scholarly journals Molecular cloning and nucleotide sequence of the human growth hormone structural gene

1979 ◽  
Vol 7 (2) ◽  
pp. 305-320 ◽  
Author(s):  
W.G. Roskam ◽  
F. Rougeon
Keyword(s):  
Growth Hormone ◽  
Nucleotide Sequence ◽  
Molecular Cloning ◽  
Structural Gene ◽  
Human Growth Hormone ◽  
Human Growth

scholarly journals Molecular cloning, heterological expression and production of the human growth hormone

2019 ◽  
Vol 55 (2) ◽  
pp. 182-187
Author(s):  
A. M. Gorkavaya ◽  
A. A. Gilep ◽  
G. V. Sergeev
Keyword(s):  
Growth Hormone ◽  
Cell Proliferation ◽  
Molecular Cloning ◽  
Human Growth Hormone ◽  
Anterior Pituitary ◽  
Bacterial Culture ◽  
Recombinant Human Growth Hormone ◽  
Human Growth ◽  
Biochemical Pathways ◽  
His Tag

Growth hormone (GH) is a polypeptide produced in the anterior pituitary lobe that triggers different biochemical pathways and increases cell proliferation and growth. In this work, the pNic based vector for periplasmatic secretion was developed. Recombinant human growth hormone was produced via periplasmatic secretion (42h, 30 °С) followed by several steps of purification. GH obtained does not contain His-tag. Expression level of 2,85 mg per1 literof bacterial culture was achieved.

Molecular cloning of a teleost growth hormone receptor and its functional interaction with human growth hormone

Gene ◽  
2001 ◽  
Vol 270 (1-2) ◽  
pp. 121-129 ◽  
Author(s):  
Leo T.O Lee ◽  
G Nong ◽  
Y.H Chan ◽  
Dicky L.Y Tse ◽  
Christopher H.K Cheng
Keyword(s):  
Growth Hormone ◽  
Molecular Cloning ◽  
Human Growth Hormone ◽  
Hormone Receptor ◽  
Growth Hormone Receptor ◽  
Human Growth ◽  
Functional Interaction

The human growth hormone locus: Nucleotide sequence, biology, and evolution

Genomics ◽  
1989 ◽  
Vol 4 (4) ◽  
pp. 479-497 ◽  
Author(s):  
Ellson Y. Chen ◽  
Yu-Cheng Liao ◽  
Douglas H. Smith ◽  
Hugo A. Barrera-Saldaña ◽  
Richard E. Gelinas ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Nucleotide Sequence ◽  
Human Growth Hormone ◽  
Human Growth

Molecular cloning of cDNA encoding 20 kDa variant human growth hormone and the alternative splicing mechanism

1988 ◽  
Vol 949 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Naoki Masuda ◽  
Masanori Watahiki ◽  
Minoru Tanaka ◽  
Minoru Yamakawa ◽  
Ken Shimizu ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Alternative Splicing ◽  
Molecular Cloning ◽  
Human Growth Hormone ◽  
Human Growth

LIPOLYTIC ACTIVITY OF ENZYME DIGESTED HUMAN GROWTH HORMONE

1965 ◽  
Vol 49 (3_Suppl) ◽  
pp. S143
Author(s):  
Zvi Laron ◽  
Avivah Kowadlo-Silbergeld
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Lipolytic Activity ◽  
Human Growth

The minimal amino acid sequence of the insulin-potentiating fragments of human growth hormone: its mechanism of action

Diabetes ◽  
1980 ◽  
Vol 29 (10) ◽  
pp. 782-787 ◽  
Author(s):  
F. M. Ng ◽  
J. Bornstein ◽  
C. E. Pullin ◽  
J. O. Bromley ◽  
S. L. Macaulay
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Human Growth Hormone ◽  
Mechanism Of Action ◽  
Human Growth
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