scholarly journals Physical map of the seven ribosomal RNA genes of Escherichia coli

1979 ◽  
Vol 6 (5) ◽  
pp. 1817-1830 ◽  
Author(s):  
Imre Boros ◽  
Antal Kiss ◽  
Phá Venetianer
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Physical Map ◽  
Ribosomal Rna Genes ◽  
Rna Genes

scholarly journals Physical map of the seven ribosomal RNA genes of Escherichia coli

1979 ◽  
Vol 6 (8) ◽  
pp. 2961-2961 ◽  
Author(s):  
I. Boros ◽  
A. Kiss ◽  
P. Venetianer
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Physical Map ◽  
Ribosomal Rna Genes ◽  
Rna Genes

In vitro Transcription of Escherichia coli Ribosomal RNA Genes

Nature ◽  
1972 ◽  
Vol 235 (5337) ◽  
pp. 329-333 ◽  
Author(s):  
WILLIAM A. HASELTINE
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
In Vitro Transcription ◽  
Ribosomal Rna Genes ◽  
Rna Genes

Study of the function of Escherichia coli ribosomal RNA through site-directed mutagenesis

1990 ◽  
Vol 68 (1) ◽  
pp. 169-179 ◽  
Author(s):  
Daniel Leclerc ◽  
Léa Brakier-Gingras
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Ribosomal Proteins ◽  
Large Body ◽  
Ribosomal Rna Genes ◽  
16S Ribosomal Rna ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Rna Genes

Various approaches have been developed to study how mutations in Escherichia coli ribosomal RNA affect the function of the ribosome. Most of them are in vivo approaches, where mutations are introduced in a specialized plasmid harboring the ribosomal RNA genes. The mutated plasmids are then expressed in an appropriate host, where they can confer resistance to antibiotics whose target is the ribosome. Conditions can be used where the host ribosomal RNA genes or the host ribosomes are selectively inactivated, and the effect of the mutations on ribosome assembly and function can be studied. Another approach, which has been developed mainly with 16S ribosomal RNA, can be used entirely in vitro. In this approach, a plasmid has been constructed which contains the 16S ribosomal RNA gene under control of a T7 promoter. Mutations can be introduced in the 16S ribosomal RNA sequence and the mutated 16S ribosomal RNAs are produced by in vitro transcription. It is then possible to investigate how the mutations affect the assembly of the 16S ribosomal RNA into 30S subunits and the activity of the reconstituted 30S subunits in cell-free protein synthesis assays. Although these approaches are recent, they have already provided a large body of interesting information, relating specific RNA sequences to interactions with ribosomal proteins, to ribosome function, and to its response to antibiotics.Key words: ribosomal RNA, ribosome, site-directed mutagenesis, antibiotic resistance.

Tandem duplications resulting from recombination between ribosomal RNA genes in Escherichia coli

1977 ◽  
Vol 116 (3) ◽  
pp. 407-428 ◽  
Author(s):  
C.W. Hill ◽  
Robert H. Grafstrom ◽  
Barbara Wallis Harnish ◽  
Barry S. Hillman
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rna Genes ◽  
Tandem Duplications
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