scholarly journals Directional cDNA library construction assisted by the in vitro recombination reaction

2001 ◽  
Vol 29 (4) ◽  
pp. 22e-22 ◽  
Author(s):  
O. Ohara
Keyword(s):  
Cdna Library ◽  
Recombination Reaction ◽  
Cdna Library Construction ◽  
Library Construction ◽  
In Vitro Recombination

cDNA library construction of two human Demodexspecies

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
DongLing Niu ◽  
RuiLing Wang ◽  
YaE Zhao ◽  
Rui Yang ◽  
Li Hu ◽  
...  
Keyword(s):  
Cdna Library ◽  
Cdna Library Construction ◽  
Library Construction

AbstractThe research of

scholarly journals Purification and Characterization of the R64 Shufflon-Specific Recombinase

2000 ◽  
Vol 182 (10) ◽  
pp. 2787-2792 ◽  
Author(s):  
Atsuko Gyohda ◽  
Teruya Komano
Keyword(s):  
Electrophoretic Analysis ◽  
Recombination Reaction ◽  
Site Specific ◽  
Dna Inversion ◽  
Repeat Sequences ◽  
In Vitro Recombination ◽  
Recombinase Gene ◽  
A Site

ABSTRACT The shufflon, a multiple DNA inversion system in plasmid R64, consists of four invertible DNA segments which are separated and flanked by seven 19-bp repeat sequences. The product of a site-specific recombinase gene, rci, promotes site-specific recombination between any two of the inverted 19-bp repeat sequences of the shufflon. To analyze the molecular mechanism of this recombination reaction, Rci protein was overproduced and purified. The purified Rci protein promoted the in vitro recombination reaction between the inverted 19-bp repeats of supercoiled DNA of a plasmid carrying segment A of the R64 shufflon. The recombination reaction was enhanced by the bacterial host factor HU. Gel electrophoretic analysis indicated that the Rci protein specifically binds to the DNA segments carrying the 19-bp sequences. The binding affinity of the Rci protein to the four shufflon segments as well as four synthetic 19-bp sequences differed greatly: among the four 19-bp repeat sequences, the repeat-a and -d sequences displayed higher affinity to Rci protein. These results suggest that the differences in the affinity of Rci protein for the 19-bp repeat sequences determine the inversion frequencies of the four segments.

cDNA library construction from meristematic tissue of finger millet panicle

2014 ◽  
Vol 48 (5) ◽  
pp. 273-278
Author(s):  
V. Radchuk ◽  
Ya. V. Pirko ◽  
S. V. Isayenkov ◽  
A. I. Yemets ◽  
Ya. B. Blume
Keyword(s):  
Cdna Library ◽  
Finger Millet ◽  
Cdna Library Construction ◽  
Meristematic Tissue ◽  
Library Construction

cDNA Library Construction for the Lambda ZAP®-Based Vectors

2003 ◽  
pp. 355-365
Author(s):  
Marjory A. Snead ◽  
Michelle A. Alting-Mees ◽  
Jay M. Short
Keyword(s):  
Cdna Library ◽  
Cdna Library Construction ◽  
Library Construction
Sign in / Sign up

Export Citation Format

Share Document