scholarly journals MagnaportheDB: a federated solution for integrating physical and genetic map data with BAC end derived sequences for the rice blast fungus Magnaporthe grisea

2002 ◽  
Vol 30 (1) ◽  
pp. 121-124 ◽  
Author(s):  
S. L. Martin
2000 ◽  
Vol 13 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Waly Dioh ◽  
Didier Tharreau ◽  
Jean Loup Notteghem ◽  
Marc Orbach ◽  
Marc-Henri Lebrun

Three genetically independent avirulence genes, AVR1-Irat7, AVR1-MedNoï, and AVR1-Ku86, were identified in a cross involving isolates Guy11 and 2/0/3 of the rice blast fungus, Magnaporthe grisea. Using 76 random progeny, we constructed a partial genetic map with restriction fragment length polymorphism (RFLP) markers revealed by probes such as the repeated sequences MGL/MGR583 and Pot3/MGR586, cosmids from the M. grisea genetic map, and a telomere sequence oligonucleotide. Avirulence genes AVR1-MedNoï and AVR1-Ku86 were closely linked to te-lomere RFLPs such as marker TelG (6 cM from AVR1-MedNoï) and TelF (4.5 cM from AVR1-Ku86). Avirulence gene AVR1-Irat7 was linked to a cosmid RFLP located on chromosome 1 and mapped at 20 cM from the avirulence gene AVR1-CO39. Using bulked segregant analysis, we identified 11 random amplified polymorphic DNA (RAPD) markers closely linked (0 to 10 cM) to the avirulence genes segregating in this cross. Most of these RAPD markers corresponded to junction fragments between known or new transposons and a single-copy sequence. Such junctions or the whole sequences of single-copy RAPD markers were frequently absent in one parental isolate. Single-copy sequences from RAPD markers tightly linked to avirulence genes will be used for positional cloning.


2011 ◽  
Vol 2011 (31) ◽  
pp. 6276-6280 ◽  
Author(s):  
Koji Tanaka ◽  
Ayaka Sasaki ◽  
Hai-Qun Cao ◽  
Teiko Yamada ◽  
Masahiro Igarashi ◽  
...  

PROTEOMICS ◽  
2004 ◽  
Vol 4 (11) ◽  
pp. 3579-3587 ◽  
Author(s):  
Sun Tae Kim ◽  
Seok Yu ◽  
Sang Gon Kim ◽  
Han Ju Kim ◽  
Sun Young Kang ◽  
...  

1999 ◽  
Vol 12 (6) ◽  
pp. 508-514 ◽  
Author(s):  
Birgit Jarosch ◽  
Karl-Heinz Kogel ◽  
Ulrich Schaffrath

Recessive alleles of the barley Mlo locus confer non-race-specific resistance against the powdery mildew fungus Blumeria graminis f. sp. hordei (Bgh). Recently the Mlo gene has been isolated and it was suggested that the Mlo product is a negative regulator of cell death. Thus, loss of function can precondition cells to a higher responsiveness for the onset of multiple defense functions. Here, we document an enhanced susceptibility of barley mlo mutants to the rice blast fungus Magnaporthe grisea. The disease phenotype is independent of the barley cultivar in which the mlo allele has been introgressed and occurs in equal amounts in barley backcross lines of cv. Ingrid carrying the mlo-1, mlo-3, or mlo-5 allele. Ror genes, which are required for the full expression of mlo resistance in barley against Bgh, do not affect the specific mlo-mediated phenotype observed after M. grisea infection. Formation of an effective papilla restricts blast development in epidermal cells of Mlo plants. In contrast, papillae are mostly penetrated in mlo mutants and, as a consequence, the fungus spreads into adjacent mesophyll cells. Both wild-type plants and mlo mutants did not differ in perception of a purified elicitor derived from M. grisea. Thus, we hypothesize that a functional Mlo protein is a prerequisite for penetration resistance of barley to fungal pathogens like M. grisea. The benefit of mlo alleles for durable resistance in barley and a proposed role of mlo-type-mutations in rice are discussed.


1990 ◽  
Vol 17 (5) ◽  
pp. 409-411 ◽  
Author(s):  
Hei Leung ◽  
Ulla Lehtinen ◽  
Reijo Karjalainen ◽  
Daniel Skinner ◽  
Paul Tooley ◽  
...  

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