scholarly journals Highly conserved modified nucleosides influence Mg2+-dependent tRNA folding

2002 ◽  
Vol 30 (21) ◽  
pp. 4751-4760 ◽  
Author(s):  
K. N. Nobles
Keyword(s):  
Modified Nucleosides ◽  
Trna Folding

scholarly journals A One-Pot Approach to Novel Pyridazine C-Nucleosides

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2341
Author(s):  
Flavio Cermola ◽  
Serena Vella ◽  
Marina DellaGreca ◽  
Angela Tuzi ◽  
Maria Rosaria Iesce
Keyword(s):  
Organic Chemistry ◽  
Research Field ◽  
Modified Nucleosides ◽  
Protecting Groups ◽  
Coupling Reactions ◽  
One Pot ◽  
Pharmacological Interest ◽  
Classical Methodology ◽  
Glycosyl Donor ◽  
Neutral Conditions

The synthesis of glycosides and modified nucleosides represents a wide research field in organic chemistry. The classical methodology is based on coupling reactions between a glycosyl donor and an acceptor. An alternative strategy for new C-nucleosides is used in this approach, which consists of modifying a pre-existent furyl aglycone. This approach is applied to obtain novel pyridazine C-nucleosides starting with 2- and 3-(ribofuranosyl)furans. It is based on singlet oxygen [4+2] cycloaddition followed by reduction and hydrazine cyclization under neutral conditions. The mild three-step one-pot procedure leads stereoselectively to novel pyridazine C-nucleosides of pharmacological interest. The use of acetyls as protecting groups provides an elegant direct route to a deprotected new pyridazine C-nucleoside.

scholarly journals Modified Nucleosides, Nucleotides and Nucleic Acids via Click Azide-Alkyne Cycloaddition for Pharmacological Applications

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3100
Author(s):  
Daniela Perrone ◽  
Elena Marchesi ◽  
Lorenzo Preti ◽  
Maria Luisa Navacchia
Keyword(s):  
Cancer Therapy ◽  
Nucleic Acids ◽  
Click Chemistry ◽  
Nucleoside Analogs ◽  
Modified Nucleosides ◽  
Biological Molecules ◽  
Dipolar Cycloaddition ◽  
Virus Diseases ◽  
Reaction Conditions ◽  
Hybrid Compounds

The click azide = alkyne 1,3-dipolar cycloaddition (click chemistry) has become the approach of choice for bioconjugations in medicinal chemistry, providing facile reaction conditions amenable to both small and biological molecules. Many nucleoside analogs are known for their marked impact in cancer therapy and for the treatment of virus diseases and new targeted oligonucleotides have been developed for different purposes. The click chemistry allowing the tolerated union between units with a wide diversity of functional groups represents a robust means of designing new hybrid compounds with an extraordinary diversity of applications. This review provides an overview of the most recent works related to the use of click chemistry methodology in the field of nucleosides, nucleotides and nucleic acids for pharmacological applications.

scholarly journals Single-nucleotide control of tRNA folding cooperativity under near-cellular conditions

2019 ◽  
Vol 116 (46) ◽  
pp. 23075-23082
Author(s):  
Kathleen A. Leamy ◽  
Ryota Yamagami ◽  
Neela H. Yennawar ◽  
Philip C. Bevilacqua
Keyword(s):  
Rna Folding ◽  
3D Structure ◽  
Single Nucleotide ◽  
Transfer Rnas ◽  
E Coli ◽  
Posttranscriptional Processing ◽  
Structural Levels ◽  
Trna Folding ◽  
Precursor Transcript

RNA folding is often studied by renaturing full-length RNA in vitro and tracking folding transitions. However, the intracellular transcript folds as it emerges from the RNA polymerase. Here, we investigate the folding pathways and stability of numerous late-transcriptional intermediates of yeast and Escherichia coli transfer RNAs (tRNAs). Transfer RNA is a highly regulated functional RNA that undergoes multiple steps of posttranscriptional processing and is found in very different lengths during its lifetime in the cell. The precursor transcript is extended on both the 5′ and 3′ ends of the cloverleaf core, and these extensions get trimmed before addition of the 3′-CCA and aminoacylation. We studied the thermodynamics and structures of the precursor tRNA and of late-transcriptional intermediates of the cloverleaf structure. We examined RNA folding at both the secondary and tertiary structural levels using multiple biochemical and biophysical approaches. Our findings suggest that perhaps nature has selected for a single-base addition to control folding to the functional 3D structure. In near-cellular conditions, yeast tRNAPhe and E. coli tRNAAla transcripts fold in a single, cooperative transition only when nearly all of the nucleotides in the cloverleaf are transcribed by indirectly enhancing folding cooperativity. Furthermore, native extensions on the 5′ and 3′ ends do not interfere with cooperative core folding. This highly controlled cooperative folding has implications for recognition of tRNA by processing and modification enzymes and quality control of tRNA in cells.

scholarly journals Hypoxia-Selective DNA Interstrand Cross-Link Formation by Two Modified Nucleosides

2012 ◽  
Vol 18 (40) ◽  
pp. 12609-12613 ◽  
Author(s):  
Yunyan Kuang ◽  
Huabing Sun ◽  
J. Craig Blain ◽  
Xiaohua Peng
Keyword(s):  
Modified Nucleosides ◽  
Cross Link ◽  
Link Formation

Preferences of AAA/AAG codon recognition by modified nucleosides, τm5s2U34 and t6A37 present in tRNALys

2017 ◽  
Vol 36 (16) ◽  
pp. 4182-4196 ◽  
Author(s):  
Kailas D. Sonawane ◽  
Asmita S. Kamble ◽  
Prayagraj M. Fandilolu
Keyword(s):  
Modified Nucleosides ◽  
Codon Recognition

Ribose-Modified Nucleosides as Ligands for Adenosine Receptors:  Synthesis, Conformational Analysis, and Biological Evaluation of 1‘-C-Methyl Adenosine Analogues

2002 ◽  
Vol 45 (6) ◽  
pp. 1196-1202 ◽  
Author(s):  
Loredana Cappellacci ◽  
Grazia Barboni ◽  
Micaela Palmieri ◽  
Michela Pasqualini ◽  
Mario Grifantini ◽  
...  
Keyword(s):  
Conformational Analysis ◽  
Adenosine Receptors ◽  
Biological Evaluation ◽  
Modified Nucleosides ◽  
Adenosine Analogues
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