scholarly journals The non-histone proteins of the rat liver nucleus and their distribution amongst chromatin fractions as produced by nuclease digestion

1979 ◽  
Vol 7 (1) ◽  
pp. 31-48 ◽  
Author(s):  
John E. Hyde ◽  
Tibor Igo-Kemenes ◽  
Hans G. Zachau
Keyword(s):  
Rat Liver ◽  
Liver Nucleus ◽  
Histone Proteins ◽  
Nuclease Digestion

scholarly journals Preferential Phosphorylation of Basic Non-histone Proteins by Nuclear Protein Kinase NII from Rat Liver

1980 ◽  
Vol 109 (2) ◽  
pp. 349-357 ◽  
Author(s):  
Tadayoshi HASUMA ◽  
Munehiko YUKIOKA ◽  
Satoshi NAKAJIMA ◽  
Seiji MORISAWA ◽  
Akira INOUE
Keyword(s):  
Protein Kinase ◽  
Rat Liver ◽  
Nuclear Protein ◽  
Histone Proteins

A comparison of the effect of lead nitrate on rat liver chromatin, DNA and histone proteins in solution

2008 ◽  
Vol 83 (6) ◽  
pp. 565-570 ◽  
Author(s):  
Azra Rabbani-Chadegani ◽  
Sayeh Abdosamadi ◽  
Nesa Fani ◽  
Shayesteh Mohammadian
Keyword(s):  
Rat Liver ◽  
Lead Nitrate ◽  
Histone Proteins ◽  
Liver Chromatin

scholarly journals Sequential changes in rat liver nuclear tri-iodothyronine receptors and mitochondrial α-glycerophosphate dehydrogenase activity after administeration of tri-iodothyronine

1979 ◽  
Vol 182 (2) ◽  
pp. 377-382 ◽  
Author(s):  
Hirotoshi Nakamura ◽  
Satoshi Hamada ◽  
Hiroo Imura
Keyword(s):  
Dehydrogenase Activity ◽  
Nuclear Receptors ◽  
Nuclear Receptor ◽  
Rat Liver ◽  
Time Course ◽  
Delayed Response ◽  
Histone Proteins ◽  
Glycerophosphate Dehydrogenase ◽  
Receptor Concentration ◽  
Hormonal Modulation

The dynamics of the induction of nuclear tri-iodothyronine receptors and mitochondrial α-glycerophosphate dehydrogenase were studied in rat liver after a single injection of tri-iodothyronine. The maximal binding capacity (Cmax.) and association constant (Ka) of the nuclear receptors were determined by Scatchard analyses with and without correction for the endogenous tri-iodothyronine measured by radioimmunoassay. The administration of tri-iodothyronine induced sequential increases in the concentration of nuclear receptors and α-glycerophosphate dehydrogenase activity in the liver. The nuclear-receptor concentration was increased to 2.5 times that in the hypothyroid rat 1 day after the administration of hormone, and then decreased, with a half-life of about 2 days. α-Glycerophosphate dehydrogenase activity changed in parallel with the nuclear-receptor concentration, showing a delayed response. The total amount of non-histone protein in the liver was significantly increased 3 days after the administration. It seems likely therefore that the tri-iodothyronine-induced increase in nuclear-receptor concentration is responsible, at least in part, for the induction of this enzyme. The possibility is also suggested that nuclear receptors may be one of the non-histone proteins selectively synthesized at an early stage of the hormonal stimulation. Throughout the time course, the Ka values of the nuclear receptors for tri-iodothyronine remained unchanged, when corrected for endogenous tri-iodothyronine bound to the non-histone proteins, although they were apparently changed when the correction was not made. The results obtained provide further evidence for hormonal modulation of the nuclear receptors which is closely linked with the hormonal effect.

scholarly journals Hemoglobin Formation in the Rat Liver Nucleus.

1955 ◽  
Vol 9 ◽  
pp. 1045-1045 ◽  
Author(s):  
R. Bonnichsen ◽  
G. Hevesy ◽  
John Glomset ◽  
Albertina Caglieris
Keyword(s):  
Rat Liver ◽  
Liver Nucleus

Differential Effects of Growth Hormone and Hydrocortisone on Nuclear RNA Synthesis in Rat Liver

1971 ◽  
Vol 49 (7) ◽  
pp. 853-862 ◽  
Author(s):  
Peter M. K. Ip ◽  
Maurice Brossard
Keyword(s):  
Growth Hormone ◽  
Rat Liver ◽  
Rna Synthesis ◽  
Present Report ◽  
Liver Nucleus ◽  
Double Labeling ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna ◽  
Liver Nuclei ◽  
Nucleolar Rna

RNA has been isolated and fractionated from rat liver nuclei by a sequence of salt extractions into four subfractions: namely nucleoplasmic, deoxyribonucleoprotein-associated, ribonucleoprotein-associated, and nucleolar fractions.Study of 14C-methyl-methionine labeling indicates that the nucleolar RNA fraction isolated by the present procedure is, in fact, of nucleolar origin while the other three fractions are essentially of extranucleolar origin.The effects of growth hormone and hydrocortisone on the nuclear RNA synthesis have been further studied using the present isolation and double-labeling techniques. Both hormones cause an increase in the incorporation of labeled orotic acid into all types of RNA in all four nuclear subfractions. However, the stimulatory effect of growth hormone is found mainly in the 18–28 S and 45–60 S regions of the nucleolar fraction and in regions of the entire gradient of the nucleoplasmic fraction, whereas the stimulatory effect of hydrocortisone is localized mainly in the 10–28 S regions of the nucleolar fraction and in the 4 S and 10–18 S regions of the ribonucleoprotein fraction.The present report suggests that there are qualitative as well as quantitative differences in the action of growth hormone and hydrocortisone on the synthesis of RNA in liver nucleus. The mode of action of the two hormones is discussed.

Tumour suppressor protein p53 released by nuclease digestion increases at the onset of rat liver regeneration

1999 ◽  
Vol 31 (2) ◽  
pp. 306-314 ◽  
Author(s):  
Francesc Miró ◽  
Jean-Claude Lelong ◽  
Floria Pancetti ◽  
Nerea Roher ◽  
Arlette Duthu ◽  
...  
Keyword(s):  
Liver Regeneration ◽  
Rat Liver ◽  
Tumour Suppressor ◽  
Rat Liver Regeneration ◽  
Protein P53 ◽  
Tumour Suppressor Protein ◽  
Nuclease Digestion
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