scholarly journals DNA-bound transcription factor complexes analysed by mass-spectrometry: binding of novel proteins to the human c-fos SRE and related sequences

2001 ◽  
Vol 29 (2) ◽  
pp. 479-487 ◽  
Author(s):  
V. Drewett
Keyword(s):  
Mass Spectrometry ◽  
Transcription Factor ◽  
Novel Proteins ◽  
Transcription Factor Complexes

Isolation and Characterization of Hematopoietic Transcription Factor Complexes byin VivoBiotinylation Tagging and Mass Spectrometry

2005 ◽  
Vol 1054 (1) ◽  
pp. 55-67 ◽  
Author(s):  
FRANK GROSVELD ◽  
PATRICK RODRIGUEZ ◽  
NATALIA MEIER ◽  
SANJA KRPIC ◽  
FARZIN POURFARZAD ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Transcription Factor ◽  
Isolation And Characterization ◽  
Transcription Factor Complexes

scholarly journals The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Kuo Yang ◽  
Jian-Ping An ◽  
Chong-Yang Li ◽  
Xue-Na Shen ◽  
Ya-Jing Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Transcription Factor ◽  
Liquid Chromatography ◽  
Zinc Finger ◽  
Leaf Senescence ◽  
Transcriptional Activity ◽  
Molecular Mechanisms ◽  
Liquid Chromatography Mass Spectrometry ◽  
Zinc Finger Transcription Factor ◽  
Insight Into

AbstractJasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.

scholarly journals Proteomic Analyses of Vitreous in Proliferative Diabetic Retinopathy: Prior Studies and Future Outlook

2021 ◽  
Vol 10 (11) ◽  
pp. 2309
Author(s):  
Sarah R. Weber ◽  
Yuanjun Zhao ◽  
Christopher Gates ◽  
Jingqun Ma ◽  
Felipe da Veiga Leprevost ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Proteomic Analysis ◽  
Retinal Disease ◽  
Vitreous Fluid ◽  
Future Outlook ◽  
Novel Proteins ◽  
Popular Medium ◽  
Molecular Information

Vitreous fluid is becoming an increasingly popular medium for the study of retinal disease. Numerous studies have demonstrated that proteomic analysis of the vitreous from patients with proliferative diabetic retinopathy yields valuable molecular information regarding known and novel proteins and pathways involved in this disease. However, there is no standardized methodology for vitreous proteomic studies. Here, we share a suggested protocol for such studies and outline the various experimental and analytic methods that are currently available. We also review prior mass spectrometry-based proteomic studies of the vitreous from patients with proliferative diabetic retinopathy, discuss common pitfalls of these studies, and propose next steps for moving the field forward.

scholarly journals Xenopus Smad4β Is the Co-Smad Component of Developmentally Regulated Transcription Factor Complexes Responsible for Induction of Early Mesodermal Genes

1999 ◽  
Vol 214 (2) ◽  
pp. 354-369 ◽  
Author(s):  
Michael Howell ◽  
Fumiko Itoh ◽  
Christophe E. Pierreux ◽  
Sigridur Valgeirsdottir ◽  
Susumu Itoh ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Developmentally Regulated ◽  
Transcription Factor Complexes

scholarly journals Inferring transcription factor complexes from ChIP-seq data

2011 ◽  
Vol 39 (15) ◽  
pp. e98-e98 ◽  
Author(s):  
Tom Whitington ◽  
Martin C. Frith ◽  
James Johnson ◽  
Timothy L. Bailey
Keyword(s):  
Transcription Factor ◽  
Transcription Factor Complexes

scholarly journals Multi-Omics Characterization of Circular RNA-Encoded Novel Proteins Associated With Bladder Outlet Obstruction

2022 ◽  
Vol 9 ◽  
Author(s):  
Baoyi Zhu ◽  
Zhanfang Kang ◽  
Sihua Zhu ◽  
Yuying Zhang ◽  
Xiangmao Lai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Proteomics ◽  
Bladder Outlet Obstruction ◽  
Molecular Mechanisms ◽  
Outlet Obstruction ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Protein Profiles ◽  
Rt Pcr ◽  
Novel Proteins

Bladder outlet obstruction (BOO) is a common urologic disease associated with poorly understood molecular mechanisms. This study aimed to investigate the possible involvements of circRNAs (circular RNAs) and circRNA-encoded proteins in BOO development. The rat BOO model was established by the partial bladder outlet obstruction surgery. Differential expression of circRNA and protein profiles were characterized by deep RNA sequencing and iTRAQ quantitative proteomics respectively. Novel proteins encoded by circRNAs were predicted through ORF (open reading frame) selection using the GETORF software and verified by the mass spectrometry in proteomics, combined with the validation of their expressional alterations by quantitative RT-PCR. Totally 3,051 circRNAs were differentially expressed in bladder tissues of rat BOO model with widespread genomic distributions, including 1,414 up-regulated, and 1,637 down-regulated circRNAs. Our following quantitative proteomics revealed significant changes of 85 proteins in rat BOO model, which were enriched in multiple biological processes and signaling pathways such as the PPAR and Wnt pathways. Among them, 21 differentially expressed proteins were predicted to be encoded by circRNAs and showed consistent circRNA and protein levels in rat BOO model. The expression levels of five protein-encoding circRNAs were further validated by quantitative RT-PCR and mass spectrometry. The circRNA and protein profiles were substantially altered in rat BOO model, with great expressional changes of circRNA-encoded novel proteins.

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