scholarly journals The localization of tRNA4Glugenes from Drosophila melanogaster by “in situ” hybridization

1978 ◽  
Vol 5 (5) ◽  
pp. 1465-1478 ◽  
Author(s):  
Eric Kubli ◽  
Thomas Schmidt
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster

1977 ◽  
Vol 115 (3) ◽  
pp. 539-563 ◽  
Author(s):  
Paul Szabo ◽  
Robert Elder ◽  
Dale M. Steffensen ◽  
Olke C. Uhlenbeck
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes

Transformation of Drosophila melanogaster with a suppressor tRNA gene from Schizosaccharomyces pombe

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Charles M. Molnar ◽  
Tove Reece ◽  
James A. Williams ◽  
John B. Bell
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
In Situ Hybridization ◽  
Schizosaccharomyces Pombe ◽  
Southern Hybridization ◽  
Trna Gene ◽  
P Element ◽  
Suppressor Trna ◽  
Good Agreement

P-element mediated transformation was utilized to introduce a suppressor tRNA gene [Formula: see text] from Schizosaccharomyces pombe into Drosophila melanogaster. Thirteen independently transformed lines were characterized as to the number and cytological locations of the transposons. It was ascertained that the suppressor tRNA gene of interest was introduced into each transformed strain. The helper P element used (pπ25.1) allows further transposition to occur, and it was determined that from one to seven copies of the heterologous [Formula: see text] gene per strain were present among the respective transformed strains. The number of transposons per transformed line was established by in situ hybridization to salivary gland chromosomes as well as by Southern hybridization analyses and there was good agreement in the totals determined by these two techniques.Key words: Drosophila, Schizosaccharomyces, tRNA suppressor, transformation, transposon.

HCR RNA-FISH protocol for the whole-mount brains of Drosophila and other insects v1

2021 ◽  
Author(s):  
Amanda A. G. Ferreira ◽  
Bogdan Sieriebriennikov ◽  
Hunter Whitbeck
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Alexa Fluor ◽  
Rna Fish ◽  
Whole Mount

This is a protocol to perform RNA fluorescent in situ hybridization (RNA-FISH) using hybridization chain reaction (HCR) on whole-mount samples of the brains of the fly Drosophila melanogaster and other insects, e.g. the jumping ant Harpegnathos saltator. Probes and HCR reagents are purchased from Molecular Instruments. This protocol is loosely based on the "generic sample in solution" protocol published by Molecular Instruments. Our modifications include the description of fixation conditions, counterstaining by Hoechst, and altered washes. Additionally, we use larger concentrations of probes and hairpins following the protocol described by Younger, Herre et al. 2020. We have successfully employed this protocol to stain insect brains with up to 4 different probe sets simultaneously (hairpins conjugated with Alexa Fluor 488, 546, 496, and 647).

Drosophila melanogaster homologs of the raf oncogene

1987 ◽  
Vol 7 (6) ◽  
pp. 2134-2140
Author(s):  
G E Mark ◽  
R J MacIntyre ◽  
M E Digan ◽  
L Ambrosio ◽  
N Perrimon
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Steady State ◽  
Kinase Domain ◽  
Early Embryogenesis ◽  
The Other ◽  
Chromosome 2 ◽  
Related Gene ◽  
Steady State Levels

A murine v-raf probe, representing the kinase domain, was used to identify two unique loci in Drosophila melanogaster DNA. The most closely related to v-raf was mapped by in situ hybridization to position 2F5-6 (Draf-1) on the X chromosome, whereas the other raf-related gene (Draf-2) was found at position 43A2-5 on chromosome 2. The nucleotide and amino acid homologies of Draf-1 to the kinase domain of v-raf are 61 and 65%, respectively. The large amount of a 3.2-kilobase Draf-1 transcript detected in eggs as a maternal message decreases during embryonic development, and significant steady-state levels are observed throughout the remainder of morphogenesis. We speculate that the Draf-1 locus plays an important role in early embryogenesis.

Chromosomal localization of the white gene of Lucilia cuprina (Diptera; Calliphoridae) by in situ hybridization

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 72-75 ◽  
Author(s):  
D. G. Bedo ◽  
A. J. Howells
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Key Words ◽  
Chromosomal Localization ◽  
Polytene Chromosomes ◽  
White Gene ◽  
Lucilia Cuprina ◽  
Standard Methods ◽  
Cytological Data

The white gene of Lucilia cuprina was mapped to trichogen polytene chromosomes using in situ hybridization. A tritium-labelled riboprobe made from the first gene cloned from this species was used with techniques modified from standard methods used for Drosophila melanogaster. Cytological data limiting the location of the white gene to a small portion of 3L and complementing the in situ results are also presented. Key words: Lucilia cuprina, white gene, in situ hybridization.

scholarly journals The random primer labeling technique applied to in situ hybridization on tissue sections.

1988 ◽  
Vol 36 (10) ◽  
pp. 1335-1340 ◽  
Author(s):  
M Thomas-Cavallin ◽  
O Aït-Ahmed
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Specific Activity ◽  
High Specific Activity ◽  
High Sensitivity ◽  
Random Primer ◽  
Tissue Sections ◽  
In Situ Hybridizations ◽  
Labeling Method

We report an application of the random primer labeling technique to in situ hybridizations on tissue sections. The ease of the method and the high specific activity achieved make it valuable when a large number of probes must be analyzed and high sensitivity is needed. We have applied this technique to study the spatial expression of a cluster of maternally acting genes (the yema gene region of Drosophila melanogaster which encodes eleven transcripts, some of them having a very low level of expression) (Aït-Ahmed et al., 1978: Dev Biol 122:153; Aït-Ahmed et al., unpublished results). The results reported here concern one of the transcripts of the yema region, which displays a peculiar anterior localization in the oocyte. We demonstrate that the "oligo-labeling" method allows a far better level of detection of the transcript of interest.

The in situ localization of Adh transcripts in Drosophila species reveals evolved regulatory differences in spatially restricted expression

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 984-991
Author(s):  
Gogineni Ranganayakulu
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Transcriptional Activity ◽  
Fat Body ◽  
Interspecific Variation ◽  
Specific Expression ◽  
Temporal Aspects ◽  
Drosophila Adh

Spatial and temporal aspects of Adh expression were examined during oogenesis and embryogenesis of Drosophila melanogaster, D. simulans, and D. virilis by in situ hybridization. In stage 14 and 15 embryos, differences in zygotic expression of Adh in the primordia of the gastric caecae of D. simulans and in the fat body of D. virilis were observed. These zygotic differences appear to be transient because Adh expression is seen in the gastric caecae of stage 16 embryos of D. simulans and in the fat body of stage 17 embryos of D. virilis. Analysis of D. melanogaster × D. simulans hybrids revealed that the parental difference for transcriptional activity of Adh in the primordia of the gastric caecae is under dominant control. These results provide the basis for exploring evolved regulatory differences in Adh expression during oogenesis and embryogenesis of Drosophila, which are until now unexplored. The potential of in situ hybridization in analyzing evolved regulatory differences in gene expression is briefly discussed.Key words: Drosophila, Adh, tissue-specific expression, interspecific variation, in situ hybridization.

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