scholarly journals The malaria parasite Plasmodium falciparum encodes members of the Puf RNA-binding protein family with conserved RNA binding activity

2002 ◽  
Vol 30 (21) ◽  
pp. 4607-4617 ◽  
Author(s):  
L. Cui
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Parasite ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Binding Activity ◽  
Protein Family ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum

scholarly journals Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome Maintenance Complex Binding Protein

2021 ◽  
Vol 9 (11) ◽  
pp. 2306
Author(s):  
Benjamin Liffner ◽  
Sabrina Absalon
Keyword(s):  
Plasmodium Falciparum ◽  
Nuclear Envelope ◽  
Malaria Parasite ◽  
Binding Protein ◽  
Mitotic Spindles ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
The Individual ◽  
Chromatin Bridges

The malaria parasite Plasmodium falciparum undergoes closed mitosis, which occurs within an intact nuclear envelope, and differs significantly from its human host. Mitosis is underpinned by the dynamics of microtubules and the nuclear envelope. To date, our ability to study P. falciparum mitosis by microscopy has been hindered by the small size of the P. falciparum nuclei. Ultrastructure expansion microscopy (U-ExM) has recently been developed for P. falciparum, allowing the visualization of mitosis at the individual nucleus level. Using U-ExM, three intranuclear microtubule structures are observed: hemispindles, mitotic spindles, and interpolar spindles. A previous study demonstrated that the mini-chromosome maintenance complex binding-protein (MCMBP) depletion caused abnormal nuclear morphology and microtubule defects. To investigate the role of microtubules following MCMBP depletion and study the nuclear envelope in these parasites, we developed the first nuclear stain enabled by U-ExM in P. falciparum. MCMBP-deficient parasites show aberrant hemispindles and mitotic spindles. Moreover, anaphase chromatin bridges and individual nuclei containing multiple microtubule structures were observed following MCMBP knockdown. Collectively, this study refines our understanding of MCMBP-deficient parasites and highlights the utility of U-ExM coupled with a nuclear envelope stain for studying mitosis in P. falciparum.

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