scholarly journals In vivo, high-resolution analysis of yeast and mammalian RNA-protein interactions, RNA structure, RNA splicing and ribozyme cleavage by use of terminal transferase-dependent PCR

2000 ◽  
Vol 28 (7) ◽  
pp. 1656-1664 ◽  
Author(s):  
H.-H. Chen
Keyword(s):  
High Resolution ◽  
Protein Interactions ◽  
Rna Splicing ◽  
Rna Structure ◽  
Terminal Transferase ◽  
High Resolution Analysis ◽  
Resolution Analysis

scholarly journals Microbial Community Development in a Dynamic Gut Model Is Reproducible, Colon Region Specific, and Selective for Bacteroidetes and Clostridium Cluster IX

2010 ◽  
Vol 76 (15) ◽  
pp. 5237-5246 ◽  
Author(s):  
Pieter Van den Abbeele ◽  
Charlotte Grootaert ◽  
Massimo Marzorati ◽  
Sam Possemiers ◽  
Willy Verstraete ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Resolution ◽  
Microbial Community Composition ◽  
Fecal Microbiota ◽  
Microbial Colonization ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Colonization Process

ABSTRACT Dynamic, multicompartment in vitro gastrointestinal simulators are often used to monitor gut microbial dynamics and activity. These reactors need to harbor a microbial community that is stable upon inoculation, colon region specific, and relevant to in vivo conditions. Together with the reproducibility of the colonization process, these criteria are often overlooked when the modulatory properties from different treatments are compared. We therefore investigated the microbial colonization process in two identical simulators of the human intestinal microbial ecosystem (SHIME), simultaneously inoculated with the same human fecal microbiota with a high-resolution phylogenetic microarray: the human intestinal tract chip (HITChip). Following inoculation of the in vitro colon compartments, microbial community composition reached steady state after 2 weeks, whereas 3 weeks were required to reach functional stability. This dynamic colonization process was reproducible in both SHIME units and resulted in highly diverse microbial communities which were colon region specific, with the proximal regions harboring saccharolytic microbes (e.g., Bacteroides spp. and Eubacterium spp.) and the distal regions harboring mucin-degrading microbes (e.g., Akkermansia spp.). Importantly, the shift from an in vivo to an in vitro environment resulted in an increased Bacteroidetes/Firmicutes ratio, whereas Clostridium cluster IX (propionate producers) was enriched compared to clusters IV and XIVa (butyrate producers). This was supported by proportionally higher in vitro propionate concentrations. In conclusion, high-resolution analysis of in vitro-cultured gut microbiota offers new insight on the microbial colonization process and indicates the importance of digestive parameters that may be crucial in the development of new in vitro models.

Very High Resolution Analysis of the Dynamics of a Coronal Plasmoid

1994 ◽  
Vol 144 ◽  
pp. 593-596
Author(s):  
O. Bouchard ◽  
S. Koutchmy ◽  
L. November ◽  
J.-C. Vial ◽  
J. B. Zirker
Keyword(s):  
High Resolution ◽  
Solar Eclipse ◽  
Total Solar Eclipse ◽  
Field Of View ◽  
Small Field ◽  
High Resolution Analysis ◽  
Ccd Observations ◽  
Resolution Analysis ◽  
Very High ◽  
Small Field Of View

AbstractWe present the results of the analysis of a movie taken over a small field of view in the intermediate corona at a spatial resolution of 0.5“, a temporal resolution of 1 s and a spectral passband of 7 nm. These CCD observations were made at the prime focus of the 3.6 m aperture CFHT telescope during the 1991 total solar eclipse.

scholarly journals How to Effectively Control Energy Consumption Growth in China’s 29 Provinces: A Paradigm of Multi-Regional Analysis Based on EAALMDI Method

2021 ◽  
Vol 13 (3) ◽  
pp. 1093
Author(s):  
Yunlong Zhao ◽  
Geng Kong ◽  
Chin Hao Chong ◽  
Linwei Ma ◽  
Zheng Li ◽  
...  
Keyword(s):  
Energy Consumption ◽  
High Resolution ◽  
Energy Policy ◽  
Policy Making ◽  
Regional Analysis ◽  
Policy Implications ◽  
Consumption Growth ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Consumption Control

Controlling energy consumption to reduce greenhouse gas emissions has become a global consensus in response to the challenge of climate change. Most studies have focused on energy consumption control in a single region; however, high-resolution analysis of energy consumption and personalized energy policy-making, for multiple regions with differentiated development, have become a complicated challenge. Using the logarithmic mean Divisia index I (LMDI) decomposition method based on energy allocation analysis (EAA), this paper aims to establish a standard paradigm for a high-resolution analysis of multi-regional energy consumption and provide suggestions for energy policy-making, taking 29 provinces of China as the sample. The process involved three steps: (1) determination of regional priorities of energy consumption control by EAA, (2) revealing regional disparity among the driving forces of energy consumption growth by LMDI, and (3) deriving policy implications by comparing the obtained results with existing policies. The results indicated that 29 provinces can be divided into four groups, with different priorities of energy consumption control according to the patterns of coal flows. Most provinces have increasing levels of energy consumption, driven by increasing per capita GDP and improving living standards, while its growth is restrained by decreasing end-use energy intensity, improving energy supply efficiency, and optimization of industrial structures. However, some provinces are not following these trends to the same degree. This indicates that policy-makers must pay more attention to the different driving mechanisms of energy consumption growth among provinces.

scholarly journals A Back Migration from Asia to Sub-Saharan Africa Is Supported by High-Resolution Analysis of Human Y-Chromosome Haplotypes

2002 ◽  
Vol 70 (5) ◽  
pp. 1197-1214 ◽  
Author(s):  
Fulvio Cruciani ◽  
Piero Santolamazza ◽  
Peidong Shen ◽  
Vincent Macaulay ◽  
Pedro Moral ◽  
...  
Keyword(s):  
High Resolution ◽  
Y Chromosome ◽  
Sub Saharan Africa ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Sub Saharan ◽  
Human Y Chromosome

scholarly journals Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aleksandar Antanasijevic ◽  
Leigh M. Sewall ◽  
Christopher A. Cottrell ◽  
Diane G. Carnathan ◽  
Luis E. Jimenez ◽  
...  
Keyword(s):  
High Resolution ◽  
Polyclonal Antibody ◽  
Vaccine Development ◽  
Mutational Analysis ◽  
Rhesus Macaques ◽  
Antibody Responses ◽  
Glycosylation Sites ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Resolution Mapping

AbstractEngineered ectodomain trimer immunogens based on BG505 envelope glycoprotein are widely utilized as components of HIV vaccine development platforms. In this study, we used rhesus macaques to evaluate the immunogenicity of several stabilized BG505 SOSIP constructs both as free trimers and presented on a nanoparticle. We applied a cryoEM-based method for high-resolution mapping of polyclonal antibody responses elicited in immunized animals (cryoEMPEM). Mutational analysis coupled with neutralization assays were used to probe the neutralization potential at each epitope. We demonstrate that cryoEMPEM data can be used for rapid, high-resolution analysis of polyclonal antibody responses without the need for monoclonal antibody isolation. This approach allowed to resolve structurally distinct classes of antibodies that bind overlapping sites. In addition to comprehensive mapping of commonly targeted neutralizing and non-neutralizing epitopes in BG505 SOSIP immunogens, our analysis revealed that epitopes comprising engineered stabilizing mutations and of partially occupied glycosylation sites can be immunogenic.

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