◽  
Immunofluorescence Assay ◽  
Vesicular Trafficking ◽  
Surface Recognition ◽  
Identification And Characterization ◽  
Enteric Protozoan ◽  
Cytoskeleton Rearrangement

ABSTRACT Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis.

Biochemical and functional characterization of novel NADH kinase in the enteric protozoan parasite Entamoeba histolytica

Biochimie ◽  
2013 ◽  
Vol 95 (2) ◽  
pp. 309-319 ◽  
Author(s):  
Ghulam Jeelani ◽  
Afzal Husain ◽  
Dan Sato ◽  
Tomoyoshi Soga ◽  
Makoto Suematsu ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Functional Characterization ◽  
Protozoan Parasite ◽  
Enteric Protozoan ◽  
Nadh Kinase

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

scholarly journals Identification and Characterization of the First Virulent Phages, Including a Novel Jumbo Virus, Infecting Ochrobactrum spp.

2020 ◽  
Vol 21 (6) ◽  
pp. 2096
Author(s):  
Przemyslaw Decewicz ◽  
Piotr Golec ◽  
Mateusz Szymczak ◽  
Monika Radlinska ◽  
Lukasz Dziewit
Keyword(s):  
Dna Methyltransferase ◽  
Wastewater Treatment Plants ◽  
Biogas Production ◽  
Genome Mining ◽  
Sewage Sample ◽  
Bacterial Strains ◽  
Regulatory Circuits ◽  
A Genome ◽  
Insightful Analysis

The Ochrobactrum genus consists of an extensive repertoire of biotechnologically valuable bacterial strains but also opportunistic pathogens. In our previous study, a novel strain, Ochrobactrum sp. POC9, which enhances biogas production in wastewater treatment plants (WWTPs) was identified and thoroughly characterized. Despite an insightful analysis of that bacterium, its susceptibility to bacteriophages present in WWTPs has not been evaluated. Using raw sewage sample from WWTP and applying the enrichment method, two virulent phages, vB_OspM_OC and vB_OspP_OH, which infect the POC9 strain, were isolated. These are the first virulent phages infecting Ochrobactrum spp. identified so far. Both phages were subjected to thorough functional and genomic analyses, which allowed classification of the vB_OspM_OC virus as a novel jumbo phage, with a genome size of over 227 kb. This phage encodes DNA methyltransferase, which mimics the specificity of cell cycle regulated CcrM methylase, a component of the epigenetic regulatory circuits in Alphaproteobacteria. In this study, an analysis of the overall diversity of Ochrobactrum-specific (pro)phages retrieved from databases and extracted in silico from bacterial genomes was also performed. Complex genome mining allowed us to build similarity networks to compare 281 Ochrobactrum-specific viruses. Analyses of the obtained networks revealed a high diversity of Ochrobactrum phages and their dissimilarity to the viruses infecting other bacteria.

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