scholarly journals Construction and characterization of mismatch-containing circular DNA molecules competent for assessment of nick-directed human mismatch repair in vitro

2002 ◽  
Vol 30 (3) ◽  
pp. 14e-14 ◽  
Author(s):  
E. D. Larson
Keyword(s):  
Mismatch Repair ◽  
Dna Molecules ◽  
Circular Dna

Characterization of circular DNA molecules from cotton plants with leaf curl disease

2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Sohail Akhtar ◽  
Muhammad Nouman Tahir ◽  
Imran Amin ◽  
Rana Binyamin ◽  
Shahid Mansoor
Keyword(s):  
Dna Molecules ◽  
Circular Dna ◽  
Cotton Plants ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Molecular cloning and characterization of avian sarcoma virus circular DNA molecules.

1980 ◽  
Vol 36 (1) ◽  
pp. 50-61 ◽  
Author(s):  
W J DeLorbe ◽  
P A Luciw ◽  
H M Goodman ◽  
H E Varmus ◽  
J M Bishop
Keyword(s):  
Molecular Cloning ◽  
Dna Molecules ◽  
Avian Sarcoma Virus ◽  
Circular Dna ◽  
Sarcoma Virus ◽  
Avian Sarcoma

A calculus for rational tangles: applications to DNA recombination

1990 ◽  
Vol 108 (3) ◽  
pp. 489-515 ◽  
Author(s):  
C. Ernst ◽  
D. W. Sumners
Keyword(s):  
Dna Recombination ◽  
Enzyme Reaction ◽  
Enzyme Mechanism ◽  
Dna Molecules ◽  
Reaction Products ◽  
Circular Dna ◽  
Enzyme Action ◽  
Rational Tangles ◽  
Cellular Dna

There exist naturally occurring enzymes (topoisomerases and recombinases), which, in order to mediate the vital life processes of replication, transcription, and recombination, manipulate cellular DNA in topologically interesting and non-trivial ways [24, 30]. These enzyme actions include promoting the coiling up (supercoiling) of DNA molecules, passing one strand of DNA through another via a transient enzyme-bridged break in one of the strands (a move performed by topoisomerase), and breaking a pair of strands and recombining them to different ends (a move performed by recombinase). An interesting development for topology has been the emergence of a new experimental protocol, the topological approach to enzymology [30], which directly exploits knot theory in an effort to understand enzyme action. In this protocol, one reacts artificial circular DNA substrate with purified enzyme in vitro (in the laboratory); the enzyme acts on the circular DNA, causing changes in both the euclidean geometry (supercoiling) of the molecules and in the topology (knotting and linking) of the molecules. These enzyme-caused changes are experimental observables, using gel electrophoresis to fractionate the reaction products, and rec A enhanced electron microscopy [15] to visualize directly and to determine unambiguously the DNA knots and links which result as products of an enzyme reaction. This experimental technique calls for the building of knot-theoretic models for enzyme action, in which one wishes mathematically to extract information about enzyme mechanism from the observed changes in the DNA molecules.

Characterization of a new class of circular DNA molecules in yeast

1971 ◽  
Vol 42 (3) ◽  
pp. 550-557 ◽  
Author(s):  
Michel Guerineau ◽  
Claude Grandchamp ◽  
Claude Paoletti ◽  
Piotr Slonimski
Keyword(s):  
Dna Molecules ◽  
Circular Dna ◽  
New Class

scholarly journals Validation of an in Vitro Mismatch Repair Assay Used in the Functional Characterization of Mismatch Repair Variants

2020 ◽  
Vol 22 (3) ◽  
pp. 376-385
Author(s):  
Maribel González-Acosta ◽  
Inga Hinrichsen ◽  
Anna Fernández ◽  
Conxi Lázaro ◽  
Marta Pineda ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Functional Characterization
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